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Spreading-independent growth of normal fibroblasts in three-dimensional cultures

100%

Czyz J., Rajwa B., Bzowska M., Dobrucki J., Korohoda W.

Folia Biologica

2004

tom 52

nr 1-2

Changes in GSH-antioxidant system induced by daunorubicin in human normal and diabetic fibroblasts

67%

Zatorska A., Maszewski J., Jozwiak Z.

Acta Biochimica Polonica

2003

tom 50

nr 3

We investigated the effect of daunorubicin on glutathione content and activity of GSH-related enzymes in cultured normal and diabetic human fibroblasts. Cells were incubated with 4 ^M daunorubicin (DNR) for 2 h followed by culture in drug-free medium for up to 72 h. Treatment of diabetic cells with the drug caused a time-dependent depletion of intracellular GSH and a decrease of the GSH to total glutathione ratio. GSH depletion was accompanied by apoptotic changes in morphology of the nucleus. Analysis of GSH-related enzymes showed a significant increase of the activities of Se-dependent and Se-independent peroxidases and glutathione ^-transferase. In contrast, glutathione reductase activity was reduced by 50%. Significant differences between normal and diabetic cells exposed to DNR were observed in the level of GST and Se-dependent glutathione peroxidase activities. These findings indicated that daunorubicin efficiently affects the GSH antioxidant defense system both in normal and diabetic fibroblasts leading to disturbances in glutathione content as well as in the activity of GSH-related enzymes.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Folia Biologica

1 Bzowska M.

1 Czyz J.

1 Dobrucki J.

1 Jozwiak Z.

1 Korohoda W.

1 Maszewski J.

1 Rajwa B.

1 Zatorska A.

1 2004

1 2003

Wyniki wyszukiwania

Spreading-independent growth of normal fibroblasts in three-dimensional cultures

Changes in GSH-antioxidant system induced by daunorubicin in human normal and diabetic fibroblasts