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The effect of diabetes during pregnancy on the endocrine pancreas of the newborn has been studied before, but a detailed morphometric immunocytochemical study is not available. The aim of this study was to investigate the effect of maternal diabetes on the morphology of islets in newborn rats. Pancreatic sections were stained by the indirect immunoperoxidase method to localise the insulin-producing β-cells and were used for morphometric analysis. The islet volume density, diameter and volume and the absolute islet cell number were significantly greater in the diabetic group than in controls. The β-cell volume density was significantly lower in the diabetic group, although the β-cell nuclear diameter and volume did not differ significantly in this group. The results obtained from this investigation indicate that maternal diabetes induces islet hypertrophy in newborn offspring and causes an increase in the total islet cell number.
The aim of the investigations was the description of foramen magnum morphology. Fourteen skulls of American Staffordshire terrier pups were examined. The morphology of the foramen magnum was described, its height and width were measured, and the index of the foramen magnum (width/height × 100) was calculated. The height of the skull, maximum width and maximum height of the occipital condyle were also measured. In all the examined skulls the shape of the foramen magnum was regular and ellipsoidal. The average height of the foramen magnum was 5.7 mm, the average width - 6.05 mm. The value of the foramen magnum index was 106.82. The average skull height was 22 mm. The average maximum height of the occipital condyle was 5.33 mm and its average maximum width was 2.85 mm. There were no differences between females and males in all parameters. The shape of the foramen magnum was regular. The occipital condyli were symmetrical.
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