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Some basic experiments of nitrous oxide determination, utilizing a classic double beam spectrometer were performed. Known amounts of nitrous oxide were measured in atmospheric air, helium and exhaust gases. The experiments allowed determination of N20 in amounts of 80 and 4 ppmv, employing standard and long-path gas cells, respectively.
The actual denitrification to N 2 O and denitri- fication capacity to N 2 O after flooding of different soil samples stored for over 25 years in air-dry conditions and fresh, air dried samples were compared in our study. Zero N 2 O release was ob- served from the stored soils but the fresh soil samples had very low actual denitrification to N 2 O. NO 3 - addition significantly increased the amount of N 2 O (denitrification capacity to N 2 O) released after flooding, which depended on the length of storage and type of soils and was much higher in stored soils. Prolonged exposure of the soils to drought conditions caused a greater decrease in the Eh value compared with the fresh soil. The total cumulative release of N 2 O from the stored and fresh soils was correlated with the reduced NO 3 - and organic C content in soils enriched with NO 3 - . Some soils showed the capability of N 2 O consumption. CO 2 release depended on the length of storage and type of soils under flooding after pro- longed drought. On average, CO 2 release was higher from the stored rather than fresh soils. The organic C content in the stored soils was generally lower than in the fresh soils, probably due to the storage effect. The cumulative CO 2 release from the stored soils was well correlated with the organic C while no correlation was observed for the fresh soil samples.
The main mechanism responsible for N20 production in agroecosystems is denitrification of nitrates (V) under hypoxic conditions, which occur e.g., after intensive precipitation or at high ground water level. The calculations of potential denitrification were performed for surface horizons from 672 soil profiles representing 25 main soil units of Polish soils. A proposal of the assessment of potential denitrification rate on the basis of the nitrate content, humus horizon thickness, its bulk density and of previously investigated soil redox resistance in the form of indicators tm and tm is presented. The proposal is based on the assumption that denitrification is linear with time and is illustrated for the temperature of 20°C. It was shown, that the highest potential denitrification would occur in Rendzic Leptosols and in the soils developed from loess i.e. Phaeozem, Cambisol and Luvisol, while the lowest <5 kg ha-1d-1 N - in Luvisols developed from loose sands, loams and skeleton loams. Evaluation of spatial distribution of the potential denitrification rate at current nitrate content in mineral soils of Poland has been attempted in the form of a map.
Celem pracy było określenie wpływu poziomu azotanów w glebie na tempo denitryfikacji, mierzonej ubytkiem azotanów oraz ilością emitowanego podtlenku azotu. Do badań wybrano: glebę płową- Apglp.glp oraz dwie gleby brunatne - Bli i Bpgm-gc.sk, które wzbogacono dawkami azotanów do wartości odpowiadającej nawożeniu 100 i 200 kg N-NO3/ha. Emisja podtlenku azotu z gleby rozpoczęła się już w pierwszych trzech dniach, a jej wielkość maksymalna zależała nie tylko od dawki wprowadzonych azotanów (V), ale była przede wszystkim związana z cechami rodzajowymi gleby, które określają jej potencjalną zdolność do denitryfikacji i przy tym samym poziomie azotanów (V) była dwukrotnie większa w glebie brunatnej, w której zawartość węgla organicznego wynosiła 0,98% w porównaniu z glebą A o poziomie węgla organicznego równym 0,73%.
Nitrous oxide (N₂O) was one of the major atmospheric greenhouse gases. Its budget was poorly understood in alpine meadow, a dominant vegetation type on the Tibetan Plateau. To characterize a Kobresia humilis meadow on the plateau, N₂O emission rates were monitored from June 2003 to June 2006 in the study area located at 3280 m a.s.l. Nine plots with 1 m × 1 m each were divided into three treatments, i.e. intact herbaceous community (HCK), removal of aboveground plant biomass (CBK), and removal of both above and belowground plant biomass (BSK), to estimate contribution of plants, r hizosphere and bulk soil to the total N₂O emission. N₂O emission from plant aboveground biomass was calculated by flux difference between HCK and CBK, denoted as F (HCK-CBK), from rhizosphere by F (CBK-BSK), and from bulk soil was the flux in BSK treatment. Static chambers (height 50 cm, area 0.5 × 0.5 m²) were used for gas collection. N₂O emission rate was significantly correlated with soil temperature at 5 cm depth in both HCK and BSK (P <0.001). Both treatments demonstrated a seasonal peak rate in growing season and minimum rate in dormancy period. The mean emission rates in the three years were 39.7±2.9 and 30.6±2.5 μg m⁻² h⁻¹ in HCK and BSK, respectively, with the former significantly higher than the latter (P <0.05). In CBK, however, the emission rate did not show consistent correlation with soil temperature, especially in growing season. Its three-year mean emission rate was 36.2±3.3 μg m⁻² h⁻¹. In the K. humilis meadow, bulk soil contributed much more than plants and rhizosphere. The mean emission rate was 3.5±2.9, 5.7±3.8, and 30.6±2.5 μg m⁻² h⁻¹ (P <0.001) from plants, rhizosphere and bulk soil, and these accounted for 9, 14 and 77%, separately. Our results implied that N₂O emission rate decreased little with grazing as indicated by the difference between HCK and CBK in K. humilis meadow (P <0.05). N₂O emission from alpine meadow could not be ignored in addressing regional greenhouse gases budget on the Tibetan Plateau, considering the vast area and much higher radiative forcing of N₂O.
The application of large amounts of organic or mineral nitrogen (N) fertilizers to soil may cause high risk of nitrogen (N) losses to air and groundwater. A field experiment on regularly cut (5-6 times per year) grassland located on sandy soil was established to evaluate the effect of different fertilization regimes (control without fertilizer, calcium ammonium nitrate (CAN 100), CAN 50/50 in a split dose, ammonium sulphate (SACAM), cattle slurry in 3 doses (SLUR 20/20/20) of 20 tons of cattle slurry in 2 doses (SLUR 30/30) of 30 tons of manure with additional CAN to equal amounts of N added with mineral fertilizer only) on nitrogen losses via N20 production and emission and N03- leaching to deeper soil layers. For better evaluation of used practices (various N fertilization regimes) nitrogen balances for fields have been estimated. Highest fluxes of N20 were found on fields where slurry was applied. Application of slurry in three doses instead of two decreased emissions. Peak fluxes were found two weeks after application (145 μg m-2 h -1 N), and remained so for at least one month. A second fertilization caused another higher peak on the same fields (205 μg-m2-h-1 N). Mineral fertilization caused lower N20 emissions than slurry: Calcium ammonium nitrate used in one dose caused highest fluxes for mineral fertilizers with a peak also two weeks after application (70 μg m-2 h-1 N). On plots where CAN was divided into two rates, fluxes were lower and similar to plots where ammonium sulphate was applied. Generally, fluxes observed in this experiment were low. This could be attributed to a relatively low mineral N content in soil at the beginning of the growth period. Accumulated losses of N20 during the vegetation period until the first cut were highest for the SLUR 30/30 treatment (slurry 30 m3 at the first and 30 m3 at the third cut) and lowest for the CONTROL treatment.
Nitrous oxide (N2O) emission and absorption and carbon dioxide (CO2) emission from Eutric Cambisol were measured after treatment soils with NO3 - N in laboratory incubation experiments. Incubation experiments were designed to investigate the influence of variables such as oxidation- reduction conditions, pH, organic matter content and granulometric composition on denitrification capacity of investigated soils. Results showed that these soils were emitters (cumulative production N2O ranged from 11.4 to 66.5 mg N2O-N kg-1 of soil) as well as reducers (daily sink of N2O ranged from 1.3 to 66.5 mg N2O-N d-1 kg-1 of soil). The range of reduction of N20 under investigation conditions was from 10 to 100% depending on the kind of soil and time of incubation. Production and reduction of N20 were non- linearly correlated with redox potential (R2=0.906 and R2=0.966, respectively). The boundary value of redox potential for emission of nitrous oxide was 250 mV and for absorption of N2O was about 200 mV. Redox potential showed a negative correlation with pH value (R =0.685). Eh value decreased with decreasing of N03-N in the range from about 10 to 100 mg NO3-N kg-1 of soil. The highest daily reduction of nitrate was observed in the narrow range of Eh value (200 - 210 mV). The boundary nitrate concentration resulted in distinct drop of redox potential was about 100 mg NO3-N kg-1. Under investigated conditions the maximum emission of N20 was observed at pH range between 4.5-6, but maximum absorption of nitrous oxide occurred at pH about 5.5 to about 7. Differences in the dissimi- lative reduction of nitrate were observed in the investigated soils. Absorption of N20 occurred simultaneously with the reduction of nitrate and after depletion of NO3- during the course of the experiment. The range of reduccd nitrate fluctuated from 22 to 100% depending on the kind of soil and time of incubation. Denitrification rate and sink of nitrous oxide showed high cor- relation with mineralization of organic matter (R =0.906 and R =0.913, respectively). Daily emission of CO2 ranged from 1.3 to 23.2 mg CO2-C kg-1 d-1 and was correlated with redox potential (R2=0.871), organic matter content (R2=0.314), and pH (R2=0.804). Differences in release of C02 were noticed in the investigated soils. The lowest cumulative amount of carbon dioxide was found in soils developed from sand (mean 75 mg C02-C kg-1). Higher emission was observed in soils developed from loam (mean 130 mg CO2-C kg-1) and the highest amount of carbon dioxide was obtained in soils developed from silt (mean 210 mg C02-C kg-1). Relationship between carbon (as CO2) and nitrogen (as N2O) flux reflected by the C/N ratio in the headspace air and relationship between carbon (as CO2 flux) and nitrogen (as NO3- reduced) were calculated. These two ratios describe the character of biochemical processes in the soils particularly their denitrification capacity as well as their ability to sink of nitrous oxide and nitrate reduction.
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