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Effects of mismatches and insertions on discrimination accuracy of nucleic acid probes

100%

Piao X., Sun L., Zhang T., Gan Y., Guan Y.

Acta Biochimica Polonica

2008

tom 55

nr 4

713-720

Effective discrimination of non-complementary nucleotides is an important factor to ensure the accuracy of hybridization-based nucleic acid analyses. The current study investigates the effects of the chemical nature, the positions, the numbers, and the cooperative behavior of mismatches as well as insertions on 20-mer and 30-mer duplexes. We observed the hybridization stability trend affected by mismatches: G:T ≈ G:G > G:A > A:A ≈ T:T > A:C ≈ T:C > C:C. The experimental data show that mismatches at the center of the oligonucleotide probes have a more profound destabilizing effect on the hybridization stability than those at either ends. Insertions also demonstrate a similar destabilizing effect as mismatches. These results provide useful information for designing DNA microarray nucleotide probes and for improving the discrimination accuracy of hybridization-based detections.

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Transcriptome sequencing: next generation approach to RNA functional analysis

67%

Zmienko A., Jackowiak P., Figlerowicz M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

2011

tom 92

nr 4

Role of hydrophobic amino acids at position 74 of DRB1 chain in rheumatoid arthritis

67%

Cerna M., Havelka S., Ivaskova E.

Archivum Immunologiae et Therapiae Experimentalis

1995

tom 43

nr 2

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Archivum Immunologiae et Therapiae Experimentalis

1 BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

1 Cerna M.

1 Figlerowicz M.

1 Gan Y.

1 Guan Y.

1 Havelka S.

1 Ivaskova E.

1 Jackowiak P.

1 Piao X.

1 Sun L.

1 Zhang T.

1 Zmienko A.

1 2011

1 2008

1 1995

Wyniki wyszukiwania

Effects of mismatches and insertions on discrimination accuracy of nucleic acid probes

Transcriptome sequencing: next generation approach to RNA functional analysis

Role of hydrophobic amino acids at position 74 of DRB1 chain in rheumatoid arthritis