Wyniki wyszukiwania

1

The role of the NUR77 family of nuclear receptors in the signalling pathways of thymocytes and neurons

100%

Matuszyk J.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

2

Nuclear receptors, their coactivators and modulation of transcription

75%

Manteuffel-Cymborowska M.

Acta Biochimica Polonica

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1999

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tom 46

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nr 1

Nuclear receptors arc ligand-dependent transcription factors which can also be activated in the absence of their lipophilic ligands by signaling substances acting on cell membrane receptors. This ligand-independent activation indicates the importance of nuclear receptor phosphorylation for their function. Nuclear receptor- mediated transcription of target genes is further increased by interactions with recruited coactivators forming a novel family of nuclear proteins. CBP/p300, a coactivator of different classes of transcription factors, including the tumor suppressor protein p53, plays a special role acting as a bridging protein between inducible transcription factors and the basal transcription apparatus, and as an integrator of diverse signaling pathways. Coactivators of nuclear receptors and associated proteins forming a multicomponent complex have an intrinsic histone acetylase activity in contrast to nuclear receptor and heterodimer Mad-Max corepressors, which recruit histone deacetylase. Similarly the Rb protein interacts with histone deacetylase to repress transcription of cell cycle regulatory genes. Targeted histone acetylation/dca- cetylation results in remodeling of chromatin structure and correlates with activation/repression of transcription. Recent data point to the important role of coactivator proteins associated with inducible transcription factors in transcription regulation, and in the integration of multiple signal transduction pathways within the nucleus.

3

Liver X receptor agonist T0901317 enhanced peroxisome proliferator-activated receptor-delta expression and fatty acid oxidation in rat skeletal muscle

63%

Baranowski M., Blachnio-Zabielska A. U., Zabielski P., Harasim E., Harasiuk D., Chabowski A., Gorski J.

Journal of Physiology and Pharmacology

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2013

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tom 64

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nr 3

4

The impact of Di(2-ethylhexyl)phthalate on cancer progression

63%

Chou C.-K., Yang Y.-T., Yang H.-C., Liang S.-S., Wang T.-N., Kuo P.-L., Wang H.-M. D., Tsai E.-M., Chiu C.-C.

Archivum Immunologiae et Therapiae Experimentalis

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2018

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tom 66

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nr 3

5

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Recent advances in computational chemistry for identification of ligands for biological receptors: interdisciplinary aspects

63%

Todorov M.

Medical Science Pulse

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2018

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tom 12

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nr 1

Background: Computational (in silico) methods, such as quantitative structure-activity relationships (QSARs) are already well recognized and used in many screening programs related to environmental, industrial and medical chemistry. The main idea of the QSAR is that there is a relationship between molecular structure and ultimate biological effect caused by a chemical compound. In this respect the approach could be used successfully for prediction of various biological endpoints caused by chemical compounds including receptor binding affinity. Aim of the study: In the current study the capabilities for structure-activity modelling incorporated in noncommercial software tool have been employed for investigating the binding effect of xenobiotics toward estrogen and human pregnane X receptor. Material and methods: The analysis was performed by making use of the non-commercial software platform QSAR Toolbox. This system allows application of a set of built-in models for different biological effects, and also allows incorporation of new models for other endpoints. Results: Two models have been applied for predicting the binding effect toward estrogen and human pregnane X receptors of a large number of chemicals collected in a single database of high practical concern. The results show that there are many chemicals which are able to bind the investigated receptors. Since those chemicals are encountered in the environment, they could be considered as potential threat for society. Conclusions: The obtained results could be used as initial step for further experimental testing of those chemicals in order to confirm their potential to harm biological systems in the body.

6

Gonadotropin-releasing hormone and kisspeptin-10 regulate nuclear receptor subfamily 5 group a member 1/catenin beta 1/nuclear receptor subfamily 0 group B member 1 activity in female rat anterior pituitary gland

63%

Zielinska-Gorska M., Gorski K., Biernacka K., Sawosz E., Kaminski T., Gajewska A.

Journal of Physiology and Pharmacology

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2018

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tom 69

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nr 3

7

LXR regulate cholesterol homeostasis in the proximal mouse epididymis

63%

Ouvrier A., Cadet R., Lobaccaro J.-M. A., Drevet J. R., Saez F.

Folia Histochemica et Cytobiologica

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2009

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tom 47

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nr 5

8

Gene expression profile of estrogen receptor alpha and beta in the ovaries of Zi geese (Anser cygnoides)

63%

Kang B., Jiang D. M., Liu B., Zhou R. J., Zhen L., Yang H. M.

Folia Biologica

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2011

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tom 59

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nr 3-4

9

Degradation and beyond: control of androgen receptor activity by the proteasome system

63%

Jaworski T.

Cellular and Molecular Biology Letters

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2006

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tom 11

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nr 1

The androgen receptor (AR) is a transcription factor belonging to the family of nuclear receptors which mediates the action of androgens in the development of urogenital structures. AR expression is regulated post-translationally by the ubiquitin/proteasome system. This regulation involves more complex mechanisms than typical degradation. The ubiquitin/proteasome system may regulate AR via mechanisms that do not engage in receptor turnover. Given the critical role of AR in sexual development, this complex regulation is especially important. Deregulation of AR signalling may be a causal factor in prostate cancer development. AR is the main target in prostate cancer therapies. Due to the critical role of the ubiquitin/proteasome system in AR regulation, current research suggests that targeting AR degradation is a promising approach.

10

Functionality versus strength - has functional selection taken place in the case of the ecdysteroid receptor response element?

63%

Grad I., Kochman M., Ozyhar A.

Acta Biochimica Polonica

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2002

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tom 49

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nr 3

Nuclear receptors are ligand-dependent transcription factors responsible for controlling differentiation, growth and development of higher eukaryotes. Three amino acids within the recognition a-helix of the DNA-binding domain of the nuclear receptors constitute the so-called "P-box" which determines response element specificity. In the ultraspiracle (Usp) protein, which together with EcR forms the heterodimeric ecdysone receptor, the P-box residues are E19, G20 and G23. Substitution of E19, the most characteristic amino acid for estrogen receptor-like P-boxes, with alanine showed that the mutation did not appreciably alter the affinity of the wild-type Usp DNA-binding domain (UspDBDwT for a probe containing natural ecdysone response element (hsp27wt). Since in many cases E19 contacts a G/C base pair in position -4, which is absent in hsp27wt, we analysed the interaction of UspDBDwT, E19A and other P-box region mutants with the hsp27wt derivative which contains a G/C instead of an T/A base pair in position -4. UspDBDwT exhibited higher affinity for this element than for hsp27wt. Moreover, a different interaction pattern of P-box region mutants was also observed. Thus we conclude that the E19 residue of UspDBD is not involved in any hsp27wt sequence-discerning contacts. However, substitution of the hsp27wt T/A base pair in position –4 with G/C generates target sequence with distinct functional characteristics and possibly with a new specificity. These results could serve as a basis for understanding the role of the presence of a T/A or G/C base-pair in the position –4 in the two types of ecdysone response elements found in nature.

11

Thyroid hormone receptor alpha1: a switch to cardiac cell 'metamorphosis'?

63%

Pantos C., Xinaris C., Mourouzis I., Perimenis P., Politi E., Spanou D., Cokkinos D. V.

Journal of Physiology and Pharmacology

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2008

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tom 59

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nr 2

Thyroid hormone receptor alphalpha1 (TRalpha1) is predominantly expressed in the myocardium but its biological function under physiological or pathological conditions remains largely unknown. The present study investigated possible interactions between alpha1 adrenergic and thyroid hormone signaling at the level of TRalpha1, potential underlying mechanisms and physiological consequences, as well as the role of TRalpha1 in cell differentiation. This may be of physiological relevance since both thyroid hormone and adrenergic signalling are implicated in the pathophysiology of cardiac remodelling. Neonatal cardiomyocytes obtained from newborn rats (2-3 days) were exposed to phenylephrine (PE, an alpha1 adrenergic agonist) for 5 days, in the absence or excess of T3 in the culture medium. PE, in the absence of T3, resulted in 5.0 fold increase in TRalpha1 expression in nucleus and 2.0 fold decrease in TRalpha1 expression in cytosol, P<0.05. As a result, a fetal pattern of myosin isoform expression with marked expression of ß–MHC was observed in PE treated vs the untreated cells, P<0.05. PD98059 (an ERK signalling inhibitor) abrogated this response. In the presence of T3 in the culture medium, TRalpha1 expression was increased 1.6 fold in nucleus and 2.0 fold in cytosol in PE-T3 vs PE treated cells, P<0.05, and the fetal pattern of myosin isoform expression was prevented. Parallel studies with H9c2 myoblasts showed that reduction of T3 binding to TRalpha1 receptor delayed cardiac myoblasts differentiation without affecting proliferation. In conclusion, in neonatal cardiomyocytes, nuclear TRalpha1 is overexpressed after prolonged activation of the alpha1- adrenergic signalling by PE. This response seems to be an ERK kinase dependent process. Over-expression of TRalpha1 may lead to fetal cardiac phenotype in the absence of thyroid hormone availability. Furthermore, TRalpha1 seems to be critical in cardiac myoblast differentiation.

12

Short term 13-cis-retinoic acid treatment at therapeutic doses elevates expression of leptin, GLUT4, PPARgamma and aP2 in rat adipose tissue

51%

Krskova-Tybitanclova K., Macejova D., Brtko J., Baculikova M., Krizanova O., Zorad S.

Journal of Physiology and Pharmacology

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2008

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tom 59

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nr 4

731-743

Temporary defects in the plasma lipid and glucose homeostasis are frequent complication accompanying chronic treatment with 13-cis-retinoic acid (13cRA). White adipose tissue acts as an endocrine organ producing a variety of hormones (adipocytokines) including leptin, adiponectin, tumor-necrosis factor alpha (TNF) and angiotensin II (Ang II), which influence lipid metabolism, systemic insulin sensitivity and inflammation. To study the effect of a short-term 13cRA administration on metabolism of epididymal fat tissue, we treated Wistar rats with five identical therapeutic doses of 13cRA (0.8 mg/kg b.w.) by gavage during a period of 10 days. Expression of adiponectin, leptin, TNF and selected proteins such as adipocyte fatty acid binding protein (aP2), insulin-dependent glucose transporter GLUT4, peroxisome proliferator-activated receptor gamma (PPAR) and retinoid X receptors (RXRs) was investigated using RT-PCR. Short-term treatment with therapeutic doses of 13cRA caused significant increase of the aP2, PPAR and moderately RXR gene expression. Similarly, the relative amount of mRNA for leptin and GLUT4 was increased, while the TNF transcript was decreased after treatment with 13cRA. The gene expression and plasma concentration of adiponectin were without any significant changes. Since local adipose renin-angiotensin system (RAS) has been presumed to be involved in the regulation of fat tissue metabolism, we also investigated the gene expression of RAS components in epididymal fat depot. Our data has shown that 13cRA elevated Ang II receptor type 1 (AT1 receptor) - at both, mRNA and protein level. Thus, our results demonstrate that short-term 13cRA treatment is inducing alterations in fat tissue metabolism in relation to stimulated adipogenesis.

13

Rola receptorow jadrowych w indukcji form molekularnych cytochromu P-450 pod wplywem substancji obcych

51%

Palut D., Kostka G., Strucinski P.

Roczniki Państwowego Zakładu Higieny

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2002

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tom 53

|

nr 4

321-332

Omówiono mechanizmy leżące u podstaw indukcji podrodziny CYP2B, ЗА i 4A oraz udział w tych procesach heterodimerycznych receptorów jądrowych.

14

Membrane estrogen receptors - is it an alternative way of estrogen action?

51%

Soltysik K., Czekaj P.

Journal of Physiology and Pharmacology

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2013

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tom 64

|

nr 2

15

Influence of 17beta-estradiol on survival and proliferation of HeLa line cells

51%

Dziubinska-Parol I., Rzymowska J., Myga M., Parol W., Gozdzicka-Jozefiak A., Kwasniewska A.

Polish Journal of Environmental Studies

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2004

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tom 13

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nr Suppl.2, Part 1

Objectives: Molecular mechanism of carcinogenesis associated with high risk (HR) type HPV is related to the activity of virus oncoproteins E5, E6 and E7. Their task is to bring the cells to a state enabling synthesis of viral DNA, copying viral particles and promotion of uncontrolled cell growth. Proliferative factors of a feminine genital tract epithelium are also sex hormones — estradiol and progesterone. Steroids influence the transcription of genes involved in cell cycle, acting through specific nuclear receptors (ER and PR). The aim of this study was evaluation of the effect of selected concentrations of 17ß-estradiol and progesterone on survival and proliferation of HeLa line cells. Material and methods: The study was done on a transformed HeLa cell line containing integrated genome HPV of type 18. The lines were incubated in the presence of 17ß-estradiol at the concentrations of lx10-4M, lx10-5M, lx10-7M, lx10-8M. Cell survival was determined with the use of 0.5% of water solution of toluidine blue and the cell proliferation rate were evaluated with the use of BrdU Labelling and Detection Kit and the method ELISA (Roche). Results: The obtained results point to 10% toxic influence on HeLa line cells of 17ß-estradiol at high concentrations after 72 h of incubation. The influence of the hormone on proliferation rate was diversified depending on the hormone concentration and time.

16

Nuclear progesterone receptor isoforms and their functions in the female reproductive tract

51%

Rekawiecki R., Kowalik M., Kotwica J.

Polish Journal of Veterinary Sciences

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2011

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tom 14

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nr 1

Progesterone (P4), which is produced by the corpus luteum (CL), creates proper conditions for the embryo implantation, its development, and ensures proper conditions for the duration of pregnancy. Besides the non-genomic activity of P4 on target cells, its main physiological effect is caused through genomic action by the progesterone nuclear receptor (PGR). This nuclear progesterone receptor occurs in two specific isoforms, PGRA and PGRB. PGRA isoform acts as an inhibitor of transcriptional action of PGRB. The inactive receptor is connected with chaperone proteins and attachment of P4 causes disconnection of chaperones and unveiling of DNA binding domain (DBD). After receptor dimerization in the cells’ nucleus and interaction with hormone response element (HRE), the receptor coactivators are connected and transcription is initiated. The ratio of these isoforms changes during the estrous cycle and reflects the different levels of P4 effect on the reproductive system. Both isoforms, PGRA and PGRB, also show a different response to the P4 receptor antagonist activity. Connection of the antagonist to PGRA can block PGRB, but acting through the PGRB isoform, P4 receptor antagonist may undergo conversion to a strongly receptor agonist. A third isoform, PGRC, has also been revealed. This isoform is the shortest and does not have transcriptional activity. Alternative splicing and insertion of additional exons may lead to the formation of different PGR isoforms. This paper summarizes the available data on the progesterone receptor isoforms and its regulatory action within the female reproductive system.

17

The quantitative expression of peroxisome proliferator activated receptor (PPAR) genes in porcine endometrium through the estrous cycle and early pregnancy

51%

Bogacka I., Bogacki M.

Journal of Physiology and Pharmacology

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2011

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tom 62

|

nr 5

18

Biological roles of liver X receptors in immune cells

51%

Pascual-Garcia M., Valledor A. F.

Archivum Immunologiae et Therapiae Experimentalis

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2012

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tom 60

|

nr 4

19

Steroid signal transduction activated at the cell membrane: from plants to animals

51%

Marcinkowska E., Wiedlocha A.

Acta Biochimica Polonica

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2002

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tom 49

|

nr 3

Steroid hormones in plants and in animals are very important for physiological and developmental regulation. In animals steroid hormones are recognized by nuclear receptors, which transcriptionally regulate specific target genes following binding of the ligand. In addition, numerous rapid effects generated by steroids appear to be mediated by a mechanism not depending on the activation of nuclear receptors. Although the existence of separate membrane receptors was postulated many years ago and hundreds of reports supporting this hypothesis have been published, no animal membrane steroid receptor has been cloned to date. Meanwhile, a plant steroid receptor from Arabidopsis thaliana has been identified and cloned. It is a transmembrane protein which specifically recognizes plant steroids (brassinosteroids) at the cell surface and has a serine/threonine protein kinase activity. It seems that plants have no intracellular steroid receptors, since there are no genes homologous to the family of animal nuclear steroid receptors in the genome of A. thaliana. Since the reason of the rapid responses to steroid hormones in animal cells still remains obscure we show in this article two possible explanations of this phenomenon. Using 1,25-dihydroxyvitamin D3 as an example of animal steroid hormone, we review results of our and of other groups concordant with the hypothesis of membrane steroid receptors. We also review the results of experiments performed with ovarian hormones, that led their authors to the hypothesis explaining rapid steroid actions without distinct membrane steroid receptors. Finally, examples of polypeptide growth factor that similarly to steroids exhibit a dual mode of action, activating not only cell surface receptors, but also intracellular targets, are discussed.

20

Molecular mechanisms of retinoid action

51%

Gronemeyer H., Miturski R.

Cellular and Molecular Biology Letters

|

2001

|

tom 06

|

nr 1

Ograniczanie wyników

The role of the NUR77 family of nuclear receptors in the signalling pathways of thymocytes and neurons

Nuclear receptors, their coactivators and modulation of transcription

Liver X receptor agonist T0901317 enhanced peroxisome proliferator-activated receptor-delta expression and fatty acid oxidation in rat skeletal muscle

The impact of Di(2-ethylhexyl)phthalate on cancer progression

Recent advances in computational chemistry for identification of ligands for biological receptors: interdisciplinary aspects

Gonadotropin-releasing hormone and kisspeptin-10 regulate nuclear receptor subfamily 5 group a member 1/catenin beta 1/nuclear receptor subfamily 0 group B member 1 activity in female rat anterior pituitary gland

LXR regulate cholesterol homeostasis in the proximal mouse epididymis

Gene expression profile of estrogen receptor alpha and beta in the ovaries of Zi geese (Anser cygnoides)

Degradation and beyond: control of androgen receptor activity by the proteasome system

Functionality versus strength - has functional selection taken place in the case of the ecdysteroid receptor response element?

Thyroid hormone receptor alpha1: a switch to cardiac cell 'metamorphosis'?

Short term 13-cis-retinoic acid treatment at therapeutic doses elevates expression of leptin, GLUT4, PPARgamma and aP2 in rat adipose tissue

Rola receptorow jadrowych w indukcji form molekularnych cytochromu P-450 pod wplywem substancji obcych

Membrane estrogen receptors - is it an alternative way of estrogen action?

Influence of 17beta-estradiol on survival and proliferation of HeLa line cells

Nuclear progesterone receptor isoforms and their functions in the female reproductive tract

The quantitative expression of peroxisome proliferator activated receptor (PPAR) genes in porcine endometrium through the estrous cycle and early pregnancy

Biological roles of liver X receptors in immune cells

Steroid signal transduction activated at the cell membrane: from plants to animals

Molecular mechanisms of retinoid action