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Studies of plant breeding systems are particularly important when they involve facultative processes, which can be overlooked in natural conditions. This especially applies to species that are considered allogamous, due to their pollination syndrome and floral architecture. We examined the potential level and factors enabling or limiting spontaneous autogamy in three populations of the lepidopteran orchid Gymnadenia conopsea. Using a bagging experiment, we noted the stages of the anther thecae and the positions of the pollinaria at the five phenological stages of the flower, as well as the quantity of autogamously set fruits and the number of properly formed seeds. In the studied populations, autogamy represents an accidental character, with a maximum of 3.3% of fruits set spontaneously per analysed sample in a given population, and with seed numbers ranging from 29 to 354. This process is an environmentally dependent co-product of the mechanisms that enable a position appropriate for touching the stigma (bending of the caudicle) and increase male fitness (disintegration of the massulae), preceded by the gradual opening of the anther chambers. Autopollination of G. conopsea may occur in the flowers at various flowering stages (excluding the beginning of anthesis) at each position on the inflorescence.
Most orchids do not produce floral food-rewards. Instead, they attract pollinators by mimicry or deceit. When present, the most common floral food-reward is nectar. To date, nectary structure has been described for only two species of Maxillaria sensu lato, namely Maxillariella anceps and Ornithidium coccineum (formerly Maxillaria anceps and M. coccinea, respectively). Here, we describe that of a third species, Ornithidium sophronitis (formerly Maxillaria sophronitis). This species possesses floral characters concomitant with ornithophily. A ‘faucet and sink’ arrangement is present, with nectar secreted by a protuberance on the ventral surface of the column, collecting between column and tepal bases. The nectary of O. sophronitis shares many features with that of O. coccineum. It has a single-layered epidermis and 3- 5 layers of small, subepidermal, collenchymatous, secretory cells. Beneath these occur 2-3 layers of larger, subsecretory, parenchymatous cells supplied by phloem. Nectary cell vacuoles contain osmiophilic material and proteinaceous intravacuolar bodies. Moreover, distension of the nectary cuticle occurs as nectar accumulates between it and the secretory epidermis. Subsecretory cells, however, have thinner walls and contain flocculent, intravacuolar precipitates that may be related to the presence of flavonoids. Since the floral and nectary structure of O. sophronitis is very similar to that of closely related Ornithidium coccineum, it may have evolved in like manner in response to similar pollinator pressures.
The physiological response of Phalaenopsis × hybridum ‘Innocence’ to biotic stress caused by Pseudococcus longispinus feeding was investigated. The condition of the cytoplasmic membranes expressed by a value of electrolyte outflow (EL) and TBARS and the activity of antioxidative system enzymes: catalase and peroxidase, and the amount of non-enzymatic antioxidant – proline, were determined. The changes in all the analyzed physiological parameters depended on the duration of the pest feeding. The outflow of electrolytes, TBARS content and catalase activity was the highest in the first period of the experiment (after 24-hour of mealybug feeding). Significant increase of peroxidase activity and proline content was noted after 7 days of insects feeding. The values of all analyzed parameters (except EL) demonstrated a decreasing tendency after 14 days of P. longispinus feeding. The observed reaction of P. hybridum ‘Innocence’ testifies to mechanisms triggered with the aim of neutralizing the effects of biotic stress and enabling the normal functioning of the cells in the orchid plants colonized by longtailed mealybug.
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