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The dependence of the content of various forms of nitrogen and absorption of N by cocksfoot grass on meteorological conditions was established in a field experiment. Correlations between the content of total nitrogen, protein nitrogen, N-NH4, and N-NO3 in sward and roots of cocksfoot grass and some meteorological elements, i.e. maximum temperature, minimum temperature, average daily temperature measured at 5 and 200 cm, air relative humidity, cloud cover, sum of precipitation, sum of evaporation and soil temperature measured at a depth of 2, 5, 10, 20 cm were analyzed. Correlation coefficients between the forms of nitrogen in the plant and the above meteorological elements were calculated, as well as multiple regression equations, multiple correlation coefficients and determination coefficients. No significant relationship between the forms of N (Ntotal, Nprotein, N-NH4, N-NO3) in sward and roots of cocksfoot grass and the course of meteorological conditions was clearly stated. The relationship between the content of N-NH4 in sward and Ntotal and N-NO3 in roots, and certain meteorological elements is relatively small and can be characterized by the value of determination coefficients, i.e. 0.166, 0.106 and 0.151, respectively. According to the statistical analysis, the absorption of nitrogen by cocksfoot grass depends to a relatively small though significant extent (R2=0.249) on certain meteorological elements. However, further research is still recommended.
The purpose of the investigation was the assessment of the effect of fertilization with different form and dose of nitrogen as well as harvest term on yield, chlorophyll, carotenoids and polyphenols content, and antioxidant activity of stinging nettle (Urtica dioica L.) leaves. The lowest total yield obtained from three-year-lasting plantation resulted from preplant fertilization with lime saltpeter. The highest yield of raw material was provided by nitrogen fertilization in the dose of 150 kg N/ha. Total content of polyphenols in nettle leaves collected in May amounted, counted over gallic acid, 7.13–9.46 mg∙g-1 d.m. and was by 40–50% higher than that assayed in July. Chlorophylls (7.72–9.63 mg∙g-1 d.m.) and carotenoids (0.74–0.96 mg∙g-1 d.m.) content was lower in May by 20–40% than the one in nettle leaves collected in July. In the course of effect of nitrogen preplant fertilization plants accumulated higher amounts of polyphenols when treated with N saltpeter form. Increased nitrogen dose from 50 to 150 kg N∙ha-1 contributed to the increase in chlorophyll and carotenoids content in nettle leaves and, to a lower degree, to differentiating polyphenols level. Harvest term did affect antioxidant activity of raw material more significantly than nitrogen form and dose used in the experiment. Raw material collected in May characterized higher antioxidant activity measured according to FRAP and ABTS.
Eggplant fruits are known for being low in calories but rich in minerals, which is good for human health. They are rich in potassium, whose content ranges from 200 to 600 mg K⋅100 g-1 of fresh mass, depending on a cultivar. Eggplant fruits are also a source of magnesium, calcium and iron. Research on the agro-techniques of eggplant culture in a plastic tunnel has implicated that, on account of a very intensive growth of the plant, both plant pruning and training have a decisive influence on the final amount of fresh mass. Since we lack information concerning the fertilization recommendation for growing eggplants under a plastic tunnel, a study has been undertaken to specify such nutritional needs of this vegetable. The aim of this work has been to determine the influence of nitrogen forms and plant training methods on the content of nitrogen, phosphorus, potassium, calcium and magnesium in eggplant fruits. The experiment on cv. Epic F1 eggplant was carried out in years 2004-2005, with eggplants growing in an unheated plastic tunnel. The eggplants were cultivated in cylinder plastic wraps of 10 dm3 volume, in peat. The experiment was carried out in two stages, in a completely random design, with each stage examining different factors. The following factors were examined: I – nitrogen forms: NH4 + (ammonium sulphate – (NH4)2SO4 (20,5% N); NO3 - (calcium nitrate – Ca(NO3)2 (15,5% N); NH2 (urea – CO(NH2)2 (46% N), II – plant training method: natural form of the plant, 3 shoots. Nitrogen was used in the amount of 10 g N⋅plant-1. Samples of fruits used for further laboratory tests were collected in the 2nd decade of August, in the middle of fructification. The fruits were harvested at the marketable stage. N-total, P, K, Ca, Mg were determined in the fruits. The results were elaborated statistically using analysis of variance. Generally, considerably higher content of nitrogen was determined in eggplant fruits fertilised with the N ammonium form; also the content of potassium and magnesium was much higher in comparison to the other nitrogen forms examined. Moreover, significant influence of the plant pruning method on the content of the elements was found, independently of the applied nitrogen fertilization.
The influence of different nitrogen forms: (N2), [N2+(NH4++NO3-)], (NH4+), (NO3-), (NH4++NO3-) and (-NH2) on changes of albumin, globulin, prolamin-glutelin, non-fractioned nitrogen and non-protein nitrogen fractions of the protein seed of alkaloid-low content Lupinus albus L. cv. Butan has been studied. The experiments were performed in a greenhouse on perlite using in all cases the constant P, K, Mg and micronutrients (B, Zn, Mn, Cu, Mo, Fe) fertilization. The control was the treatment without any nitrogen support (Nd). It was clearly shown that nitrogen form has significant effect not only on the seed yield and seed protein content, but also on the composition of protein fractions and on the biological value of lupin protein. The main protein fraction of the seeds from all treatments were albumins (16.73-26.10 mgN/g). Among all the treatments, the highest level of globulin was observed for the seeds of plant growing with the symbiotic nitrogen form (15.26 mgN/g), while the lowest one for the control (Nd) (6.86 mgN/g). Symbiotic nitrogen (N2) treatment clearly increased the glutelin-prolamin fraction while the addition of mineral nitrogen (NH4++NO3-) decreased this fraction from 8.40 to 4.48 mgN/g. The lowest level of the glutelin-prolamin fraction was in the absence of any nitrogen (Nd). Non-protein fraction (Nnp) was highest in the case of plants treated with (-NH2) (9.92 mgN/g), and the lowest in the absence of nitrogen (Nd) (4.90 mgN/g). The level of non-fractioned nitrogen (Nr), with exception of [N2+(NH4++NO3-)] and -NH2 treatments, was closest to the start material. The protein fractions (albumins, globulins and glutelins and prolamins) were also electrophoretically characterized. These analysis confirmed the changes in protein composition of particular fractions under the effect of various nitrogen forms used as a fertilizer.
The study assessed the effect of various forms of sulphur (Na2S2O3, elemental S and Na2SO4) and nitrogen (UAN-30, NH4NO3) on calcium and magnesium content and uptake in spring wheat and cocksfoot. A two-year pot experiment was conducted on soil material of clayey silt granulometric composition. Before the experiment, the soil was characterized by slight acidity and low content of assimilable forms of phosphorus, potassium, magnesium and sulphur. The results show that the experimental factors caused noticeable variation in the content and uptake of calcium and magnesium in spring wheat and cocksfoot. Among the sulphur fertilizers, the greatest increase in calcium and magnesium content and uptake was produced by application of sodium sulphate. The increase in calcium and magnesium uptake was more marked in the case of plants fertilized with nitrogen in the form of ammonium nitrate. The effect of nitrogen fertilization on the analyzed parameters also depended on the species of plant. Wheat grown in the series with ammonium nitrate was characterized by higher Ca and Mg content and uptake than plants fertilized with nitrogen in the form of liquid UAN 30. Calcium uptake by wheat fertilized with NH4NO3 was on average 15% higher for grain and 9% higher for straw compared to wheat fertilized with UAN 30. As for magnesium, the increase was 24.5% and 18%, respectively. Concerning cocksfoot, fertilization with UAN 30 had a greater affect on content and uptake of the analyzed nutrients than ammonium nitrate.
In July 2000 the diurnal cycle of analyses was conducted in order to determine variation in the concentrations of nitrogen and calcium compounds as well as dissolved gases in surface microlayers of a shallow coastal barrier lake located at the Baltic Sea coast. Water for analyses was collected in a diurnal cycle for the period of 24 h from lake Dołgie Wielkie. The analyses were conducted on the surface microlayer with a thickness of approx. 100 μm, a layer with a thickness of approx. 250 6m and a layer of subsurface water from a depth of 15 cm. Based on the analyzed data, information was collected on the migration of nitrogen compounds between the analyzed layers. Changes were observed in nitrogen forms reduced to oxidized forms and vice versa.
The objective of the study was the estimation of quality of organic matter of the humus horizon of soil fertilised every year, for ten years, with spent mushroom (Agaricus L.) substrate, as compared to a soil without such amendment. Fractionation of organic matter was performed, as well as quality analyses of humic acids and fractionation of organic nitrogen complexes. In the humus horizon of the soil fertilised with spent mushroom substrate lower acidity was noted, and higher levels of C, N, P and Ca. In the soil amended with the substrate greater accumulation of soil humus was noted (by nearly 50%), and especially of the fraction of humic acids, which had a positive impact on increasing the value of the ratio of humic to fulvic acids. The fertilisation with the substrate only slightly modified the quality parameters of humic acids. In the soil fertilised with the substrate a higher level of carbon and nitrogen was observed – related with the soluble forms of organic matter, as well as greater accumulation of nitrogen, mainly in a stable non-hydrolysing form.
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