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Photosensitizing dyes are used in fluorescence diagnostics and photodynamic ther­apy (PDT). These usually hematoporphyrin derivatives (HpD) accumulate preferen­tially within neoplastic tissues. HpD is a mixture of ether and ester linked porphyrins. Its partially purified form is known under the commercial name of photofrin II (PII). PII emission spectra were studied in a hydrophilic (PBS) and a lipophilic (PC liposomes) environment. Red shift was observed in their emission maxima from 615 nm in buffer solution to 635 nm in lipid. Identical red shift was obtained when the intracellular fluorescence of two cancer cell lines, MCF 7 and Jurkat, incubated with PII was investigated. Thus, intramembrane localization of PII may be suggested. As determined from the total fluorescence intensity, the uptake of PII was only slightly higher for Jurkat than for MCF 7 cells. Nevertheless the kinetics of the uptake was found to be different for both cell lines.
This paper presents results of a study on determination of the concentration of Cd, Pb, Cu and Zn in neoplastic and peri-neoplastic lung tissues from 110 subjects of two regions of different degrees of environmental pollution: Wielkopolska (75) and Upper Silesian Industrial District (35). The concentrations of the metals were determined by the method of differential pulse anodic stripping voltammetry (DPASV). The mean values (in mug/g wet tissue) obtained for the inhabitants from Wielkopolska were: Cd - 0.56; Pb - 0.45; Cu - 1.98; Zn - 12.93 in the neoplastic tissue and Cd - 0.88; Pb - 0.40; Cu - 1.96; Zn - 12.80 in the peri-neoplastic tissue. For the inhabitants from the Upper Silesian Industrial District (USID) the corresponding values were: Cd - 0.63; Pb - 0.25; Cu - 1.61; Zn - 20.63 in the neoplastic tissue and Cd - 1.37; Pb - 0.06; Cu - 1.66; Zn - 19.20 in the peri-neoplastic tissue. Concentrations of Cd were higher in peri-neoplastic tissues than in the neoplastic ones in the subjects from both groups. The highest concentrations of Pb and Cu were found in tissues of the inhabitants from Wielkopolska, while the highest concentrations of Cd and Zn were in tissues of those from Upper Silesian Industrial District.
Zinc and copper concentrations were assessed in skin tumours and serum of 26 dogs of different breeds. The analysed tumours were situated on legs, chest, and abdomen, 8, 7, and 5 cases, respectively. In 2 cases, the tumours were situated on the tail, anal region, and head. On the basis of a histopathological examination, 17 tumours were classified as malignant, while 9 were classified as benign ones. The dogs age-matched to those with neoplastic changes belonged to the control group. However, they were subjected to surgery due to reasons other than tumours. Samples of skin with subcutaneous tissue and serum obtained from these animals were handled in an identical procedure to those of the other groups. The spectrophotometric atomic absorption method and PAY-UNICAM apparatus were used to determine zinc and copper concentrations. It was demonstrated that the concentration of zinc and copper in malignant tumours was significantly increased in relation to the tissues obtained from healthy animals. Furthermore, tissue concentration of copper in benign tumours was significantly increased in comparison to the controls. In conclusion, the determination of zinc and copper concentrations in malignant neoplastic tissues and serum of dogs may be used as an additional diagnostic indicator conforming histopathological examination of tumour malignancy.
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