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The 14-3-3 protein binds to the nuclear matrix endonuclease and has a possible function in the control of plant senescence

100%

Markiewicz E., Wilczynski G., Rzepecki R., Kulma A., Szopa J.

Cellular and Molecular Biology Letters

1996

tom 01

nr 4

The nuclear matrices of plant cell nuclei display an intrinsic nuclease activity which is capable of nicking supercoiled DNA. Recently a cDNA encoding the 14-3-3 protein from Cucurbita pepo has been cloned and sequenced. The evidence that the recombinant 14-3-3 protein associates with DNase I and endogenous plant nuclease is presented. Evidence is also presented that the cloned 14-3-3 protein isoform, unique in its binding to nuclease within the 14-3-3 family, is located in the nuclei and in the nuclear matrix. Transgenic potato plants were created where the 14-3-3 protein derived from Cucurbita was overexpressed. An increase in tuber number and a decrease in tuber size in the transformants was also observed. The adenine nucleotide pool in leaves of transgenic plants was significantly reduced and they contain more chlorophyll and loose it slower when grown in the dark. A decrease in 14-3-3 protein content concomitant with an increase in nuclease activity in senescent plants was detected and this was markedly delayed in transgenic potato plants which overexpressed the 14-3-3 protein. It is proposed that a function of the isolated 14-3-3 isoform is the control of the nuclease activity and hence senescence.

Modulation of plant nuclear matrix nucleolytic activity and specificity may result from interaction of nuclease-protein 14-3-3 complex with inner nuclear membrane lipids

100%

Lubocka J., Kozubek A.

Cellular and Molecular Biology Letters

1998

tom 03

nr 1

Plant cell nuclear matrix depleted in lipids exhibited approximately 50% lower endonucleolytic activity attributed to 32 kDa nuclease. Supplementation of delipidated matrix with phospholipids, sterols and glycolipids resulted in the recovery of nuclease activity. The extent of recovery is dependent upon the type of lipid used for reconstitution. The most effective (over 97%) in recovery of the nucleolytic activity were PC, PE, DGDG and stigmasterol. Some recovery is also observed when other natural amphiphilic molecules are studied (resorcinolic lipids). Recombinant plant protein 14-3-3 showed ability for direct interaction with lipid monolayers. The interaction was confirmed by analysis of the monolayer collected from the subphase after incubation with protein 14-3-3. Deletion of the 12 kDa N-terminal fragment of the protein 14-3-3 abolished its ability for binding to lipid monolayer. The results suggest that in vivo direct interaction of protein 14-3-3 with nuclear envelope lipids may participate in modulation of the nuclease 32 kDa activity. A postulated model of the interactions is discussed.

Ograniczanie wyników

2 Cellular and Molecular Biology Letters

1 Kozubek A.

1 Kulma A.

1 Lubocka J.

1 Markiewicz E.

1 Rzepecki R.

1 Szopa J.

1 Wilczynski G.

1 1998

1 1996

Wyniki wyszukiwania

The 14-3-3 protein binds to the nuclear matrix endonuclease and has a possible function in the control of plant senescence

Modulation of plant nuclear matrix nucleolytic activity and specificity may result from interaction of nuclease-protein 14-3-3 complex with inner nuclear membrane lipids