Wyniki wyszukiwania

1

Arginine methylation analysis of the splicing-associated SR protein SFRS9-SRP30C

100%

Bressan G. C., Moraes E. C., Monfiolli A. O., Kuniyoshi T. M., Passos D. O., Gomes M. D., Kobarg J.

Cellular and Molecular Biology Letters

|

2009

|

tom 14

|

nr 4

657-669

The human SFRS9/SRp30c belongs to the SR family of splicing regulators. Despite evidence that members of this protein family may be targeted by arginine methylation, this has yet to be experimentally addressed. In this study, we found that SFRS9 is a target for PRMT1-mediated arginine methylation in vitro, and that it is immunoprecipitated from HEK-293 lysates by antibodies that recognize both mono- and dimethylated arginines. We further observed that upon treatment with the methylation inhibitor Adox, the fluorescent EGFP-SFRS9 re-localizes to dot-like structures in the cell nucleus. In subsequent confocal analyses, we found that EGFP-SFRS9 localizes to nucleoli in Adox-treated cells. Our findings indicate the importance of arginine methylation for the subnuclear localization of SFRS9.

2

Nuclear bodies in paneth cells of the rat

100%

Sulikowska-Rowinska A., Rowinski J.

Folia Histochemica et Cytobiologica

|

1993

|

tom 31

|

nr 1

3

Viruses as hijackers of PML nuclear bodies

88%

Moller A., Schmitz M. L.

Archivum Immunologiae et Therapiae Experimentalis

|

2003

|

tom 51

|

nr 5

4

Nuclear bodies in hepatocytes of the rat

75%

Orkisz S., Bartel H., Tosik D.

Folia Histochemica et Cytobiologica. Supplement

|

1996

|

tom 34

|

nr 1

5

PML shuttles between nuclear bodies and the cytoplasm

63%

Stuurman N., Floore A., Middelkoop E., Van Driel R., De Jong L.

Cellular and Molecular Biology Letters

|

1997

|

tom 02

|

nr 2

Nuclear bodies are electron dense, spherical structures with a diameter between 0.2 and 1.0 µm. The function of these nuclear domains is unclear. One class of nuclear bodies contains the tumor suppressor protein PML. Besides in the nucleus, a small number of PML-containing structures of about the same size as nuclear bodies is also present in the cytoplasm. We have investigated whether PML is transported from the nucleus to the cytoplasm and/or vice versa. To this end we injected the PML-specific mAb 5E10 into the cytoplasm of living cells. Subsequently, we monitored translocation of the antibody across the nuclear envelope by indirect immunofluorescent microscopy. It is well known that antibodies in the cytoplasm of living cells do not enter the nucleus, unless as a complex with a karyophilic protein. We observed accumulation into PML-containing nuclear bodies of 5E10 microinjected into the cytoplasm. Control rabbit IgG and a mAb specific for lamin B2 were not translocated to the nucleus. All nuclear PML bodies were labeled simultaneously by 5E10 with gradually increased intensity in time. Labeling of PML-containing nuclear bodies by 5E10 microinjected into the cytoplasm was not affected by inhibition of protein synthesis. These results suggest that the 5E10 antigen PML shuttles between the nucleus and cytoplasm, indicating that nuclear bodies are dynamic structures.

6

Ultrastructural components of the nuclei of undifferentiated crypt base cells in the human small intestine - stereological of volumes

63%

Wilczynski G., Rowinski J., Sulikowska-Rowinska A.

Folia Histochemica et Cytobiologica. Supplement

|

1996

|

tom 34

|

nr 1

7

Ultrastructural observations of nuclear bodies in the epithelial cells of human conjunctiva

63%

Skopinski P., Zmijewski M., Portacha L., Zamlynska A., Przyluski J.

Folia Histochemica et Cytobiologica

|

1993

|

tom 31

|

nr 4

Ograniczanie wyników

Arginine methylation analysis of the splicing-associated SR protein SFRS9-SRP30C

Nuclear bodies in paneth cells of the rat

Viruses as hijackers of PML nuclear bodies

Nuclear bodies in hepatocytes of the rat

PML shuttles between nuclear bodies and the cytoplasm

Ultrastructural components of the nuclei of undifferentiated crypt base cells in the human small intestine - stereological of volumes

Ultrastructural observations of nuclear bodies in the epithelial cells of human conjunctiva