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The quality of oocytes and embryos produced in vitro – the porcine and bovine model

100%

Cieslak D., Madeja Z., Orsztynowicz M., Pawlak P., Pers-Kamczyc E., Warzych-Plejer E.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2015

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tom 96

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nr 1

2

Embryo transfer as an option to improve fertility in repeat breeder dairy cows

84%

Nowicki A.

Journal of Veterinary Research

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2021

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tom 65

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nr 2

3

Biotechnological and clinical outcome of in vitro fertilization in non-obese patients with polycystic ovarian syndrome

84%

Ciepiela P., Baczkowski T., Brelik P., Antonowicz A., Safranow K., Kurzawa R.

Folia Histochemica et Cytobiologica. Supplement

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2007

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tom 45

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nr 1

65-71

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Association between the number and quality of bitch COC's and selected donor factors

84%

Bukowska D., Kempisty B., Antosik P., Jackowska M., Wozna M., Lianeri M., Jaskowski J. M.

Medycyna Weterynaryjna

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2010

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tom 66

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nr 07

480-483

The aim of the present study was to investigate whether selected factors attributed to oocyte donors can affect the number and quality of canine cumulus-oocyte-complexes (COC). The following parameters were considered: female age and body mass, ovarian weight, presence of functional ovarian structures (eg. corpus luteum, visible follicles), and ovarian and uterine pathology. Altogether 10 077 COCs were collected, on average 125.6 per bitch. The number and quality of collected COCs was significantly affected by female age and the presence of functional ovarian structures. We found an increased total number of COCs in up to 8-month-old bitches as compared to up-to-3-year-old and up-to-7-year-old females (P < 0.001). Moreover, senile females produced more high quality COCs than the younger ones. However, the differences between those groups were not statistically significant (P = 0.469, P = 0.346). A higher number of COCs was collected from ovaries with a smooth surface (202.8) or with visible follicles (121.1), in comparison to ovaries with corpus luteum (97.6), bitches with pyometra and pathologies of the ovaries or the uterus (82.8). No influence of the bitch’s body mass and ovarian weight on the number of COCs was observed, although a higher number of oocytes was usually collected from the right ovary. The quantity and morphological quality of bitch COCs varied significantly among individual females in an age-dependent manner. The greater number of COCs, isolated from younger bitches, may be associated with a higher reproductive potential and hormonal activity of these females. The study suggests that the age affects the total number of collected COCs but has no influence on the quality of bitch oocytes.

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Analysis of oocyte quality in recombinant inbred mouse strains developed from KE and CBA strains that differ in fertilization efficiency

84%

Wabik-Sliz B., Golas A., Krzanowska H.

Journal of Applied Genetics

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2005

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tom 46

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nr 2

Recombinant inbred (RI) mouse strains were developed from reciprocal crosses between two inbred strains differing in the proportion of fertilized ova (CBA, 100%; KE, 77%), to analyse the underlying factors. A correlation (r = 0.83, P< 0.01) between fertilization efficiency within 22 RI strains and after mating RI females with KE males proved that oocyte quality was involved. The following oocyte parameters were analysed in RI and progenitor strains: time of meiotic maturation, rapidity of enzymatic removal of egg investments, and proportion of fertilized ova with supplementary spermatozoa in the perivitelline space. Among the RI strains, high incidence of supplementary spermatozoa was correlated with lower efficiency of fertilization (r = -0.58, P < 0.05) and with slow meiotic maturation (r=-64, P < 0.01), suggesting that delayed maturation may affect oocyte ability of being fertilized by the first penetrating spermatozoon. However, significant correlations were also found between characters which coexist within the progenitor strains, but are not likely to be physiologically related; this suggests that RI strains have inherited large blocks of progenitor genomes, not disrupted by recombination. The strain distribution pattern (SDP) of the analysed traits revealed CBA-like, KE-like, and intermediate phenotypes, indicating that they are polygenically determined. No linkages were found between the studied traits and 12 enzymatic markers. However, the SDP for fertilization efficiency showed a preponderance of non-matching strains (15/19) in relation to agouti locus; the known instability of this chromosome region makes it possible that a putative linkage was disrupted by recombination when RI strains were created.

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Role of oocyte quality in meiotic maturation and embryonic development

67%

Marteil G., Richard-Parpaillon L., Kubiak J. Z.

Reproductive Biology

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2009

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tom 09

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nr 3

7

Quality and number of cumulus-oocyte complexes (COC) and concentrations of leptin and ghrelin in blood and follicular fluid depending on the body condition of cows

67%

Nowak T., Jaskowski J., Szczepankiewicz D., Olechnowicz J.

Medycyna Weterynaryjna

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2011

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tom 67

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nr 02

The aim of our study was to investigate the effect of body condition on the number of ovarian follicles, the number and quality of COC-complexes and/or concentration of leptin (LEP), total and active ghrelin (TGHR and AGHR) in blood plasma and follicular fluids. Before slaughter all cows were divided into five classes according to their body condition scores (BCS). Body condition had no significant influence on the number of follicles and aspirated oocytes. The mean number of oocytes of a very good quality (Grade 1 and Grade 2) was 0.79 and 1.14 in cows with BCS 2.50 and 2.51-3.0, and was lower than 2.69, 2.66 and 2.0, respectively, aspirated from follicles in cows from group 3.01-3.5, 3.51-4.0 and > 4.0. Body condition influenced the number of COCs of a very good quality (Grade 1, P < 0.05). There was no relation between body condition and the number of oocytes grades 2 and 4. We found an interrelation between blood leptin concentration and its concentration in the follicular fluid (P < 0.01). No significant relationship exists between the BCS of cows and concentrations of leptin and active ghrelin in blood plasma and the follicular fluid.