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À complex, cascaded neural network designed to predict the secon­dary structure of globular proteins has been developed. Information about the local buried-unburied pattern and the average tendency of the particular types of amino acids to be buried inside the globule were used. Nonspecific information about long distance contact maps was also employed. These modifications result in a noticeable improvement (3 - 9%) of prediction accuracy. The best result for the average success ratio for the testing set of nonhomologous proteins was 68.3% (with corresponding Matthews' coefficients, C
We present here a neural network-based method for detection of signal peptides (abbreviation used: SP) in proteins. The method is trained on sequences of known signal peptides extracted from the Swiss-Prot protein database and is able to work separately on prokaryotic and eukaryotic proteins. A query protein is dissected into overlapping short sequence fragments, and then each fragment is analyzed with respect to the probability of it being a signal peptide and containing a cleavage site. While the accuracy of the method is comparable to that of other existing prediction tools, it provides a significantly higher speed and portability. The accuracy of cleavage site prediction reaches 73% on heterogeneous source data that contains both prokaryotic and eukaryotic sequences while the accuracy of discrimination between signal peptides and non-signal peptides is above 93% for any source dataset. As a consequence, the method can be easily applied to genome-wide datasets. The software can be downloaded freely from http://rpsp.bioinfo. pl/RPSP.tar.gz.
Nowadays overwhelming majority of biotechnical objects in agriculture, such as poultry houses, greenhouses etc., function under the mode of stabilization of technological parameters (air temperature, humidity etc.). This approach leads to excess consumption of energy resources (electrical energy, natural gas). Intelligent control based on using different strategies (not only stabilization), prediction and consideration of natural disturbances on biotechnical objects, physiological features of biological objects (poultry, plants etc.) allows to reduce energy consumption. The paper presents specific knowledge concerning promising areas of control systems of biotechnical objects, methodological bases for specialized algorithmic-mathematical software construction based on the methods of game theory and statistical solutions, neural networks (including genetic algorithm), filtering the noise components of information signals.
This paper describes the application of artificial neural networks (ANNs) for the time series modeling of total phosphorous concentrations in the Odra River. Data from the monitoring site Police in the lower part of the Odra were used for training, validating and testing the models. Two models are proposed to prove the satisfactory forecast of phosphorus concentrations: a simpler one with a single input variable and a more complex one with 14 input variables. Both ANN models show a high ability to predict from the new data set. On the basis of sensitivity analysis the relationships between phosphorus concentrations and other water quality variables were established.
Erythropoietin is a potent regulator of erythropoiesis. It acts via the specific membrane receptor (EpoR). Erythropoietin is also known to be present in the central nervous system, and its concentration and the expression of EpoR change during development, which raises the possibility that this modulator might be involved in the regulation of neuronal functions in the developing brain. The GABAergic system undergoes profound changes during development and is particularly susceptible to modulation by endogenous factors. Therefore, we decided to investigate the impact of Epo on GABAergic transmission in hippocampal neurons developing in vitro. An analysis of miniature IPSCs (mIPSCs) revealed that a long-term treatment with Epo (48 or 72 h) resulted in a major acceleration of the decaying phase of these currents while the amplitude and current frequency remained unchanged. Interestingly, this effect was restricted to the youngest considered age group (6-8 DIV), indicating that Epomediated modulation of mIPSCs depends on the developmental stage of the neurons. We conclude that Epo may exert a modulatory action on GABAergic transmission in developing neural networks.
The high-performance liquid chromatography (HPLC) procedure based on gradient elution technique was used to separate flavonoids in leaves of Taxus baccata var. elegantissima and Metasequoia glyptostroboides. Optimization of chromatographic separations was supported by artificial neural networks. The best gradient conditions acquired to separate analyzed compounds were established and then used in experiments. Predictive errors were additionally calculated. Satisfactory correlation between predicted and experimental retention data was obtained.
Engineering geodesy deals with a wide range of problems. There is also a part that deals with measuring displacements and deformations of engineering objects. Correct geodetic monitoring requires identifying the movement of points representing an engineering object in order to determine displacement values, taking into account the time function. The paper presents the results of research on kinematic models of geodetic networks in the aspect of using them for describing the state of vertical displacements of engineering objects located on expansive soil. The paper presents two functional models of an observation system: one in the form of a second rank polynomial and the other in the form of an exponential function. The selected kinematic models of measurement-control geodetic networks were estimated with classic methods and neural networks.
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