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Hydrogen sulfide modulates gastric acid secretion in rats via involvement of substance P and nuclear factor-kappaB signaling

100%

Sun H.-Z., Gong X.-Y., Wu L., Wang X.-X., Nie Y.-N., Shang R., Wang H., Li Y.-C., Sun Q.-F., Gao P.-F., Bi J.-X.

Journal of Physiology and Pharmacology

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2018

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tom 69

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nr 3

2

The antiproliferative action of [D-Arg1, D-Phe5, D-Trp7,9, Leu11] substance P analogue antagonist against small cell- and non-small-cell lung cancer cells could be due to the pharmacological profile of its tachykinin receptor antagonist

84%

Munoz M., Recio S., Rosso M., Redondo M., Covenas R.

Journal of Physiology and Pharmacology

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2015

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tom 66

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nr 3

3

Establishing and functional characterization of an HEK-293 cell line expressing autofluorescently tagged beta-actin [pEYFP-actin] and the neurokinin type 1 receptor [NK1-R]

84%

Hrovat A., Zavec A. B., Pogacnik A., Frangez R., Vrecl M.

Cellular and Molecular Biology Letters

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2010

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tom 15

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nr 1

55-69

This study focused on establishing and making a comprehensive functional characterization of an HEK-293-transfected cell line that would coexpress the enhanced yellow fluorescent protein-actin (pEYFP-actin) construct and the neurokinin type 1 receptor (NK1-R), which is a member of the seven transmembrane (7TM) receptor family. In the initial selection procedure, the cloning ring technique was used alone, but failed to yield clones with homogenous pEYFP-actin expression. Flow cytometry sorting (FCS) was subsequently used to enrich the pEYFP-actin-expressing subpopulation of cells. The enzyme-linked immunosorbent assay (ELISA), FCS and quantitative real-time reverse transcription/polymerase chain reaction (RT-PCR) were then employed to monitor the passage-dependent effects on transgene expression and to estimate the total β-actin/pEYFP-actin ratio. NK1-R was characterized via radioactive ligand binding and the second messenger assay. The suitability of the pEYFP-actin as a marker of endogenous actin was assessed by colocalizing pEYFP-actin with rhodamine-phalloidine-stained F-actin and by comparing receptor- and jasplakinolide-induced changes in the actin cytoskeleton organization. These experiments demonstrated that: i) both constructs expressed in the generated transfected cell line are functional; ii) the estimated pEYFP-actin: endogenous β-actin ratio is within the limits required for the functional integrity of the actin filaments; and iii) pEYFP-actin and rhodamine-phalloidine-stained F-actin structures colocalize and display comparable reorganization patterns in pharmacologically challenged cells.

4

Antimicrobial peptides in the brain

59%

Su Y., Zhang K., Schluesener H. J.

Archivum Immunologiae et Therapiae Experimentalis

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2010

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tom 58

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nr 5

365-377

Ograniczanie wyników

2 Journal of Physiology and Pharmacology

1 Archivum Immunologiae et Therapiae Experimentalis

1 Cellular and Molecular Biology Letters

1 Bi J.-X.

1 Covenas R.

1 Frangez R.

1 Gao P.-F.

1 Gong X.-Y.

1 Hrovat A.

1 Li Y.-C.

1 Munoz M.

1 Nie Y.-N.

1 Pogacnik A.

1 Recio S.

1 Redondo M.

1 Rosso M.

1 Schluesener H. J.

1 Shang R.

1 Su Y.

1 Sun H.-Z.

1 Sun Q.-F.

1 Vrecl M.

1 Wang H.

1 Wang X.-X.

1 Wu L.

1 Zavec A. B.

1 Zhang K.

1 2018

1 2015

2 2010

Hydrogen sulfide modulates gastric acid secretion in rats via involvement of substance P and nuclear factor-kappaB signaling

The antiproliferative action of [D-Arg1, D-Phe5, D-Trp7,9, Leu11] substance P analogue antagonist against small cell- and non-small-cell lung cancer cells could be due to the pharmacological profile of its tachykinin receptor antagonist

Establishing and functional characterization of an HEK-293 cell line expressing autofluorescently tagged beta-actin [pEYFP-actin] and the neurokinin type 1 receptor [NK1-R]

Antimicrobial peptides in the brain