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‘Žutica’ is the most widespread olive variety of Montenegro, accounting for as much as 98% of olive trees in the southern part of Montenegrin coastal area – Bar subarea. The primary purpose of variety ‘Žutica’ is olive oil production due to its small fruits and high oil content, although it is also much appreciated as a table olive, prepared in local ways as green and black. As ‘Žutica’ is an old olive variety there are some phenotypic differences recorded in the fruit properties. Here we evaluated 22 accessions from the area of Bar and Ulcinj, where this variety is very widespread (almost mono-varietal), for 34 parameters of the fruit and endocarp. Significant differences in fruit properties were observed in the accessions. Average fruit size in nine accessions was above 3.5 g and in two accessions more than 4.0 g. Number of fruits per kg ranged from 224 to 330. Cluster analysis and principal component analysis (PCA) were used to group the accessions and evaluate the morphological variability. The accessions clustered into two groups, with two off group accessions of the highest fruit weight. The results showed differences among individuals, especially for large fruit accessions that should be investigated further.
Water is the most important environmental factor in growth and fruit yield of trees. To study the effect of deficit irrigation on reproductive characteristics and yield of seven superior olive genotypes of D1, Dd1, Gw, Ps1, Bn3, Bn6, and Ds17. The present research was accomplished in Dallaho Olive Research Station of Sarpol-e zahab, Kermanshah, Iran, in 2014 and 2015. Seven superior olive genotypes were studied in a randomized complete block design with three replicates and three irrigation regimes. The irrigation treatments included: 100% full irrigation (control), 75% deficit irrigation, and 50% deficit irrigation applied during the growth season. The results indicated that the genotypes had different reactions to the deficit irrigation regimes. Dd1 had the highest fruit weight, while the lowest fruit weight was observed in Ps1 and Gw. The highest fruit yield was found in Bn3, Bn6, and Dd1, while the lowest was observed in Ps1. As a result, Bn6 and Dd1 are introduced as the genotypes that are resistant to drought in the field.
The Mediterranean Region is the major area devoted to olive tree crop, and therefore a study of olive flowering is of great interest for the European Community. On the other hand, olive pollen is one of the main causes of pollen allergy in the Mediterranean area. Olive flowering is affected by climatic factors such as temperature and photoperiod, which vary geographically in latitude and altitude. Temperature has been used to study those processes that lead to flowering in the olive tree. The aim of the present paper is firstly the comparison of the flowering full bloom dates in two Mediterranean areas, Sicily (Italy) and Cordoba (Spain), located in the same latitudinal band (37-38oN) and to calculate the heat requirement until flowering by determination of different threshold temperatures and methods of heat accumulation. A delay of the full flowering dates in the Spanish compared with the Italian olive groves was observed. The most suitable threshold temperatures were carried out in a 7-15oC range by considering the heat accumulation start on 1 January in each olive grove. In particular, some causes were indicated as responsible for the different threshold temperatures recorded in the 2 study areas: First, the different climatic conditions (continental and insular climate) secondly the different cultivars present in the olive groves.
To tolerate harsh climatic conditions, olive tree Chetoui has developed some anatomic, physiologic and biochemical mechanisms. The aim of this study was to determine the indicators of stress in leaves, stems and roots growing under various climatic conditions. To protect against stress conditions this cultivar increased cuticle thickness, protective structures and building parenchyma tissues of leaves, woods and roots from the North to the South. The volatile compounds, extracted from northern and southern Chetoui leaves and roots, were analyzed by GC-FID and GC-MS. Great changes in volatiles were illustrated in the studied organs, by enrichment in phenolics and fatty acids for leaves and in hydrocarbons for roots of southern Chetoui. Also, a reduction in terpenes, alcohols and carbonylic compounds was noted in both southern samples. Moreover, minerals of all organs of Chetoui, varied in content and allocation, but their levels are the highest in leaves. The changes in volatiles might be affected by changes in the mineral elements uptake or accumulation under environment stress. A significant correlation was noted between phenolic compounds and sodium, nitrogen, and calcium contents. However, terpenoids was highly correlated with phosphorus content for all organs and studied areas. The detection of new volatiles, anatomical and mineral changes seem to be efficient indicators of adaptation of Chetoui to environment stress conditions.
Wildolive seeds (Olea europaea L. var. sylvestris), calledoleaster do not germinate when placedunder favourable conditions. In a series of experiments the effects of the harvest date, the endosperm, the tegument, andthe coldtreatment were evaluatedon germination of seeds andembryos. The germination percentage of embryos and seeds harvested at different harvest dates increased during October month, these percentages decreased during November month, whereas no seeds and embryos harvested on the middle of December germinated. Embryo germinability was always higher than seed germinability, and this may be due to an inhibiting effect of the teguments and the endosperm on seed germination. Such dormancy, which gradually increased during maturation, could reside mainly in the endosperm and partly within the embryo. The cold treatment at 4°C for four to thirteen days increased seed and embryo germinability, whereas lengthening time at this temperature showeda negative effect on seedgermination. The germination of seeds andembryos from six wildolive trees was also examinedby recording the germination percentage andminimum imbibition time (Tmi).
This paper describes the distribution of Ole e 3 allergen and its transcripts in the developing anther and in mature olive pollen. Northern blot and RT-PCR analyses over the course of pollen development show that Ole e 3 transcripts accumulate exclusively in mature pollen. This gene therefore corresponds to a "late gene." The sequences amplified by RT-PCR display high identity with those already reported for Ole e 3, including two Ca2+-binding motifs. Immunoblot analysis of the allergen shows that Ole e 3 accumulates during the same stage as the corresponding transcripts, suggesting a transcriptional regulation mechanism for the expression of the gene. Finally, the use of transmission electron microscopy techniques has shown that (a) the allergen is located mainly in the vicinity of membrane systems and in the aperture regions of the mature pollen grain, and (b) Ole e 3 transcripts are detectable after using in situ RT-PCR. These data are significant clues to the biological roles of the protein in olive pollen biology. Such putative functions are discussed.
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