The aim of the study has been to define the stocks and the basic properties of soil organic matter in agricultural-used soil sequence located in the former shoreline of disappearing lakes. The study area is located in the catchment of the Sumowskie Lakes, the Brodnica Lake District, North-Eastern Poland. The investigations involved preparation of five soil pits located in the south-western part of the former Sumowskie Lake bottom and on the southern slope of the adjacent kame hill. The greatest soil transformation is reflected in the quality and quantity of the soil organic matter. Indexes of organic matter quality and TOC stocks are significantly changing along the studied transect. The strongly humidified organic matter is found in mursh horizons. Gyttja layers above the groundwater level have a medium humification index. Horizons saturated with water are very low humidified. TOC stocks drop along the analysed soil sequence from the biogenic plain to the top of the kame hill.