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Investigation of the influence of winter wheat’s location on plant’s germination energy

100%

Zubko V., Kuzina T.

Teka Komisji Motoryzacji i Energetyki Rolnictwa

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2015

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tom 15

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nr 3

2

Elaboration of optimal conditions for micropropagation and cryopreservation of garlic [Allium sativum L.]

100%

Makowska Z., Kotlinska T.

Vegetable Crops Research Bulletin

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2001

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tom 54

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nr 1

3

Partial characterization of a lipase from gypsy moth [Lymantria dispar L.] larval midgut

84%

Mrdakovic M., Lazarevic J., Peric-Mataruga V., Ilijin L., Vlahovic M.

Folia Biologica

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2008

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tom 56

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nr 1-2

103-110

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Optimization of the technique for the short-term culture of equine hepatocytes

84%

Bakala A., Karlik W., Wiechetek M., Grono D., Glowala A.

Medycyna Weterynaryjna

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2007

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tom 63

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nr 11 Supl.

1440-1442

The aim of this study was to establish the optimal conditions for the culture of equine hepatocytes in a monolayer configuration. The obtained results show that the rate of MTT metabolism correlated with the number of cultured cells and a linear increase of MTT reduction rate was observed in cases when the cell density varied between 1.25 × 10⁴ to 5 × 10⁴ viable cell/well of 96-well plate. Hepatocytes reached the optimal cell attachment rate and MTT reduction at a cell density of 5 × 10⁴ cells/well. The number of attached cells to a plastic culture dish was also related to incubation time. The greatest ability of hepatocytes to attach to the culture dish was observed after 10 h of incubation and it was found to be 84.1 ± 2.5% of seeded hepatocytes. It was also found that fetal bovine serum was more efficient than horse serum for the attachment of equine isolated hepatocytes in a monolayer culture. The highest rate of cell attachment (assessed microscopically and with MTT reduction test) was observed when cells were plated with the culture medium supplemented with FBS or HS at a concentration of 5%. However, medium supplementation with higher than 5% serum concentration (10% of FBS or HS) significantly decreased MTT reduction rate. The rate of MTT metabolism and cell attachment in hepatocytes cultured in WE supplemented with FBS or HS was also dependent on the plating time and were the highest after 10 h of seeding.

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Oxygen consumption and ammonia excretion by Eurasian perch (Perca fluviatilis L.) reared under optimal thermal conditions

84%

Zakes Z., Demska-Zakes K.

Archives of Polish Fisheries

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2005

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tom 13

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nr 2

The aim of the study was to determine the oxygen consumption (OC, mg O₂ kg⁻¹h⁻¹) and ammonia production (AE, mg TAN kg⁻¹h⁻¹) of perch reared in a recirculating system at the optimal temperature for this species of 23°C. The studies were conducted when the fish were undergoing intense fattening on artificial feed. Measurements of metabolic rate were taken 24 hours per day in six size groups of fish (mean body weights (BW) in each group - 18.4, 30.7, 46.4, 56.5, 67.8, 82.3 g). Within the studied perch size range, OC decreased from 336.2 to 185.0 mg O₂ kg⁻¹h⁻¹, and AE from 22.0 to 5.6 mg TAN kg⁻¹h⁻¹. An increase in body weight by 1 g led to an average decrease in oxygen consumption by a mean of 2.53 mg O₂ kg⁻¹h⁻¹ and ammonia excretion by 0.28 mg TAN kg⁻¹ h⁻¹. During the analyzed period, the body weight of perch, the arithmetic dependence between OC-BW and AE-BW, was linear and the determination coefficients R² of the linear regression equations describing these relationships were highly statistically significant at a value exceeding 0.9.

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Stimulatory effect of Asahi SL on selected plant species

67%

Przybysz A., Wrochna M., Slowinski A., Gawronska H.

Acta Scientiarum Polonorum. Hortorum Cultus

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2010

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tom 09

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nr 2

Asahi SL stimulates plant’s vital processes like growth and development, affects physiology and biochemistry, what often leads to increased biomass accumulation and yield. However, common is opinion that application of this preparation could be beneficial only, when treated plants are grown under unfavorable conditions. Therefore the aim of this work was the assessment of the stimulatory effect of Asahi SL on Arabidopsis thaliana L. and ornamental amaranth plants grown under optimal conditions. Plants treated with Asahi SL were higher and more advanced in development, particularly generative. Biomass accumulation was greater after biostimulator application mainly due to better photosynthetic apparatus efficiency, which was manifested by (i) greater leaf area, (ii) higher total chlorophyll content and (iii) increased intensity of photosynthesis. Effect of Asahi SL on chlorophyll a fluorescence was marginal. Despite of higher transpiration and lowered stomatal resistance the RWC was almost unchanged in biostimulator treated plants what was attributed to increased water uptake. Obtained results clearly showed that Asahi SL applied on plants can also be effective and beneficial when they are grown under optimal conditions.

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Zmiany klimatyczne Polski w latach 1983-2002 a perspektywy uprawy ziemniaka

67%

Boguszewska D.

Ziemniak Polski

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2006

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tom 16

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