Wyniki wyszukiwania

1

The silver-staining proteins [Ag-NOR proteins] in nucleolar organizer regions of ovarian and testicular cells

100%

Bilinski S. M., Bilinska B.

Acta Biologica Cracoviensia. Series Botanica et Zoologia. Supplement

|

1997

|

tom 39

|

nr 1

2

ERK 1/2 and PI-3 kinase pathways as a potential mechanism of ghrelin action on cell proliferation and apoptosis in the porcine ovarian follicular cells

84%

Rak-Mardyla A., Gregoraszczuk E. L.

Journal of Physiology and Pharmacology

|

2010

|

tom 61

|

nr 4

451-458

Recently, we reported the stimulatory effect of ghrelin on ovarian cell proliferation in parallel with the inhibitory action of ghrelin on cell apoptosis. The aim of the presented data propose local activation of extracellular signal-regulated protein kinase 1 and 2 (ERK 1/2) and phosphoinositide-3 (PI-3) kinase pathways as a mechanism of ghrelin effect in the porcine ovary. To test this hypothesis, action of ghrelin on levels of ERK 1/2 with PI-3 kinase activity and protein expression using ELISA and western blot analysis, respectively, was examined. Additionally, to determine which pathways (ERK 1/2 or PI-3 kinase) are the potential signals of ghrelin-mediated cell proliferation and apoptosis in ovarian cells, we used PD098059 (50 µM) and wortmannin (200 µM), well-known inhibitors of these kinases. Treatment of ovarian coculture cells with ghrelin (100, 250, 500 and 1000 pg/ml) showed stimulation of phospho-ERK 1/2 levels and PI-3 kinase activity, with the maximum effect observed after 15 min of cell incubation. Additionally, western blot analysis indicated that ghrelin increased expression of both kinases. Moreover, ghrelin used in combination with PD098059 or wortmannin significantly decreased cell proliferation, which was measured by the Alamar Blue assay and increased apoptosis, which was measured by caspase - 3 activity and DNA fragmentation. In conclusion, these results suggest that the ERK 1/2 and PI-3 kinase pathways may be potential signals of ghrelin mediate the cell proliferation and apoptosis of ovary cells.

3

Histopathological examination of ovaries in bitches after experimental zearalenone mycotoxicosis

67%

Gajecka M., Obremski K., Jakimiuk E., Skorska-Wyszynska E., Zielonka L., Gajecki M.

Polish Journal of Veterinary Sciences

|

2008

|

tom 11

|

nr 4

363-366

The present study deals with the influence of experimental ZEA mycotoxicosis on histopathological lesions in ovaries of bitches, which were administered zearalenoneperos during anestrus phase for one hundred days. The experiment was performed on 9 sexually mature, clinically healthy bitches. The animals assigned into two experimental groups received zearalenone per os at two doses, 25 μg/kg b.w. and 50 μg/kg b.w., respectively: the bitches from control group received placebo per os. On the last day of zearalenone intoxication, the bitches were ovariohystorectomized. Histopathology and immunohistochemistry were performed. The study revealed that zearalenone and its metabolites caused profound regressive lesions: granular cells degeneration and atrophy. Numerous edemas and blood extravasations were also found. The intensity of these changes was significantly dose dependent. Furthermore, in ovarian cells and tissues of both experimental groups, no reaction for PCNA antigen was observed. In conclusion, zearalenone and its metabolites exerts unfavorable effects on the morphology of ovaries in bitches.

4

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Effect of genistein, tyrphostin and herbimycin on prolactin-stimulated progesterone production by porcine theca and luteal cells

67%

Gregoraszczuk E., Slomczynska M., Stoklosowa S.

Journal of Physiology and Pharmacology

|

1999

|

tom 50

|

nr 3

5

Beauvericin cytotoxicity to the invertebrate cell line SF-9

67%

Calo L., Fornelli F., Nenna S., Tursi A., Caiaffa M. F., Macchia L.

Journal of Applied Genetics

|

2003

|

tom 44

|

nr 4

6

Synthesis of aminophospholipids in Saccharomyces cerevisiae and Chinese hamster ovary cells. From mutagenesis to genes and cellular function

59%

Lenart J., Pikula S.

Cellular and Molecular Biology Letters

|

1998

|

tom 03

|

nr 2

The purpose of this article is to provide a concise overview of the characterization of auxotrophic mutated cells to the precursors of lipid synthesis, and of the identification of specific genes encoding enzymatic proteins involved in this process. The focus is on enzymes catalyzing the synthesis of phosphatidylserine and phosphatidylethanolamine in Saccharomyces cerevisiae and Chinese hamster ovary cells, two cell types frequently used by investigators studying the mechanisms of genetic control of metabolic processes.

7

Synthesis of aminophospholipids in Saccharomyces cerevisiae and Chinese hamster ovary cells. From mutagenesis to genes and cellular function

51%

Lenart J., Pikula S.

Cellular and Molecular Biology Letters

|

1998

|

tom 03

|

nr 3

The purpose of this article is to provide a concise overview of the characterization of auxotrophic mutated cells to the precursors of lipid synthesis, and of the identification of specific genes encoding enzymatic proteins involved in this process. The focus is on enzymes catalyzing the synthesis of phosphatidylserine and phosphatidylethanolamine in Saccharomyces cerevisiae and Chinese hamster ovary cells, two cell types frequently used by investigators studying the mechanisms of genetic control of metabolic processes.

Ograniczanie wyników

The silver-staining proteins [Ag-NOR proteins] in nucleolar organizer regions of ovarian and testicular cells

ERK 1/2 and PI-3 kinase pathways as a potential mechanism of ghrelin action on cell proliferation and apoptosis in the porcine ovarian follicular cells

Histopathological examination of ovaries in bitches after experimental zearalenone mycotoxicosis

Effect of genistein, tyrphostin and herbimycin on prolactin-stimulated progesterone production by porcine theca and luteal cells

Beauvericin cytotoxicity to the invertebrate cell line SF-9

Synthesis of aminophospholipids in Saccharomyces cerevisiae and Chinese hamster ovary cells. From mutagenesis to genes and cellular function

Synthesis of aminophospholipids in Saccharomyces cerevisiae and Chinese hamster ovary cells. From mutagenesis to genes and cellular function