Wyniki wyszukiwania

1

Organization of sequences homologous to tRNA in wheat nuclear genome

100%

Jarmolowski A., Szweykowska-Kulinska Z., Augustyniak J.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1988

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tom 36

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nr 7-9

2

A transcriptionally active copia-like retroelement in Citrus limon

100%

De F. B., Wilson R. R., Argenziano C., Kafantaris I., Conicella C.

Cellular and Molecular Biology Letters

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2009

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tom 14

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nr 2

289-304

The plant nuclear genome is largely composed of mobile DNA, which can rearrange genomes and other individual gene structure and also affect gene regulation through various promoted activities: transposition, insertion, excision, chromosome breakage, and ectopic recombination. Ty1-copia-like retrotransposon is a widespread class of transposable elements in the plant kingdom, representing a large part of the total DNA content. Here, a novel retrotransposon-like sequence was isolated and identified as the Ty1-copia-like reverse transcriptase domain (named here CLCoy1), based on the homology of known elements. Fluorescence in situ hybridization, revealed that CLCoy1 was mainly located in telomeric and sub-telomeric regions along the Citrus chromosomes. CLCoy1 composes 3.6% of the genome and, interestingly, while transposons are mostly specific to a species, this element was identified in other Citrus species such as Citrus aurantium, Fortunella margarita and Citrus paradisi, but undetected in Poncirus trifoliata. We also determined that wounding, salt and cell culture stress produced transcriptional activation of this novel retroelement in Citrus limon. The novel Ty1-copia-like element CLCoy1 may have played a major role in shaping genome structure and size during Citrus species evolution.

3

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Molecular characterization of nuclear and cytoplasmic genomes of Solanum × michoacanum (+) S. tuberosum somatic hybrids

84%

Smyda-Dajmund P., Sliwka J., Jakuczun H., Wasilewicz-Flis I., Zimnoch-Guzowska E.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2015

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tom 96

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nr 1

4

Short-term heat stress effects on a cyp 11A1 canola plants

84%

Sakhno L., Slyvets M., Korol N., Karbovska N., Ostapchuk A., Kuchuk M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 3

5

Involvement of plastid, mitochondrial and nuclear genomes in plant-to-plant horizontal gene transfer

84%

Sanchez-Puerta M. V.

Acta Societatis Botanicorum Poloniae

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2014

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tom 83

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nr 4

This review focuses on plant-to-plant horizontal gene transfer (HGT) involving the three DNA-containing cellular compartments. It highlights the great incidence of HGT in the mitochondrial genome (mtDNA) of angiosperms, the increasing number of examples in plant nuclear genomes, and the lack of any convincing evidence for HGT in the well-studied plastid genome of land plants. Most of the foreign mitochondrial genes are non-functional, generally found as pseudogenes in the recipient plant mtDNA that maintains its functional native genes. The few exceptions involve chimeric HGT, in which foreign and native copies recombine leading to a functional and single copy of the gene. Maintenance of foreign genes in plant mitochondria is probably the result of genetic drift, but a possible evolutionary advantage may be conferred through the generation of genetic diversity by gene conversion between native and foreign copies. Conversely, a few cases of nuclear HGT in plants involve functional transfers of novel genes that resulted in adaptive evolution. Direct cell-to-cell contact between plants (e.g. host-parasite relationships or natural grafting) facilitate the exchange of genetic material, in which HGT has been reported for both nuclear and mitochondrial genomes, and in the form of genomic DNA, instead of RNA. A thorough review of the literature indicates that HGT in mitochondrial and nuclear genomes of angiosperms is much more frequent than previously expected and that the evolutionary impact and mechanisms underlying plant-to-plant HGT remain to be uncovered.

6

The human genome structure and organization

84%

Makalowski W.

Acta Biochimica Polonica

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2001

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tom 48

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nr 3

Genetic information of human is encoded in two genomes: nuclear and mitochondrial. Both of them reflect molecular evolution of human starting from the beginning of life (about 4.5 billion years ago) until the origin of Homo sapiens species about 100000 years ago. From this reason human genome contains some features that are common for different groups of organisms and some features that are unique for Homo sapiens. 3.2 X 10 base pairs of human nuclear genome are packed into 23 chromosomes of different size. The smallest chromosome - 21st contains 5 X 10 7 base pairs while the biggest one -1st contains 2.63 X 10 8 base pairs. Despite the fact that the nucleotide sequence of all chromosomes is established, the organisation of nuclear genome put still questions: for example: the exact number of genes encoded by the human genome is still unknown giving estimations from 30 to 150 thousand genes. Coding sequences represent a few percent of human nuclear genome. The majority of the genome is represented by repetitive sequences (about 50%) and noncoding unique sequences. This part of the genome is frequently wrongly called "junk DNA". The distribution of genes on chromosomes is irregular, DNA fragments containing low percentage of GC pairs code lower number of genes than the fragments of high percentage of GC pairs.

7

Application of molecular techniques to taxonomic studies

67%

Lesicki A., Szweykowska-Kulinska Z.

Folia Malacologica

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1999

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tom 07

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nr 4

8

A comparison of PCR-based markers for the molecular identification of Sphagnum species of the section Acutifolia

67%

Sawicki J., Szczecinska M.

Acta Societatis Botanicorum Poloniae

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2011

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tom 80

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nr 3

RAPDs, ISJs, ISSRs, ITS and katGs were applied to determine genetic relationships between common Sphagnum species of the section Acutifolia. Twenty populations were genotyped using ten ISJ primers, 12 pairs of katG primers, 10 ISSR and 10 RAPD primers, and a restriction analysis of ITS1 and ITS2. ISSR and katG markers revealed the greatest number of species-specific bands. An analysis of ITS1 and ITS2 regions with restriction enzymes also proved to be a highly effective tool for species identification.

Ograniczanie wyników

Organization of sequences homologous to tRNA in wheat nuclear genome

A transcriptionally active copia-like retroelement in Citrus limon

Molecular characterization of nuclear and cytoplasmic genomes of Solanum × michoacanum (+) S. tuberosum somatic hybrids

Short-term heat stress effects on a cyp 11A1 canola plants

Involvement of plastid, mitochondrial and nuclear genomes in plant-to-plant horizontal gene transfer

The human genome structure and organization

Application of molecular techniques to taxonomic studies

A comparison of PCR-based markers for the molecular identification of Sphagnum species of the section Acutifolia