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1

Plant ureases: roles and regulation

100%
Sirko A., Brodzik R.
Acta Biochimica Polonica
|
2000
|
tom 47
|
nr 4
Both urea and urease were subjects of early scientific investigations. Urea was the first organic molecule to be synthesized and jack bean urease was the first enzyme ever to be crystallized. About 50 years later it was shown to be the first nickel metalloenzyme. Since then, nickel-dependent ureases have been isolated from many bacteria, fungi and higher plants. They have similar structures and mechanisms of catalysis. A urease apoenzyme needs to be activated. This process requires participation of several accessory proteins that incorporate nickel into the urease forming catalytic site. In this review, ureases from various organisms are briefly described and the similarities of their structures discussed. Moreover, the significance of urea recycling in plants is explained and recent literature data about the function and activation of plant ureases are presented.
2
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Plant peroxidases and nitrogen metabolism: interactions with nitrates and nitrites

86%
Minibayeva F., Galeeva E., Gurjanov O., Viktorova L.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2013
|
tom 94
|
nr 2
3
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Activities of nitrogen metabolism-related enzymes in cucumber leaves infected with Pseudomonas syringae pv.lachrymans

72%
Gajewska E., Kuzniak E., Naliwajski M., Sapinska S., Wielanek M., Sklodowska M.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2013
|
tom 94
|
nr 3
4

Renal urea excretion in single- and twin-pregnant goats

72%
Michalek K., Ozgo M., Skrzypczak W. F.
Electronic Journal of Polish Agricultural Universities. Series Veterinary Medicine
|
2009
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tom 12
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nr 1
5

Professor Jan Kowalczyk on the 50th anniversary of his professional career and 70th birthday

72%
Zebrowska T.
Journal of Animal and Feed Sciences. Supplement
|
2001
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tom 10
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nr 1
6

Asparagine and glutamine in the leaves of high and low protein maize

72%
Dembinski E., Bany S., Raczynska-Bojanowska K.
Acta Physiologiae Plantarum
|
1995
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tom 17
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nr 4
7

Comparative study of selected blood biochemical components in milk or milk-replacer fed calves during the second week of life

58%
Lepczynski A., Herosimczyk A., Dratwa-Chalupnik A., Ozgo M., Michalek K., Malinowski E., Skrzypczak W. F.
Folia Biologica
|
2011
|
tom 59
|
nr 3-4
8
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The effect of exogenous proteases on selected hematological and serum biochemical parameters in boars

58%
Zielonka L., Przewoski W., Gajecka M., Jakimiuk E., Gajecki M.
Polish Journal of Veterinary Sciences
|
2010
|
tom 13
|
nr 1
151-156
The increasing interest in enzymatic growth promoters prompted this investigation of the effect of an enzymatic stimulating complex on selected hematological and serum biochemical parameters in boars. The enzymatic complex [five proteases (proteinases-endopeptidases) and two peptidases (exopeptidases) obtained by fermentation from Streptomyces fradiae] was added to diets for three months, at initial doses of 90 (group El) and 120 (group E2) g/ton feed for the first seven days followed by 40 and 60 g/ton, respectively, in the subsequent weeks. The evaluation was based on selected hematological (RBC, WBC, HCT (Ht), HGB (Hb), MCV, MCH, and MCHC) and biochemical (AST, ALT, AP, Ca, Mg, urea, cholesterol, creatinine, inorganic phosphorus, and oxyglucose) parameters determined before and after the experimental period. The reported values were within physiological norms. Statistical differences were found between experimental groups with respect to WBC, HCT (Ht), MCV, MCH, AP, Ca, Mg, creatinine, and inorganic phosphorus. The study results indicate that the complex of proteolytic enzymes administered to boars caused no negative changes in their metabolic profiles. The statistically different hematological and serum biochemical parameters, while within normal limits, point to an early stage of microcytic anemia and heightened agitation that could be the result of intensified nitrogen metabolism.
9

Improving plant sulfur nutrition: from sulfate transporters to the transcriptome

58%
Hawkesford M. J.
Biological Letters
|
2005
|
tom 42
|
nr 2 Spec.Vol.
10

Effects of dietary protein and carbohydrate levels on growth performance, feed utilization efficiency and nitrogen metabolism in rohu, Labeo rohita [Hamilton], fingerlings

58%
Dalal S., Bhattacharya S., Ray A. K.
Acta Ichthyologica et Piscatoria
|
2001
|
tom 31
|
nr 2
A 60-day feeding experiment was conducted in the laboratory to evaluate the interactive effects of dietary protein and carbohydrate levels on growth, feed utilization efficiency, and nitrogen metabolism in rohu, Labeo rohita fingerlings (mean weight 4.06 ±0.08 g). Nine purified diets prepared with 25, 35, and 40 % protein level each having 15, 25, and 35 % carbohydrate were fed to nine different feeding groups at the rate of 3% of body weight in triplicate treatments. Better performance of fish in terms of percent weight gain, SGR, FCR, and PER was observed with increasing percentage of carbohydrate at a given protein level. Protease and α-amylase activities increased with increase in dietary protein and carbohydrate levels, respectively. Glutamate oxaloacetate transaminase (GOT) activities varied significantly in some groups. No significant difference among different groups regarding glutamate pyruvate transaminase (GPT) activity was observed. Ammonia excretion was found to increase with increased consumption of dietary protein and carbohydrate and was highest in the groups of fish fed 40% protein diet suggesting active nitrogen metabolism in these.
11

Effects of UV-B irradiation on growth, survival, pigmentation and nitrogen metabolism enzymes in cyanobacteria

58%
Sinha R. P., Kumar H. D., Kumar A., Hader D. P.
Acta Protozoologica
|
1995
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tom 34
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nr 3
12

The activity changes of some enzymes of respiratory pathways and nitrogen metabolism under salt and osmotic stress in lupin seedlings

58%
Mossor-Pietraszewska T., Kocur M., Mazur H.
Biological Bulletin of Poznań. Supplement
|
1997
|
tom 34
13
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The effect of plant biostimulation with ‘Pentakeep V’ and nitrogen fertilization on yield, nitrogen metabolism and quality of spinach

51%
Smolen S., Sady W., Wierzbinska J.
Acta Scientiarum Polonorum. Hortorum Cultus
|
2010
|
tom 09
|
nr 1
Foliar application of fertilizers containing biostimulators can influence yield and the uptake and accumulation of NO3- by plants. The aim of the research was to determine the influence of foliar nutrition with ‘Pentakeep V’ and diverse doses of nitrogen fertilization on yield, plant nitrogen metabolism and nutritional quality of spinach. The experiment design included two sub-blocks with and without foliar nutrition. Plants with foliar nutrition were sprayed twice with 0.02% w/v ‘Pentakeep V’ fertilizer (3000 dm3 water per 1 ha). In each sub-block soil nitrogen fertilization (in the form of ammonium nitrate) was applied in following combinations: 1 – control (without N), 2 – 50% N dose prior to seed sowing (25 mg N∙dm-3 of soil), 3 – 100% N dose prior to seed sowing (50 mg N∙dm-3 of soil). The interaction between foliar nutrition and nitrogen fertilization had a significant effect on the content of: nitrates(V), soluble oxalates and ascorbic acid in spinach leaves. The effect of tested factors on the content of nitrates(V) and ascorbic acid was additionally modified by the weather conditions in both years of research. Foliar application of ‘Pentakeep V’ resulted in decreased concentration of soluble oxalates in control plants (without N) as well as higher amount of these compounds in plants fertilized with 100% of N dose. No significant effect of tested interaction was observed in the case of yield and the content of: dry matter, nitrates(III), ammonium ions, free amino acids, N-total, soluble sugars and phenolic compounds in spinach leaves.
14
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The effect of diverse iodine fertilization on nitrate accumulation and content of selected compounds in radish plants (Raphanus sativus L.)

44%
Strzetelski P., Smolen S., Rozek S., Sady W.
Acta Scientiarum Polonorum. Hortorum Cultus
|
2010
|
tom 09
|
nr 2
Plant fertilization with iodine may be an alternative source of this element in human diet. Iodine influence on nitrogen metabolism in plants has not yet been thoroughly described. Thus, there is an urgent need to determine the effect of iodine application on nitrate(V) accumulation in plants. The aim of the study was to determine the influence of soil and foliar application of iodine forms (I-, IO3-) on nitrate accumulation and concentration of selected compounds in radish plants. The following treatments were applied in the experiment: 1 – control (without application of iodine), 2 – foliar application in KI form, 3 – foliar application in KIO3 form, 4 – soil fertilization in KI form, 5 – soil fertilization in KIO3 form, 6 – soil fertilization in KI form + foliar application in KI form, 7 – soil fertilization in KIO3 form + foliar application in KIO3 form. Soil fertilization with iodine was carried out before radish sowing to the level of 15 mg I·dm-3 soil. Foliar application of this element was performed twice using iodine solution in a concentration per pure element of 0.2%, in dose of 0.4 dm3· m-2. In all tested combinations with iodine treatment an increase of ammonium ion content in radish roots was observed in comparison to the control. Both, foliar nutrition with KI as well as nitrogen fertilization with KIO3 (combination 2 and 6, respectively) resulted in a significant increase of free amino acids concentration in radish roots. No significant influence of tested factors was noted for the root and leaf content of: dry mass, nitrates(V), nitrates(III) as well as root level of total soluble sugars and leaf concentration of photosynthetic pigments and ammonium ions in radish.
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