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Bovine chlamydophilosis monitoring programs have not been run in Poland during the last decade, therefore the epidemiological status of the disease remains unrecognised. Recently observed animal movements, including international trade and import of livestock into Polish territory, support the necessity of serological surveillance of chlamydophilosis in representative subsets of the Polish bovine population. The aim of the study was the evaluation of Chlamydophila psittaci and Chlamydophila abortus seroprevalence in a subset of the bovine population and the comparative evaluation of serological techniques used in chlamydophilosis diagnostics (CFT, ELISA).
Medycyna Weterynaryjna
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2010
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tom 66
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nr 11
s.728-731,bibliogr.
The paper presents the most recent data and opinions concerning Brucella (B.) suis and porcine brucellosis. Included are up-to-date results concerning the classification of the genus Brucella into 9 species, among them B. suis. Five biovars of this species are mentioned. Biovars 1, 2 and 3 (particularly 1 and 3) are highly pathogenic for swine and for humans. The source of infection for swine are, besides swine, wild boar and hare. The global distribution of swine brucellosis is presented with particular reference to the European Union member countries. Since at present with the closed system swine production in the EU B. suis infection is not diagnosed, the main goal of veterinary services is the complex action against the introduction of B. suis into the national swine herds. The main risk factors are: importing of infected pigs from abroad and transmission of B. suis from the wild boar or the hare to the domestic swine. In order to exclude these possibilities, besides available directives and instructions, diagnostic laboratory investigations are of crucial importance. In the paper direct and indirect tests for the identification of B. suis infection are described and assessed with respect to their diagnostic value. Concerning direct diagnostic tests, besides traditional assays based on phenotypic properties of the microorganism, several genotypic methods and among them particularly the polymerase chain reaction (PCR) are characterized. Following this, indirect diagnostic methods, being serological tests used in the diagnosis of porcine brucellosis, are discussed. Their diagnostic value is evaluated, including the possibility of false positive and false negative reactions. The Brucellin allergic skin test, as a confirmatory test of the serological results, is presented. In conclusion it is stated that the present epizootic situation concerning porcine brucellosis in the EU member countries is satisfactory. In connection with this the importance of prophylactic activity of the veterinary services against transmission of B. suis infection to domestic pigs of this region is stressed.
The article concentrates on diagnostic aspects of the major mycoplasma diseases in ruminants. Among them particular attention is given to: bovine contagious pleuropneumonia and contagious agalactia of sheep and goats, and an involvement of mycoplasmal etiological agent in bovine respiratory disease (BRD). The diagnosis of mycoplasma infections is based on serological methods such as: Elisa test, complement fixation test (CFT), and Western blot. The culture and identification of Mycoplasma spp. are possible through the use of specific liquid or solid Eaton’s media. The doubtful cases are confirmed by molecular biology methods (PCR and DGGE).
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