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We show that force plate measurements provide a noninvasive method to display the motion of the heart muscle and the subsequent propagation of the pulse wave along aorta and its branches. The aim of this paper is to present a new method to handle multivariate time series obtained by force plate measurements. The proposed technique is subsequently used to display marks of cardiac activity.
Estimates of water mass, lean body mass (LBM) and fat content based on total body electrical conductivity (TOBEC) measured with ACAN-2 were compared with direct measurements of carcass composition in two species of rodents: the laboratory mouse (n = 20) and the bank vole (n = 14). There was a strong linear relation between ACAN readings and lean mass or water mass. Body temperature did not affected the results, but the readings were dependent on the species and sex of the individuals. That could be due to differences in the body shape. The accuracy of predicting an average LBM or water mass was very high (1.5%), but the errors for predicting individual values were larger (8.5%). The predicted fat content values have identical confidence intervals as that for LBM. However, the relative error for the prediction of the amount of fat was higher, because the mass of fat was much smaller than LBM.
We used a non-invasive TOBEC method (Total Body Electric Conductivity) to estimate lean body mass and fat content in mouse-eared bats Myotis myotis (Borkhausen, 1797) hibernating in Poznań Forts (W Poland) and in a semi-natural cave-mine Miedzianka (SE Poland). In December, fat content averaged 5.5 g in females (body mass = 29.4 g) and 5.3 g in males (body mass = 28.4 g). At the end of hibernation (April), fat content averaged 2.2 g in females (body mass = 25.6 g) and 1.4 g in males (body mass = 23.7 g). Fat content did not differ between the localities either in December or in April, but the pattern of changes of fat content was different. We calculated the rate of energy expenditure in hibernating bats using two methods, based on independent samples (fat content in first-time captured individuals) and based on paired observations (changes of fat content in re-captured individuals), and discussed problems associated with the two approaches. Both methods show that the bats need about 4.9 g of fat (191 kJ) to sustain a 165-day hibernation. However, the rate of fat usage varied considerably between the sites and hibernation phase. Although the average amount of fat remaining in April would be sufficient to support at least six more weeks of hibernation, the level of reserves was close to zero in some individuals.
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