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The aim of this study was to discern whether telomerase activity might serve as a marker for canine skin tumors. Telomerase activity was measured in 57 samples of tumor tissues from operated dogs. Telomerase activity in the samples was measured using the diagnostic kit Telo TAGGG Telomerase PCR ELISA plus of ROCHE - photometric enzyme immunoassay for quantitative determination of telomerase activity, utilizing the telomeric repeat amplification protocol (TRAP). The samples were also investigated histopathologically. The performed investigations permitted the following conclusions: telomerase activity is substantially higher in malignant lesions then in benign lesions, but there is no differences between telomerase activity in benign lesions and normal skin. Telomerase activity in normal skin near malignant tumors is substantially higher then in normal skin from healthy dogs and there is no correlation between telomerase activity in malignant tumors and in the normal skin in their proximity.
The aim of the study was to evaluate the TRAP assay method of measuring telomerase activity in canine skin neoplasms. Telomerase activity was measured in 57 samples of tumor tissues from operated dogs. The samples were also subjected to histopathology investigations. Telomerase activity was measured using the Telo TAGGG Telomerase PCR ELISA diagnostic kit as well as ROCHE . photometric enzyme immunoassay for quantitative determination of telomerase activity, utilizing the telomere repeat amplification protocol (TRAP). The repetition, sensitivity and specificity of telomerase activity measurements were determined. TRAP assay performed in dogs has high repetition rate within high activity, and lower within small activity. This method is 100% sensitive and 34% specific.
Inhibition of HIV virions release from infected cell by tetherin may find application in AIDS therapy. Is polyomavirus an etiologic agent in human Merkel cell skin carcinoma? Viral and bacterial interactions in living tissues are more complicated than in sterile cell lines used as models. Inhibition of severe and fatal outbreaks of feline calicivirus infections by antisense morpholino oligomer. Hitherto unknown important role of mast cells in immunity and pathogenesis. Participation of complement in some diseases and search for complement-targeted therapeutics.
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