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The studies on the development of queen bees experimentally infected with females of V. jacobsoni were carried out in 1983 and 1984. Fully formed queen cells were transferred from the hive to the laboratory. At the base of each cell, except for the control group (K 1) a 3 mm opening was made through which females of V. jacobsoni were inserted, collected from brood drone. One female of the mite was inserted into each queen cell for the experimental group I, two - of the group II, three - group III and four - group IV. The openings in the cells were then sealed with warm wax, and the cells were placed in Zander containers and in a thermostat, at the temperature of 34.5°C, and relative humidity ca. 80%. Unopened queen cells constituted the control group (K 1), as well as cells opened and then sealed with wax (K 2). Of 508 control and experimental queen cells, 234 queens emerged (46.1%), of these 90 (88.9%) of a wight 189.8 mg in the group K 1, 72 (86.7%) of weight 189.5 mg in the group K 2, 81 (75.7%) of weight 188.2 mg in the group I, 48 (55.2%) of weight 184.6 mg in the group II, 23 (31.5%) of weight 189.1 mg in the group III and 20 (29.4%) of weight 192.4 mg in the grup IV. The remaining queens died at the stage of straightened larva (99), prepupa (17) and pupa (36). The highest number of queens died in the experimental groups II-IV. The results obtained indicate that the development cycle of V. jacobsoni can not be completed in queen bee cells.
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Profesor zw. dr hab. Bernard Bezubik [1919-2006]

80%
Muscles of the bream Abramis brama (L.) infected and not infected with plerocercoids of L. intestinalis, as well as the plerocercoids constituted the material. In the studied material the content of water, proteins (biuret method), hydrocarbons (antron method) and lipids (Folch's method), as well as the qualitative lipid composition (thin layer chromatography on silicagel) were determined. The muscles of infected fish showed a slightly higher (by 1.6% fresh weight) water content and a slightly lower content of proteins, hydrocarbons and lipids (by 0.6%, 0.03% and 0.6°/o respectively). In the body of the plerocercoids the content of water and proteins was lower than in the host muscles (by 11% and 8% respectively), as well as that of lipids (by 2.6% and 2.7%), while the hydrocarbon content was much higher (16.7% for the plerocercoids, 0.06% for the host). It is interesting to note the high phospholipid content (31.2°/o) in the lipid pool of the plerocercoids, along with the simultaneous decrease of this fraction in the respective pool of infected bream. Besides, in the group of neutral lipids of the muscular tissue of the bream triglycerides form a significant part. The slight changes in the chemical composition of the muscles of infected fish, as compared with the relatively high level of their energy reserves (as triglycerides), suggest that the negative effect of the parasite on the physiological condition of its host is rather limited.
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