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Modulation of some nuclear matrix enzymatic activities by free fatty acids

100%

Lubocka J., Szopa J., Kozubek A.

Acta Biochimica Polonica

1995

tom 42

nr 2

It was shown that two of main enzymatic activities of plant nucleus and nuclear matrix, namely RNA-polymerasic and DNA-nucleolytic are susceptible to modulation with free fatty acids. The effects observed were dependent to both fatty acid length and degree of unsaturation. In nuclei a stimulation of nuclease activity was observed whereas in matrices short chain fatty acids inhibited the studied activity. The effect of fatty acids on RNA-poIymerase was also different in nuclei and matrices. In nuclei all fatty acids studied inhibited polymerasic activity whereas in matrices short chain fatty acids stimulated this activity by up to 80% and the long chain fatty acids inhibited by up over 70%. The overall alteration of studied activities in nuclei and matrices by unsaturated fatty acids was similar. Nucleolytic activity was stronger inhibited and polymerasic activity was stimulated when the effects of linoleic and linolenic acids were studied. The results suggest possible importance of lipid component in nuclear matrix biological function.

Modification of the lipid component modulates nuclear matrix nucleolytic activity

100%

Lubocka J., Szopa J., Kozubek A.

Acta Biochimica Polonica

1995

tom 42

nr 2

It was shown that lipid composition of plant nuclear matrix depends on procedure of its isolation. The matrix isolated with the use of lithium diodosalicylate (LiS) differs in its lipid composition from the preparation isolated with the use of nonionic detergent (Triton X-100). It was also shown that the nucleolytic activity of the matrix is related to its lipid component. Matrix depleted in lipids loses half of its nucleolytic activity which is recovered after supplementation with previously extracted lipids. The extent of recovery of the nucleolytic activity is also dependent on the presence of residual DNA in matrix preparation. The recoveries of nucleolytic activities were higher in matrices not depleted in their DNA content.

Ograniczanie wyników

2 Acta Biochimica Polonica

2 Kozubek A.

2 Lubocka J.

2 Szopa J.

2 1995

Wyniki wyszukiwania

Modulation of some nuclear matrix enzymatic activities by free fatty acids

Modification of the lipid component modulates nuclear matrix nucleolytic activity