It has been investigated, with the aid of routine cytochemical methods, whether any changes of the activity of oxydoreductases of G. candidum which has been intluenced by chemical factors affecting its growth, and with which it can contact in human organism or his environment, occur. The factors were: antibacterial antibiotic from tetracycline group /chlortetracycline/, antifungal antibiotic representing polyenes derivatives /nystatine/, heavy metal salt /mercuric chloride/, aniline dye /malachite green/. To evaluate cytochemical preparates in which the fungus ce lis have been classified according to intensity of enzymatic reaction the chi² test has been suggested. It has been proved that all the investigated compounds have significant changes in the fungus metabolism showing in the oxydoreductases activity deviations.
Changes in the structure enzyme proteins occurring under high pressure can in some cases limit the usefulness of the high pressure technique in the preservation or mild processing of food. However, other changes can be advantageous in forming functional properties of food products. High pressure can affect the activity of proteolytic enzymes of meat by changing their conformation or releasing organelles from the cell and through the increase in concentration of activators or inhibitors in a reaction medium. Changes in enzyme activity, mainly of cathepsins and calpains, caused by high pressure affect the texture of meat. The final effect depends on many factors, primarily on the parameters of pressurization and the post mortem state of meat. The enzymes of coldwater fish are more sensitive to high pressure than their counterparts present in mammal meat or fish living in warm waters. The high pressure causing partial inactivation of oxydoreductases and lipolytic enzymes prevents the deterioration of the sensory quality of the product.