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A multiple linear regression analyses were performed to screen for the significant factors simultaneously influencing production of delta-endotoxin, proteolytic activities and spore formation by a Bacillus thuringiensis kurstaki strain. Investigated factors included: pH of the medium, available oxygen and inoculum size. It was observed that oxygen availability was the most influencing setting on both deltaendotoxins production and spores counts, followed by initial pH of the medium and inoculum size. On other hand, pH of medium was found to be the most significant parameter for proteolytic activity, followed by inoculum size and dissolved oxygen. Our results suggested that the first order with two-factor interaction model seemed to be more satisfactory than simple first order model for optimization of delta-endotoxin overproduction. The coefficients of determination (R²) indicated a better adequacy of the second order models to justify the obtained data. Based on results, relationships between delta-endotoxins production, proteolytic activities and spores counts were established. Our results can help to balance delta-endotoxins production and its stability.
This paper emphasizes the need of fewer cars use. With the help of current state and the optimal level of product engineering needs of the selected car comparison, setting its overproduction, environmental impacts are quantified, also environmental and economic benefits of fewer cars using are outlined. This paper is also looking for innovative analysis of vehicle downtime. With the help of simple calculations based on continuous recording of the selected parking area analysis value of stoppageness of product and selected phase of its life cycle was implemented. Description of its redundancy is based on stocks theory, for which assistance need for cars with aid of logistics chains for these products has been written.
In this work we present cloning and overexpression of lactococcal CcpA protein in Escherichia coli Xllblue strain as a fusion with 6xHis tag. A high yield of the CcpA protein was obtained when the cells were cultured in liquid medium LB with 100 ug/ml ampicillin at 37°C and subsequently for 4 h after induction by IPTG. The proce­dure let us obtain 5 mg of homogenous CcpA protein. Glutaraldehyde crosslinking analysis indicated the formation of dimer or tetramer forms of the CcpA protein.
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