Wyniki wyszukiwania

1

Overexpression of 4.1R disturbs microtubule organization

100%

Perez-Ferreiro C. M., Luque C. M., Perez-Gonzales A., Sendino E., Correas I.

Cellular and Molecular Biology Letters

|

2001

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tom 06

|

nr 2

2

Cyclooxygenase-2 as a biomarker with diagnostic, therapeutic, prognostic, and predictive relevance in small animal oncology

75%

Szweda M., Rychlik A., Babinska I., Pomianowski A.

Journal of Veterinary Research

|

2020

|

tom 64

|

nr 1

3

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Determination of epoxides in Escherichia coli expressing recombinant zeaxanthin epoxidase

75%

Kuczynska P., Leskiewicz S., Strzalka W., Olchawa-Pajor M., Bojko M., Latowski D., Strzalka K.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2013

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tom 94

|

nr 3

4

Overexpression of human CUTA isoform2 enhances the cytotoxicity of copper to HeLa cells

63%

Yang J., Li Q., Yang H., Yan L., Yang L., Yu L.

Acta Biochimica Polonica

|

2008

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tom 55

|

nr 2

411-415

Copper, an essential transient element, can be toxic to cells when present in excess. Altered copper homeostasis is involved in pathological events of many diseases. Human CUTA isoform2 is a member of cation tolerance protein (CutA1) family. In this study, we examined the effect of CUTA isoform2 overexpression on copper toxicity. It was shown that overexpressed CUTA isoform2 sensitized HeLa cells to copper toxicity by promoting copper-induced apoptosis. The inhibition effect of excessive copper on cell proliferation was also enhanced by overexpressed CUTA isoform2. So CUTA isoform2 was implicated to be involved in the cytotoxicity of copper.

5

Oxidative stress responses are regulated by heat shock factor HSFA4A in Arabidopsis

63%

Perez Salamo I., Papdi C., Rigo G., Zsigmond L., Vilela B., Pages M., Nagy I., Domoki M., Darula Z., Koncz C., Szabados L.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2013

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tom 94

|

nr 2

6

Kynuramines induce overexpression of heat shock proteins in pancreatic cancer cells via 5-hydroxytryptamine and melatonin MT1/MT2 receptors

63%

Leja-Szpak A., Pierzchalski P., Goralska M., Nawrot-Porabka K., Bonior J., Link-Lenczowski P., Jastrzebska M., Jaworek J.

Journal of Physiology and Pharmacology

|

2015

|

tom 66

|

nr 5

7

Overexpression of the Her-2 oncogene in human breast cancer

63%

Corell B., Horst-Sikorska W., Fischer G., Zoll B.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1991

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tom 39

|

nr 2

8

p53-dependent suppression of the human calcyclin gene [S100A6]: the role of Sp1 and of NFkappaB

63%

Kroliczak W., Pietrzak M., Puzianowska-Kuznicka M.

Acta Biochimica Polonica

|

2008

|

tom 55

|

nr 3

559-570

Calcyclin (S100A6) is believed to participate in cell cycle control. It was, however, unclear if its expression depends on p53, a key regulator of apoptosis and cell cycle. We therefore performed transcription regulation assays in HeLa cells and found that wild type p53 suppressed the S100A6 promoter up to 12-fold in a dose-dependent manner. In contrast, the well-characterized V143A, R175H, R249S, and L344A p53 mutants cloned from human cancers suppressed this promoter with a 6 to 9-fold lower efficiency. All the sites mediating the p53-dependent suppression were contained in the –167 to +134 fragment of the S100A6 promoter. Separate overexpression of either Sp1 or of NFκB only partially counteracted the p53 inhibitory effect on the S100A6 promoter, while simultaneous overexpression of both these transactivators resulted in a complete abolishment of the p53 inhibitory effect on this promoter. Sp1 and NFκB binding to the probes resembling their putative binding sites present in the S100A6 promoter was decreased in the presence of wild type p53. We propose that the suppression of S100A6 is yet another mechanism by which p53 inhibits proliferation. Insufficient suppression of this gene by p53 mutants could well be responsible for calcyclin overexpression and cell cycle deregulation observed in cancer tissues.

9

Effect of feeding potato tubers modified by 14-3-3 protein overexpression on metabolism and health status of rats

63%

Kosieradzka I., Sawosz E., Pastuszewska B., Zuk M., Szopa J., Bielecki W.

Journal of Animal and Feed Sciences

|

2004

|

tom 13

|

nr 2

329-339

10

Fibroblast cell shape and adhesion in vitro is altered by overexpression of the 7A and 7B isoforms of protocadherin 7, but not the 7C isoform

63%

Yoshida K.

Cellular and Molecular Biology Letters

|

2003

|

tom 08

|

nr 3

Protocadherins (Pcdhs) are a family of cadherins considered to play an important role in the cell-cell adhesion of specific neurons in the central nervous system. Of the reported Pcdhs, relatively little is known about the functional role of protocadherin 7 (Pcdh7), and there is no evidence of Pcdh7 mediated cell-cell adhesion. To date, three splicing variants are known; they may have different effects on cell phenotype. We report here that mouse fibroblast L cells stably overexpressing the Pcdh7 isoforms 7a and 7b, but not 7c, showed a morphological change and Ca2+ dependent cell adhesion.

11

Overproduction and purification of the CepA protein from Lactococcus lactis

63%

Kowalczyk M., Bardowski J.

Acta Biochimica Polonica

|

2003

|

tom 50

|

nr 2

In this work we present cloning and overexpression of lactococcal CcpA protein in Escherichia coli Xllblue strain as a fusion with 6xHis tag. A high yield of the CcpA protein was obtained when the cells were cultured in liquid medium LB with 100 ug/ml ampicillin at 37°C and subsequently for 4 h after induction by IPTG. The procedure let us obtain 5 mg of homogenous CcpA protein. Glutaraldehyde crosslinking analysis indicated the formation of dimer or tetramer forms of the CcpA protein.

12

Rebamipide attenuates nonsteroidal anti-inflammatory drugs (NSAID) induced lipid peroxidation by the manganese superoxide dismutase (MnSOD) overexpression in gastrointestinal epithelial cell

51%

Nagano Y., Matsui H., Shimokawa O., Hirayama A., Tamura M., Nakamura Y., Kaneko T., Rai K., Indo H. P., Majima H. J., Hyodo I.

Journal of Physiology and Pharmacology

|

2012

|

tom 63

|

nr 2

13

Overexpression of glycosylated proteins in cervical cancer recognized by the Machaerocereus eruca agglutinin

51%

Solorzano C., Mayoral M. A., Carlos M. A., Berumen J., Guevara J., Chavez F. R., Mendoza-Hernandez G., Agundis C., Zenteno E.

Folia Histochemica et Cytobiologica

|

2012

|

tom 50

|

nr 3

14

Wplyw nadekspresji ferrytyny w skielkowanych ziarniakach pszenicy zwyczajnej na tempo rozwoju wolka zbozowego [Sitophilus granarius L.]

51%

Zielinska-Dawidziak M., Gawlak M., Warchalewski J. R.

Progress in Plant Protection

|

2007

|

tom 47

|

nr 4

262-266

15

Survivin is a key factor in the differential susceptibility of gastric endothelial and epithelial cells to alcohol-induced injury

51%

Jones M. K., Padilla O. R., Zhu E.

Journal of Physiology and Pharmacology

|

2010

|

tom 61

|

nr 3

253-264

We previously demonstrated that the anti-apoptosis protein, survivin, plays a protective role against alcohol-induced gastric injury. Since the endothelium is a primary target of alcohol-induced gastric damage, we investigated whether survivin expression is a key factor in the greater susceptibility of gastric endothelial vs. epithelial cells to alcohol-induced injury. Here, we demonstrate that rat gastric epithelial cells (RGM1 cells, an epithelial cell line derived from normal rat gastric mucosa) expressed 7.5-fold greater survivin protein levels vs. rat gastric endothelial cells. Survivin expression correlated with resistance of gastric epithelial vs. endothelial cells to both alcohol-induced cell damage and alcohol-induced apoptosis. Suppression of survivin protein expression levels using siRNA rendered the gastric epithelial cells as susceptible to both alcohol-induced cell damage and apoptosis as the gastric endothelial cells. Conversely, forced overexpression of survivin by transient transfection rendered gastric endothelial cells as resistant to both alcohol-induced cell damage and apoptosis as mock-transfected gastric epithelial cells. Moreover, overexpression of a threonine-34 to glutamate phosphorylation mimic mutant survivin construct rendered gastric endothelial cells significantly more resistant to alcohol-induced damage and apoptosis vs. mock-transfected gastric epithelial cells. These findings indicate that disparate survivin expression levels can explain the discrepancy between gastric epithelial and endothelial cell susceptibility to alcohol-induced injury; and, that a negative charge at amino acid residue 34 on survivin, such as that which naturally occurs by phosphorylation of threonine-34, enhances its property in conferring gastric mucosal protection.

16

Overexpression of suadea salsa S-adenosylmethionine synthetase gene promotes salt tolerance in transgenic tobacco

51%

Qi Y.-C., Wang F.-F., Zhang H., Liu W.-Q.

Acta Physiologiae Plantarum

|

2010

|

tom 32

|

nr 2

The suadea salsa full-length S-adenosylmethionine synthetase (SsSAMS2) was introduced into tobacco (Nicotiana tabacum L.) by Agrobacterium tumefaciens-mediated transformation. The gene transformation and expression in tobacco were confirmed by PCR, RT-PCR and Northern blotting analysis. Several transgenic lines (ST lines) overexpressing SsSAMS2 gene under the control of cauliflower mosaic virus 35S promoter showed more seeds number and weight, and accumulated higher free total polyamines (PAs) than wild-type plants (WT lines) and transformants with blank vector (BT lines). Salt stress-induced damage was attenuated in these transgenic plants, in the symptom of maintaining higher photosynthetic rate and biomass. These results that the transgenic plants overexpressing suadea salsa SAMS2 are more tolerant to salt stress than wild-type plants suggest that PAs may play an important role in contributing salt tolerance to plants.

17

Overexpression of human osteopontin increases cell proliferation and migration in human embryo kidney-293 cells

51%

Liu Y. J., Zhang D.-Q., Sui X.-M., Tian W.

Cellular and Molecular Biology Letters

|

2009

|

tom 14

|

nr 4

670-678

Malignant tumors are characterized by dysregulated cell growth and the metastasis of secondary tumors. Numerous studies have documented that osteopontin (OPN) plays a key role in regulating tumor progression and metastasis. Here, we show that the overexpression of OPN in human embryo kidney-293 cells significantly increases both the level of cell proliferation, by provoking the G1/S transition, and the level of cell migration in vitro. These findings suggest that augmented OPN contributes to cell growth and motility. Inhibiting OPN or the pathway it stimulates may therefore represent a novel approach for the treatment of primary tumors and associated metastases.

18

Fragile histidine triad (FHIT) gene is overexpressed in colorectal cancer

51%

Wierzbicki P. M., Adrych K., Kartanowicz D., Dobrowolski S., Stanislawowski M., Chybicki J., Godlewski J., Korybalski B., Smoczynski M., Kmiec Z.

Journal of Physiology and Pharmacology. Supplement

|

2009

|

tom 60

|

nr 4

19

Overexpression of gtfbeta gene fragments from Streptococcus mutans encoding Glu and Cat domains in Escherichia coli

51%

Lipinski D., Zandecka-Dziubak J., Juzwa W., Zeyland J., Mrozikiewicz P. M., Slomski R.

Herba Polonica

|

2004

|

tom 50

|

nr 2

Streptococcus mutans is involved in the initiation and progression of dental caries. Fragments of a gtfB gene from Streptococcus mutans encoding Glu and Cat peptides of approximately 35 and 45 kDa, respectively, were cloned from this organism and sequenced. Recombinant Glu and Cat peptides were overexpressed in Escherichia coli with C-terminal sequence containing additional six histidine residues and enterokinase recognition site. The recombinant peptides were purified from E. coli by metal affinity chromatography. These proteins will be used to prepare efficient vaccine against dental caries.

20

Inducible overexpression of cytosolic 5'nucleotidases in mammalian cells

51%

Gazziola C., Moras M., Ferraro P., Rampazzo C., Gallinaro L., Reichard P., Bianchi V.

Cellular and Molecular Biology Letters

|

1999

|

tom 04

|

nr 3

Ograniczanie wyników

Overexpression of 4.1R disturbs microtubule organization

Cyclooxygenase-2 as a biomarker with diagnostic, therapeutic, prognostic, and predictive relevance in small animal oncology

Determination of epoxides in Escherichia coli expressing recombinant zeaxanthin epoxidase

Overexpression of human CUTA isoform2 enhances the cytotoxicity of copper to HeLa cells

Oxidative stress responses are regulated by heat shock factor HSFA4A in Arabidopsis

Kynuramines induce overexpression of heat shock proteins in pancreatic cancer cells via 5-hydroxytryptamine and melatonin MT1/MT2 receptors

Overexpression of the Her-2 oncogene in human breast cancer

p53-dependent suppression of the human calcyclin gene [S100A6]: the role of Sp1 and of NFkappaB

Effect of feeding potato tubers modified by 14-3-3 protein overexpression on metabolism and health status of rats

Fibroblast cell shape and adhesion in vitro is altered by overexpression of the 7A and 7B isoforms of protocadherin 7, but not the 7C isoform

Overproduction and purification of the CepA protein from Lactococcus lactis

Rebamipide attenuates nonsteroidal anti-inflammatory drugs (NSAID) induced lipid peroxidation by the manganese superoxide dismutase (MnSOD) overexpression in gastrointestinal epithelial cell

Overexpression of glycosylated proteins in cervical cancer recognized by the Machaerocereus eruca agglutinin

Wplyw nadekspresji ferrytyny w skielkowanych ziarniakach pszenicy zwyczajnej na tempo rozwoju wolka zbozowego [Sitophilus granarius L.]

Survivin is a key factor in the differential susceptibility of gastric endothelial and epithelial cells to alcohol-induced injury

Overexpression of suadea salsa S-adenosylmethionine synthetase gene promotes salt tolerance in transgenic tobacco

Overexpression of human osteopontin increases cell proliferation and migration in human embryo kidney-293 cells

Fragile histidine triad (FHIT) gene is overexpressed in colorectal cancer

Overexpression of gtfbeta gene fragments from Streptococcus mutans encoding Glu and Cat domains in Escherichia coli

Inducible overexpression of cytosolic 5'nucleotidases in mammalian cells