Wyniki wyszukiwania

1

NADPH-diaphorase in porcine uterus after infusions of Escherichia coli endotoxin

100%

Jana B., Andronowska A., Kucharski J., Skwarski R.

Folia Histochemica et Cytobiologica

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1999

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tom 37

|

nr 2

2

An immunohistochemical study on the presence of nitric oxide synthase isoforms (nNOS, iNOS, eNOS) in the spinal cord and nodose ganglion of rats receiving ionising gamma radiation to their liver

75%

Yilmaz O., Soyguder Z., Keles O. F., Yaman T., Yener Z., Uyar A., Cakir T.

Journal of Veterinary Research

|

2020

|

tom 64

|

nr 3

3

The transcriptional cascade associated with creatine kinase down-regulation and mitochondrial biogenesis in mice sarcoma

75%

Bera S., Ray M.

Cellular and Molecular Biology Letters

|

2009

|

tom 14

|

nr 3

481-496

The tissue-specific expressions of creatine kinase (CK) isoforms are regulated by the coordinated action of various transcription factors. The myogenic differentiation factor D (MyoD) family of proteins and the myocyte-specific enhancer binding factor 2 family of transcription factors are important in regulating the muscle-specific expression of cytosolic muscle-type CK (MCK) and mitochondrial CKs. As reported in some related studies, TNF-α mediated degradation of MyoD and myogenin mRNA may lead to severe muscle wasting and cachexia, which is characterized by a low transcript level of MCK and myosin heavy chain proteins. In our previous study, we reported on a complete loss of total CK activity and expression when sarcoma was induced in mouse skeletal muscle (Patra et al. FEBS J. 275 (2008) 3236–3247). This study aimed at investigating the transcriptional cascade of CK down-regulation in carcinogen-induced sarcoma in mouse muscle. Both CK deficiency and enhanced nitric oxide synthase (NOS) were known to augment mitochondrial biogenesis, so we also explored the activation of the transcriptional cascade of mitochondrial biogenesis in this cancer. We observed the activation of the TNF-α-mediated nitric oxide production pathway with NFκB activation and concomitant degradation of MyoD and myogenin mRNA. Exploration of mitochondrial biogenesis revealed high cytochrome c oxidase activity and mitochondrial DNA content in sarcoma. The PGC-related co-activator seems to have a major role in regulating mitochondrial biogenesis by upregulating nuclear respiratory factors and mitochondrial transcription factor A. From the above findings, it can be concluded that severe muscle degeneration leads to CK down-regulation in sarcoma, and that the stimulation of mitochondrial biogenesis indicated a scenario representing both CK deficiency and NOS overexpression on the one hand, and altered bioenergetic profiling on the other.

4

An ultrastructural demonstration of NADPH-diaphorase-nitric oxide synthase activity in the rat striatal astroglia

75%

Calka J., Wolf G.

Folia Morphologica

|

2003

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tom 62

|

nr 3

251-253

In the present study we have used electron microscopical NADPH-diaphorase (NADPH-d) histochemistry as a visualization procedure for nitric oxide synthase (NOS) to examine patterns of activity in the subcellular distribution of NADPH-d in the rat striatal astroglia. Electron microscopical examination revealed deposition of the NADPH-d reaction product in a nuclear envelope, fragments of endoplasmic reticulum and mitochondria. Predominantly mitochondria of astrocytic “end feet” were labelled. Our ultrastructural observations promote the possibility that astroglial NADPH-d/NOS is involved in adaptation of the local blood flow in the striatal microenvironment.

5

Effect of leptin on thyroid-stimulating hormone secretion and nitric oxide release from pituitary cells of ewe lambs in vitro

75%

Radwanska P., Kosior-Korzecka U.

Journal of Physiology and Pharmacology

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2014

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tom 65

|

nr 1

6

The expression of inducible nitric oxide synthase [iNOS] in the testis and epididymis of rats with a dihydrotestosterone [DHT] deficiency

75%

Kolasa A., Marchlewicz M., Kurzawa R., Glabowski W., Trybek G., Wenda-Rozewicka L., Wiszniewska B.

Cellular and Molecular Biology Letters

|

2009

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tom 14

|

nr 3

511-527

In our previous studies, we showed that a finasteride-induced DHT deficiency may cause changes in the morphology of the seminiferous epithelium without any morphological alteration of the epididymis. In this study, we demonstrated the constitutive immunoexpression of inducible nitric oxide synthase (iNOS) in the testis and epididymis of Wistar rats treated with finasteride for 28 days (the duration of two cycles of the seminiferous epithelium) and 56 days (the duration of one spermatogenesis). We noted that a 56-day finasteride treatment mainly caused a decrease in the level of circulating DHT, as well as a statistically insignificant decrease in the level of T. The hormone deficiency also led to a change in the iNOS immnoexpression in the testis and epididymis of the finasteride-treated rats. In vitro, DHT did not modify NO production by the epithelial cells of the caput epididymis even when stimulated with LPS and IFNγ, but it did give rise to an increase in NO production by the epithelial cells of the cauda epididymis without the stimulation. DHT did not have a statistically significant influence on estradiol production by cultured, LPS- and IFNγ-stimulated epithelial cells from the caput and cauda epididymis. In conclusion, our data clearly indicates that a finasterideinduced DHT deficiency intensifies the constitutive expression of iNOS in most rat testicular and epididymal cells, so it can be expected that the expression of inducible nitric oxide synthase (iNOS) could be regulated by DHT. On the other hand, the profile of the circulating DHT and T levels strongly suggests that the regulation of constitutive iNOS expression is complex and needs more detailed study.

7

The effect of fiberoptic bronchoscopy on exhaled nitric oxide

75%

Krenke R., Przybylowski T., Hildebrand K., Fangrat A., Gorska K., Barnas M., Chazan R.

Journal of Physiology and Pharmacology. Supplement

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2006

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tom 57

|

nr 4

183-190

Nitric oxide has been extensively studied as a noninvasive marker of airway inflammation. Assuming that bronchoscopy can produce not only systemic but also local inflammatory response, we hypothesized that bronchofiberoscopy can be responsible for an increase in nitric oxide synthesis with resulting increase in fractional concentration of exhaled nitric oxide (FENO). Fifty five subjects (F/M-23/32; mean age 53.9 ±17.3 yr) undergoing diagnostic bronchoscopy participated in the study. The indications for bronchoscopy were as follows: interstitial lung diseases (n=13; 23.6%), lung cancer (n=11; 20.0%), hemoptysis (n=10; 18.2%), differential diagnosis of asthma/dyspnea (n=9; 16.4%), pulmonary infections (n=7; 12.7%), and others (n=5; 9.1%). During bronchoscopy bronchial washing (n=18), bronchoalveolar lavage (BAL) (n=26), and bronchial biopsies (n=24) were performed. FENO was analyzed on-line with chemiluminescence analyzer (NIOX, Aerocrine, Sweden) according to the ATS guidelines, before and at 1, 2, 3 and 24 h after bronchoscopy. The mean FENO before bronchoscopy was 21.0 ±3.31(SE) ppb, it decreased to 14.8 ±2.10 ppb 1 h after bronchoscopy, reached a nadir at 2 h (14.4 ±2.28 ppb; P<0.05), and was not different from baseline 24 h after bronchoscopy (22.8 ±2.90 ppb). There were no differences in the FENO profile in BAL patients compared with those in whom only the bronchial washing was performed. We conclude that bronchoscopy leads to a decrease in FENO. The underlying mechanisms are at present unclear.

8

Distribution and morphology of the NOS-immunoreactive neurons in the thoracolumbar and sacral spinal cord of the pig

75%

Calka J., Zalecki M., Wasowicz K., Lakomy M.

Medycyna Weterynaryjna

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2006

|

tom 62

|

nr 10

1134-1138

The study investigated the distribution and morphology of nitric oxide synthesis-immunoreactive neuronal cell bodies and processes in the thoracolumbar and sacral spinal cord of sexually immature gilts. Investigations revealed the following: NOS-immunoreactive fibers and singular cell bodies in regions of the dorsal horn including superficial laminae I and II and deeper laminae III and IV; prominent NOS-immunolabeled perikarya in intermediolateral nucleus of the thoracolumbar spinal segments; NOS-positive perikarya in lamina X along the thoracic, lumbar and sacral divisions of the cord; NOS-positive perikarya and fibers in the area near to the location of the intermediomedial and intermediolateral nuclei in the sacral segments of the cord. The obtained morphological results indicate that the general distribution pattern of NOS-positive neurons in the spinal cord of pigs resembles that of other species. The concentration of NO-ergic neurons in the autonomic nuclei, dorsal horn laminae I, II, III, IV and lamina X suggests a prominent role of NO-ergic neurons in visceral and sensory functions.

9

Developmental changes in nitric oxide synthase protein expression in the rat striatum and cerebral cortex

75%

Labuda C., Litwinowicz B., Kowianski P., Spodnik J. H., Luczynska A., Morys J.

Folia Morphologica

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2003

|

tom 62

|

nr 3

171-174

We examined the expression of brain nitric oxide synthase (bNOS) in two developing rat brain structures, the striatum and the cerebral cortex. For this purpose, we quantified the relative protein concentration level using the Western blotting method and densitometric scanning. 32 Wistar rats, divided according to survival period (P0-P120-postnatal days) were used in this study. Our results demonstrate that bNOS expression rises in these structures during the first week of postnatal life, reaching a maximum in the striatum on the 10th day and in the cerebral cortex on the 7th day of postnatal life. After the period of increase the expression declines and after the 14th day a stabilisation of bone protein concentration is observed, both in the striatum and the cerebral cortex. These changes in bone protein expression might be related to the important role of nitric oxide in the developing rat brain, especially in synaptogenesis, apoptosis and neurotransmission.

10

The role of nitric oxide synthase, superoxide dismutase and heme oxygenase-1 in the synthesis of vascular endothelial growth factor

75%

Dulak J., Grzenkowicz J., Cisowski J., Podhajska A., Jozkowicz A.

Cellular and Molecular Biology Letters

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2002

|

tom 07

|

nr Suppl.

11

The relationships between neurons containing dopamine and nitric oxide synthase in the ventral tegmental area

75%

Klejbor I., Domaradzka-Pytel B., Ludkiewicz B., Wojcik S., Morys J.

Folia Histochemica et Cytobiologica

|

2004

|

tom 42

|

nr 2

12

Endotoxaemia in rats: role of leucocyte sequestration in rapid pulmonary nitric oxide synthase-2 expression

75%

Gebska A., Olszanecki R., Korbut R.

Journal of Physiology and Pharmacology

|

2005

|

tom 56

|

nr 2

Nitric oxide (NO), depending on the amount, time and source of generation may exert both, protective and deleterious actions during endotoxic acute lung injury (ALI). Evaluation of the expression and localization of NOS isoforms in the lung of lipopolysaccharide (LPS) - treated rats may contribute to understanding the role of NO in pathogenesis of ALI. Tissue samples (lung, heart, liver, kidney and spleen) as well as peripheral blood polymorphonuclear cells (PMNs) were collected from control male Wistar rats and LPS - treated animals, 15, 30, 60, 120 and 180 min after LPS injection (2 mg kg-1 min-1 for 10 minutes, i.v.). Levels of NOS-2 and NOS-3 mRNA and protein in tissues and PMNs were estimated by RT-PCR, Northern blotting and Western blotting. Additionally, myeloperoxidase (MPO) activity in tissue samples was assayed. NOS-3 mRNA as well as protein were detected in lungs of control animals; pulmonary NOS-3 expression was not influenced by LPS. The induction of NOS-2 mRNA in rat lungs and in PMNs isolated from peripheral blood was observed 15 minutes after LPS challenge. In contrast, increase of NOS-2 mRNA in the heart, kidneys, liver and spleen was observed 2-3 hours after LPS injection. In all tissues rise in NOS-2 mRNA was followed after 1-2 hours by increase of NOS-2 protein. Importantly, progressive leukocyte sequestration in the lung parenchyma that started as early as 15 min after LPS injection was revealed only in the lungs; in other organs no significant changes in MPO activity were detected up to 180 min after LPS injection. In conclusion, infusion of LPS caused much more rapid expression of NOS-2 in lungs as compared to the heart, kidneys, liver and spleen. Early induction of NOS-2 may depend on the LPS-stimulated rapid neutrophil sequestration within lung vasculature and fast induction of NOS-2 in sequestrated neutrophils.

13

Effects of nitric oxide synthase inhibitor on exercise-mediated pro- and anti-oxidative balance in rat blood plasma

75%

Frankiewicz-Jozko A., Grudzinski I. P.

Roczniki Państwowego Zakładu Higieny

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2005

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tom 56

|

nr 4

Rats were subjected to different running trainings on a treadmill for 3 weeks, including continuous endurance and intermittent exercises at speed intensity of 10,22 and 30 m/min (°15), respectively. On the last day of the training period, the animals were dosed with Nw-nitro-L-arginine methyl ester, L-NAME (30 mg/kg b.w.), and they were further subjected to exhaustive running exercise (22 m/min; °15). Studies showed that inhibition of nitric oxide synthase (NOS) with L-NAME mitigated pro- and anti-oxidative (TB/TA) balance in the blood plasma of rats subjected to exhaustive exercise. Intermittent training before the exhaustive exercise enhanced L-NAME-induced effects on TB/TA levels in rats, but it was not observed in continuous endurance trainings

14

Correlation between NADPH-diaphorase and iNOS in bank vole Leydig cells in vitro and in testicular sections with the use of histochemistry and immunocytochemistry

75%

Koziel E., Kotula M., Andronowska A., Pierscinski A., Bilinska B.

Folia Histochemica et Cytobiologica

|

2000

|

tom 38

|

nr 2

15

Distribution of NADPH-diaphorase and nitric oxide synthase in porcine ovaries after intraovarian infusions of Escherichia coli endotoxin

75%

Jana B., Andronowska A., Kucharski J.

Polish Journal of Veterinary Sciences

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2000

|

tom 03

|

nr 3

The aim of this study was to determine whether pathological changes in Escherichia coli endotoxin (LPS, lipopolysaccharide)-infused ovary are associated with an increase in NO production, and whether inflammatory mediators produced by LPS-infused ovary can affect the contralateral one to stimulate NO synthesis. Therefore, the activity of NADPH-diaphorase (NADPH-d) and distribution of the inducible isoform of nitric oxide synthase (iNOS) used as indicators of the intensity of the inflammatory process were studied in porcine ovaries after unilateral infusions of LPS into the hilus of one ovary. Fourteen sexually matured gilts with controlled estrous cycle were used. The animals were randomly divided into two groups: I (control; n=7), and II (treated; n=7). In the group I, 1ml of saline was infused into the hilus of each ovary, on the 15th to the 19th day of the estrous cycle, twice a day (at 06:00 and 18:00). The gilts of the II group received 2 mg of LPS (serotyp 055:B5) in 1 ml of saline infused into the hilus of one ovary on the same days of the estrous cycle. At the same time, 1 ml of saline was infused into the contralateral ovary. The ovaries were collected on the 7th day of the next estrous cycle during a median laparotomy. Cryostat sections of the paraformaldehyde fixed ovarian tissues were stained histochemically to study the activity of NADPH-d and immunohistochemi- cally to investigate the distribution of iNOS. In the control group, the activity of NADPH-d was similar either in the corpora lutea, thecal and granulosa cells of follicles or endothelial cells of blood vessels in both ovaries. In the LPS-infused ovary, the activity of this enzyme was higher (P < 0.05 - 0.01) in thecal and granulosa cells of follicles and in endothelium of the blood vessels as compared to that found in the corresponding structures of the contralateral ovary. The highest activity of NADPH-d was observed in luteinizing thecal and granulosa cells of cysts which occured in both ovaries only after LPS infusions. In all the structures of both ovaries, the intensity of NADPH-d reaction was higher (P < 0.05 - 0.001) in LPS-treated gilts than that found in the control group. Light microscopic observations revealed the strongest immunostaining for iNOS in the structures of LPS-infused ovary. In the contralateral ovary, iNOS im- munoreactivity was weaker but still stronger than that found in the control group. This study revealed that in gilts infusions of LPS into the hilus of one ovary enhanced the activity of NADPH-d and iNOS in both the ovaries. However, the activity of these enzymes depended on the location of LPS infusions. The data obtained indicate also that locally synthesised NO can mediate an inflammatory effect of LPS in the ovaries.

16

Isoprenoid depletion by statins antagonizes cytokine-induced down-regulation of endothelial nitric oxide expression and increases NO synthase activity in human umbilical vein endothelial cells

75%

Jantzen F., Konemann S., Wolff B., Barth S., Staudt A., Kroemer H. K., Dahm J. B., Felix S. B., Landsberger M.

Journal of Physiology and Pharmacology

|

2007

|

tom 58

|

nr 3

Endothelial dysfunction and atherosclerosis are associated with an inflammation-induced decrease in endothelial nitric oxide synthase (eNOS) expression. Based on the differences between hydrophobic and hydrophilic statins in their reduction of cardiac events, we analyzed the effects of rosuvastatin and cerivastatin on eNOS and inducible NO synthase (iNOS) expression and NOS activity in TNF-alpha-stimulated human umbilical vein endothelial cells (HUVEC). Both statins reversed down-regulation of eNOS mRNA and protein expression by inhibiting HMG-CoA reductase and isoprenoid synthesis. Cerivastatin tended to a more pronounced effect on eNOS expression compared to rosuvastatin. NOS activity - measured by conversion of [3H]-L-arginine to [3H]-L-citrulline - was enhanced under treatment with both drugs due to inhibition of HMG-CoA reductase. Statin-treatment reduced iNOS mRNA expression under normal conditions, but had no relevant effects on iNOS mRNA expression in cytokine-treated cells. Rosuvastatin and cerivastatin reverse the detrimental effects of TNF-alpha-induced down-regulation in eNOS protein expression and increase NO synthase activity by inhibiting HMG-CoA reductase and subsequent blocking of isoprenoid synthesis. These results provide evidence that statins have beneficial effects by increasing eNOS expression and activity during the atherosclerotic process.

17

Immunoreactivity of iNOS in porcine uterus after infusions of Escherichia coli endotoxin

75%

Jana B., Andronowska A., Kucharski J.

Folia Histochemica et Cytobiologica

|

2001

|

tom 39

|

nr 2

18

Homocysteine induces endothelial dysfunction via inhibition of arginine transport

75%

Jin L., Caldwell R. B., Li-Masters T., Caldwell R. W.

Journal of Physiology and Pharmacology

|

2007

|

tom 58

|

nr 2

Hyperhomocysteinemia is an independent risk factor for cardiovascular diseases. High levels of plasma homocysteine (HCY) increase oxidative stress and reduce endothelial-dependent relaxation. We determined whether hyperhomocysteinemia-induced endothelial dysfunction is mediated through inhibition of cellular transport of L-arginine. In endothelial cells, HCY had a biphasic effect on arginine transport. HCY treatment for 6 hr increased L-arginine uptake by 34%; however, uptake was decreased by 25% after 24 h. HCY caused membrane hyperpolarization during both 6 and 24 h incubation periods, indicating that the negative charge facilitating arginine uptake was maintained. HCY significantly reduced expression of cellular arginine transporter protein (CAT-1) after 24 h treatment; whereas endothelial nitric oxide synthase (eNOS) protein levels and basal eNOS activity were not altered. Nevertheless, nitric oxide (NO) formation was significantly decreased. The antioxidant ascorbic acid prevented the effect of HCY on arginine transport. HCY induced formation of the peroxynitrite biomarker nitrotyrosine, which was blocked by supplemental L-arginine. HCY treatment of aortic rings caused decreased vasorelaxation to acetylcholine, which was prevented by supplemental arginine. In conclusion, HCY decreased NO formation and induced endothelial dysfunction without altering protein level or basal activity of eNOS, but through decreases in function and protein expression of the CAT-1 transporter. Reduced arginine supply may lead to eNOS uncoupling and generation of superoxide, contributing to HCY-induced oxidative stress.

19

The influence of open field exposure on neurons containing nitric oxide synthase in the basolateral complex and paracapsular intercalated nerve cells of the rat amygdala

75%

Ludkiewicz B., Klejbor I., Domaradzka-Pytel B., Wojcik S., Luczynska A., Badowska-Szalewska E., Dziewiatkowski J., Morys J.

Folia Morphologica

|

2004

|

tom 63

|

nr 4

Our intention in the present study was to ascertain whether NO-producing cells in the basolateral complex (BLC) and paracapsular intercalated nerve cell groups (Ip) of the amygdala are activated in the open field (OF) test. The material consisted of 8 adult rat brains. The OF test was applied throughout 10 min and 90 min before the death of the animals. The brain sections were double stained using the antibodies against c-Fos (marker of neuronal activation) and against nitric oxide synthase (NOS — marker of NO-producing cells). The neurons containing NOS and those revealing c-Fos activity constituted distinct populations within both the BLC and Ip but NOS-immunoreactive fibres often surrounded the c-Fos-immunoreactive neurons. Our results suggest that (1) neurons of the basolateral complex of the amygdala and paracapsular intercalated islands are involved but probably not crucial for the open field stress response and (2) NOS-immunoreactive cells in the BLC and Ip are not activated after OF exposure.

20

Inhibition of NOS-2 induction in LPS-stimulated J774.2 cells by 1,5-isoquinolinediol, an inhibitor of PARP

75%

Olszanecki R., Gebska A., Jawien J., Jakubowski A., Korbut R.

Journal of Physiology and Pharmacology

|

2006

|

tom 57

|

nr 1

Activation of both poly (ADP-ribose) polymerase (PARP) and inducible nitric oxide synthase (NOS-2) have been implicated in the pathogenesis of various forms of inflammation, therefore compounds which may simultaneously inhibit both pathways are of potential therapeutic interest. We tested the influence of potent inhibitor of PARP, 1, 5-isoquinolinediol (ISO), on NOS-2 induction in model of mouse macrophages (cell line J774.2) stimulated with lipopolysaccharide (1 µg/ml). Pretreatment with ISO (1-300 µM) resulted in dose-dependent inhibition of accumulation of NOS-2-derived nitrite in culture medium (IC50 = 9,3 µM) as well as inhibition of NOS-2 protein induction in cultured J774.2 cells; ISO given 10 hours after LPS did not influence activity of NOS-2. Interestingly, another PARP inhibitor, 3-aminobenzamide (3-AB, 10-3000 µM), did not influence 24-hr nitrite accumulation in J774.2 cell culture, either administered 15 minutes prior to LPS or 10 hrs after LPS. Scavenging of reactive oxygen species by use of mixture of SOD and catalase (SOD/Cat, 100/300 - 1000/3000 U/ml) as well as cell permeable SOD-mimetic [Mn(III)TBAP, 1- 100 µM], did not influence NOS-2 induction in J774.2 cells. In summary, we identified 1, 5-isoquinoline as potent inhibitor of induction of NOS-2 in LPS-treated mouse macrophages. The exact mechanism of inhibitory action of this compound on NOS-2 induction requires further investigation.

Ograniczanie wyników

NADPH-diaphorase in porcine uterus after infusions of Escherichia coli endotoxin

An immunohistochemical study on the presence of nitric oxide synthase isoforms (nNOS, iNOS, eNOS) in the spinal cord and nodose ganglion of rats receiving ionising gamma radiation to their liver

The transcriptional cascade associated with creatine kinase down-regulation and mitochondrial biogenesis in mice sarcoma

An ultrastructural demonstration of NADPH-diaphorase-nitric oxide synthase activity in the rat striatal astroglia

Effect of leptin on thyroid-stimulating hormone secretion and nitric oxide release from pituitary cells of ewe lambs in vitro

The expression of inducible nitric oxide synthase [iNOS] in the testis and epididymis of rats with a dihydrotestosterone [DHT] deficiency