Wyniki wyszukiwania

1

The influence of modification at position 2 on the side-chain conformation in oxytocin analogs

100%

Smiech B., Kazmierkiewicz R., Lammek B.

Acta Biochimica Polonica

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2006

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tom 53

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nr 1

The nonapeptide oxytocin (OT) is used in medicine to help begin and/or continue childbirth. Its analogs can be also used to control bleeding following fetus delivery. The main function of oxytocin is to stimulate contraction of uterus smooth muscle and the smooth muscle of mammary glands, thus regulating lactation. This paper describes theoretical simulations of the distribution of the torsional angles χ1 in the non-standard methylated phenylalanine residues of three oxytocin analogs: [(Phe)2o-Me]OT, [(Phe)2m-Me]OT, [(Phe)2p-Me]OT. The conformations of the oxytocin analogs were studied both in vacuum and in solution. We found some correlations between the biological activity of the considered peptides and the side-chain conformations of amino-acid residues 2 and 8.

2

Isolation and biological characterisation of a new isolate of Neospora caninum from an asymptomatic calf in Brazil

63%

Garcia-Melo D. P., Regidor-Cerrillo J., Ortega-Mora L. M., Collantes-Fernandez E., Ferreira de Oliveira V. S., Pelli de Oliveira M. A., Caetano da Silva A.

Acta Parasitologica

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2009

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tom 54

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nr 2

180-185

Neospora caninum is a tissue-cyst forming parasite that has been recognized worldwide as a cause of abortion in cattle. Despite the ubiquitous distribution of this parasite and its broad range of hosts, the number of N. caninum isolates obtained to date is limited. In addition, the majority of isolates have been obtained from clinically affected hosts, therefore potentially biasing this population towards more virulent isolates. This report describes the isolation and biological characterisation of a new N. caninum isolate, Nc-Goiás 1, obtained from an asymptomatic, naturally infected calf from Brazil. This new isolate was identified as a member of the N. caninum species by polymerase chain reaction (PCR) using specific primers based on the N. caninum internal transcribed spacer 1 (ITS-1) sequence, and was genetically identified at multiple loci using microsatellite analysis. Finally, a pathogenicity study was conducted in a BALB/c mice model. All Nc-Goiás 1-infected mice survived without exhibiting any clinical signs. Further pathogenic characterisation of this isolate suggested that Nc-Goiás 1 is less virulent than other N. caninum isolates (Nc-Liv and Nc-1) studied in this mouse model. This is the first report of the isolation and biological characterisation of N. caninum from an infected but clinically healthy calf in South America.

3

Comparative study of selected blood biochemical components in milk or milk-replacer fed calves during the second week of life

51%

Lepczynski A., Herosimczyk A., Dratwa-Chalupnik A., Ozgo M., Michalek K., Malinowski E., Skrzypczak W. F.

Folia Biologica

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2011

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tom 59

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nr 3-4

4

Shifts in immunoglobulins levels in the porcine mammary secretions during whole lactation period

51%

Markowska-Daniel I., Pomorska-Mol M.

Bulletin of the Veterinary Institute in Puławy

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2010

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tom 54

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nr 3

The absolute and relative levels of immunoglobulins (Ig) G, M, and A in porcine mammary secretions throughout lactation period were estimated. Concentrations of each immunoglobulin were determined by ELISA kits. The results of the study confirmed that pigs, like other mammals, have colostral and milk phases of lactation, distinguished by the concentrations of IgG and IgA. The amount of IgG showed a dramatic 27-fold decline from the beginning to day 6 of lactation. The IgM concentration decreased over threefold at the same time. Concentration of IgA at the day 6 of lactation was almost 4-fold lower than that at the beginning of lactation. The relative contribution of IgG and IgA to the total Ig content becomes reversed during lactation. Summarising, in pigs, transformation of colostrum to milk was characterised by dramatic decrease in total Ig content, connected with the change of predominant immunoglobulin. The predominant immunoglobulin in colostrum was IgG, which plays an important role in systemic immunity, while in milk a major Ig was IgA, responsible for the protection at mucosal surface level. As concentrations of Ig in colostrum are very variable, an improved understanding of factors influencing colostrum Ig concentration is now desirable.

5

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Neosporosis and toxoplasmosis in South American camelids from Peru

51%

Serrano-Martinez E., Chavez-Velasquez A., Collantes-Fernandez E., Alvarez-Garcia G., Casas-Astos E., Rosadio-Alcantara R., Risco-Castillo V., Ortega-Mora L. M.

Wiadomości Parazytologiczne. Suplement

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2005

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tom 51

6

Ultrasound diagnostic schema for the determination of increased risk for chromosomal fetal aneuploidies in the first half of pregnancy

51%

Sieroszewski P., Perenc M., Bas-Budecka E., Suzin J.

Journal of Applied Genetics

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2006

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tom 47

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nr 2

The aim of the study was to develop an early ultrasound diagnostic schema for the determination of increased risk for fetal chromosomal aneuploidies. The study was conducted on a population of 1318 pregnant women divided into 2 groups: 1255 women with the normal course of pregnancy and 63 women with diagnosed fetal abnormalities. There were 34 cases of chromosomal abnormalities (trisomy 21,18,13; triploidy; unbalanced inversion 9; deletion 16) and 29 cases of structural malformations. The estimation of the range of normal values was performed for the nuchal translucency (NT) measurement between 11 and 13 weeks and the nasal bone length (NB) measurement between 12 and 20 week. The results obtained in the collective set of normal pregnancies constituted the basis for the calculation of the range of normal values. The measurements of NB and NT showed a linear value increase with the pregnancy course. The following test characteristics (correlation to CRL) were recorded: NB - sensitivity 60%, specificity 98%, positive predictive value (PPV+) 43%, negative predictive value (NPV-) 98.9%. For the assumption that the test outcome means the presence or absence of the nasal bone in the ultrasound scan the sensitivity was 40%, but specificity 100%; NT - sensitivity 63.6%, specificity 98.2%, PPV+ 38.9%, NPV - 98.2%; NT + NB - presents similar characteristic to the NB or NT alone - sensitivity 55.6%, specificity 98.6%, PPV+ 50%, NPV - 98.9%. The following test characteristics for chromosomal aberration markers (correlation to BPD) were observed: NB - sensitivity 68.4%, specificity 97.4%, PPV+ 56.5%, NPV - 98.4%; NT - sensitivity 73.9%, specificity 97.9%, PPV+ 54.8%, NPV- 99.2%; NT + NB - sensitivity 94.7%, specificity 98.9%, PPV+ 90%, NPV - 99.7%, respectively. The “genetic sonogram” protocol for the structural defect detection was analysed: sensitivity was 80%, specificity 100%, PPV+ 100%, NPV - 99.7%. It is concluded that the new biometric parameter - nasal bone length (NB) and the corrected one - nuchal translucency thickness (NT) are useful markers for fetal abnormalities, especially for chromosomal aberrations. High predictive values of the diagnostic schema for the detection of aneuploidies and structural defects indicate that its application in correlation with the biparietal diameter (BPD) is highly recommended. The proposed schema is an effective algorithm for prenatal diagnostics characterised by high prognostic values. The possible introduction of the schema could result in a decrease of the invasive procedure rates, which could minimise the rate of miscarriages as a complication of amniocenteses.

Ograniczanie wyników

The influence of modification at position 2 on the side-chain conformation in oxytocin analogs

Isolation and biological characterisation of a new isolate of Neospora caninum from an asymptomatic calf in Brazil

Comparative study of selected blood biochemical components in milk or milk-replacer fed calves during the second week of life

Shifts in immunoglobulins levels in the porcine mammary secretions during whole lactation period

Neosporosis and toxoplasmosis in South American camelids from Peru

Ultrasound diagnostic schema for the determination of increased risk for chromosomal fetal aneuploidies in the first half of pregnancy