Wyniki wyszukiwania

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Single-electron reduction of quinone and nitroaromatic xenobiotics by recombinant rat neuronal nitric oxide synthase

100%

Anusevicius Z., Nivinskas H., Sarlauskas J., Sari M.-A., Boucher J.-L., Cenas N.

Acta Biochimica Polonica

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2013

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tom 60

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nr 2

We examined the kinetics of single-electron reduction of a large number of structurally diverse quinones and nitroaromatic compounds, including a number of antitumour and antiparasitic drugs, and nitroaromatic explosives by recombinant rat neuronal nitric oxide synthase (nNOS, EC 1.14.13.39), aiming to characterize the role of nNOS in the oxidative stress-type cytotoxicity of the above compounds. The steady-state second-order rate constants (kcat/Km) of reduction of the quinones and nitroaromatics varied from 102 M-1s-1 to 106 M-1s-1, and increased with an increase in their single-electron reduction potentials (E17). The presence of Ca2+/calmodulin enhanced the reactivity of nNOS. These reactions were consistent with an "outer sphere" electron-transfer mechanism, considering the FMNH·/FMNH2 couple of nNOS as the most reactive reduced enzyme form. An analysis of the reactions of nNOS within the 'outer sphere' electron-transfer mechanism gave the approximate values of the distance of electron transfer, 0.39-0.47 nm, which are consistent with the crystal structure of the reductase domain of nNOS. On the other hand, at low oxygen concentrations ([O2] = 40-50 µM), nNOS performs a net two-electron reduction of quinones and nitroaromatics. This implies that NOS may in part be responsible for the bioreductive alkylation by two-electron reduced forms of antitumour aziridinyl-substituted quinones under a modest hypoxia.

2

Blockade of nitric oxide formation in the rat brain does not disturb development of endotoxin tolerance

84%

Soszynski D., Daniluk M., Galazka M., Dmitruk K.

Journal of Physiology and Pharmacology

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2013

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tom 64

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nr 6

3

ST36 electroacupuncture activates nNOS, iNOS and ATP-sensitive potassium channels to promote orofacial antinociception in rats

84%

Almeida R. T., Galdino G., Perez A. C., Silva G., Romero T. R., Duarte I. D.

Journal of Physiology and Pharmacology

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2017

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tom 68

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nr 1

4

Increased expression of CART, nNOS, VIP, PACAP, SP and GAL in enteric neurons of the porcine stomach prepyloric region following hydrochloric acid infusion

84%

Calka J.

Folia Histochemica et Cytobiologica

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2019

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tom 57

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nr 4

5

Rapid blood pressure increase after renal denervation in anaesthetized rats: interaction of oxidative stress and neuronal nitric oxide synthase

84%

Walkowska A., Vaneckova I., Sadowski J., Kompanowska-Jezierska E.

Journal of Physiology and Pharmacology

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2019

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tom 70

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nr 4

6

Expression of nerve growth factor, its TRKA receptor, and several neuropeptides in porcine esophagus. Implications for interactions between neural, vascular and epithelial components of the esophagus

84%

Samarasena J. B., Ahluwalia A., Tarnawski A. S., Shinoura S., Choi K. D., Lee J. G., Chang K. J.

Journal of Physiology and Pharmacology

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2015

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tom 66

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nr 3

7

Intracerebroventricular injection of neuronal and inducible nitric oxide synthase inhibitors does not influence febrile response in rats during turpentine abscess

84%

Soszynski D., Chelminiak M.

Journal of Physiology and Pharmacology

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2007

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tom 58

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nr 4

The purpose of this study was to investigate the role of neuronal nitric oxide synthase (nNOS) and inducible NOS (iNOS) in the brain during development of fever in response to localized tissue inflammation caused by injection of turpentine in freely moving biotelemetered rats. To determine the role of both NOSs in turpentine-induced fever, we injected vinyl-L-NIO (N5 – (1-Imino-3-butenyl) – ornithine (vL-NIO), a selective nNOS inhibitor, and aminoguanidine hydrochloride, a selective iNOS inhibitor, intracerebroventricularly (i.c.v.) 5 h after turpentine injection. Rats responded with fever to intramuscular injection of 20 µl of turpentine that commenced about 5 - 6 h after injection and reached peak value between 9 - 11 h post-turpentine. The inhibition of nNOS as well as iNOS in the brain did not affect fever induced by turpentine. Fevers in control rats (treated i.c.v. with pyrogen-free water) and iNOS or nNOS inhibitor-i.c.v. treated rats injected with turpentine were essentially the same. Furthermore, on the basis of these data, we concluded that iNOS and nNOS inside the brain do not participate in generation of fever to turpentine in rats.

8

Neuronal nitric oxide synthase phosphorylation induced by docosahexaenoic acid protects dopaminergic neurons in an experimental model of Parkinson’s disease

67%

Parlak H., Ozkan A., Dilmac S., Tanriover G., Ozsoy O., Agar A.

Folia Histochemica et Cytobiologica

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2018

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tom 56

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nr 1

9

Blood pressure-independent hypotrophy of the heart, kidneys and conduit arteries after 7-nitroindazole administration to Wistar rats from the prenatal period to adulthood

67%

Kristek F., Malekova M., Ondrias K., Cacanyiova S.

Journal of Physiology and Pharmacology

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2013

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tom 64

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nr 1

10

Chronic stress adaptation of the nitric oxide synthases and IL-1beta levels in brain structures and hypothalamic-pituitary-adrenal axis activity induced by homotypic stress

67%

Gadek-Michalska A., Tadeusz J., Rachwalska P., Bugajski J.

Journal of Physiology and Pharmacology

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2015

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tom 66

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nr 3

11

Effect of chronic neuronal nitric oxide-synthase inhibition on arterial function and structure in spontaneously hypertensive rats

67%

Cacanyiova S., Kristek F., Malekova M., Ondrias K.

Journal of Physiology and Pharmacology

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2012

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tom 63

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nr 1

Ograniczanie wyników

Single-electron reduction of quinone and nitroaromatic xenobiotics by recombinant rat neuronal nitric oxide synthase

Blockade of nitric oxide formation in the rat brain does not disturb development of endotoxin tolerance

ST36 electroacupuncture activates nNOS, iNOS and ATP-sensitive potassium channels to promote orofacial antinociception in rats

Increased expression of CART, nNOS, VIP, PACAP, SP and GAL in enteric neurons of the porcine stomach prepyloric region following hydrochloric acid infusion

Rapid blood pressure increase after renal denervation in anaesthetized rats: interaction of oxidative stress and neuronal nitric oxide synthase

Expression of nerve growth factor, its TRKA receptor, and several neuropeptides in porcine esophagus. Implications for interactions between neural, vascular and epithelial components of the esophagus

Intracerebroventricular injection of neuronal and inducible nitric oxide synthase inhibitors does not influence febrile response in rats during turpentine abscess

Neuronal nitric oxide synthase phosphorylation induced by docosahexaenoic acid protects dopaminergic neurons in an experimental model of Parkinson’s disease

Blood pressure-independent hypotrophy of the heart, kidneys and conduit arteries after 7-nitroindazole administration to Wistar rats from the prenatal period to adulthood

Chronic stress adaptation of the nitric oxide synthases and IL-1beta levels in brain structures and hypothalamic-pituitary-adrenal axis activity induced by homotypic stress

Effect of chronic neuronal nitric oxide-synthase inhibition on arterial function and structure in spontaneously hypertensive rats