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Light and nitrate induction of nitrate reductase in kinetin- and gibberellic acid-treated mustard cotyledons

100%

Saroop S., Thaker V. S., Chanda S. V., Singh Y. D.

Acta Physiologiae Plantarum

1998

tom 20

nr 4

Nitrate reductase activity in gibberellic acid and kinetin treated mustard (Brassica juncea Coss. cv. T-59 ‘Varuna’) seedlings, grown in the presence or absence of light and/or NO₃ was investigated. While both light and NO₃, alone could induce NR activity, their combination showed additive effects. Kinetin treatment significantly promoted both light- and NO₃- induced NR activities, assayed by either in vivo or in vitro techniques, whereas, gibberellic acid was almost ineffective. In the absence of both light and NO₃, however, phytohormones alone could not induce NR activity. Both light-induced and NO₃ induced NR fractions had a pH optima of 7.5, preferred NADH as an electron donor (NADH: NADPH ratio 2.5) and Km values for NO₃ was 0.2 mM. Actinomycin D, cycloheximide and tungstate were equally effective in suppressing the development of NR activity after exposure to light or NO₃. These results indicate that two independent NR fractions operate, with apparently identical properties but separate control mechanisms.

Ograniczanie wyników

1 Acta Physiologiae Plantarum

1 Chanda S. V.

1 Saroop S.

1 Singh Y. D.

1 Thaker V. S.

1 1998

Wyniki wyszukiwania

Light and nitrate induction of nitrate reductase in kinetin- and gibberellic acid-treated mustard cotyledons