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Experimental model in ovo. Glioblastoma multiforme (GBM) is the most common primary malignancy in the brain and confers a uniformly poor prognosis. Despite decades of research on the topic, limited progress has been made to improve the poor survival associated with this disease, new therapeutic strategies are still needed. The application of nanotechnology to disease treatment, diagnosis, monitoring, drug delivery platform and to the control of biological systems is promising, also in cancer therapy. Diamond na-noparticles (DN) are bioactive substance toward glioma tumour cultured on the chicken embryo chorioallantoic membrane (CAM). DN reduce tumor mass and volume and inhibited new blood vessel development in glioma tumor. In the present experiment we additionally observed, that DN caused changes in the tumor ultrastructure testify to the ongoing process of cell death, probably carried out by autophagy.
Interaction of hierarchical nanoporous carbons(HNCs) with chicken embryo red blood cells (RBC.The purpose of this study was to characterize toxicity of hierarchical nanoporous carbons (HNCs) on chicken embryo red blood cells (RBC). RBC are a perfect model to adapt the hemolysis assay in evaluation of the in vitro blood compatibility of nanoparticles. The samples of blood were treated with different concentration of HNCs (10 µg/ml, 50 µg/ml and 100 µg/ml). Hemolysis assay showed that the hemolytic activity depends on the dose of HNCs. The microscope observations have shown a difference in morphology between treated and untreated RBC: changes in cell membrane shape and the occurrence of pathological erythrocytes forms.
The discovery of cyclosporine A was a milestone in organ transplantation and the treatment of autoimmune diseases. However, developing an efficient oral delivery system for this drug is complicated by its poor biopharmaceutical characteristics (low solubility and permeability) and the need to carefully monitor its levels in the blood. Current research is exploring various approaches, including those based on emulsions, microspheres, nanoparticles, and liposomes. Although progress has been made, none of the formulations is flawless. This review is a brief description of the main pharmaceutical systems and devices that have been described for the oral delivery of cyclosporine A in the context of the physicochemical properties of the drug and the character of its interactions with lipid membranes.
The recent advances in nanotechnology and its use in the field of agriculture are astonishingly increasing; therefore, it is important to understand their role in plant life. Four wheat cultivars soaked in different concentrations of TiO2-NPs (0.0%, 0.025 %, 0.05 %, 0.1 %, 0.2 % and 0.5 %) to select a concentration that stimulate cultivars growth under normal conditions during germination stage. Cultivar dependency appeared markedly in their response to TiO2-NPs. Generally, TiO2-NPs did not modify germination percentage, despite 0.1 % TiO2-NPs vastly enhanced seed potential by increasing vigor index, root dry matter stress tolerance index, shoot dry matter stress tolerance index, dry matter stress tolerance index, plant height stress tolerance index, root length stress tolerance index, fresh matter stress tolerance index and pigment composition. The surrounding concentrations exert little effect on the studied parameters and 0.5 % TiO2-NPs suppressed all indices.
Obtaining of metallic nanoparticles by plasma-erosion electrical discharges in liquid mediums for biological application. The process of electrical discharge in the water of the plasma discharge channel was investigated and its diagnostics was performed. The specific features of the energy redistribution were shown, together with the physical model of the dispersed phase. The biological functionality of the metal nanoparticles and the prospects for their use in biotechnology were found.
Biological method is considered as eco-friendly and reliable process for the synthesis of silver nanoparticles (AgNps) in the field of nanotechnology due to its tremendous applications in various fields. In this study we have isolated a total of twelve endophytic fungi from leaves of Curcuma longa (turmeric) and Catharanthus roseus out of which six endophytic fungi showed their ability to synthesized AgNps from silver nitrate (AgNO3) solution which splits into a positive silver ion (Ag+) and a negative nitrate ion (NO3 -) in order to turn the silver ions into solid silver (Agº). Of the six positive endophytic fungi VRD2 showed good and encouraging results and was identified as Penicillium spinulosum VRD2. UV-Visible Spectroscopy confirms the AgNps showing maximum peak at 425nm implying the bioreduction of AgNO3. Transmission Electron Microscopy (TEM) revealed the particle are spherical and well dispersed without agglomeration size ranging from 25- 30nm.
This study was done to assess the antifungal effect of some biocontrol agents effective microorganisms (EMs1), Pseudomonas fluorescences, and Bacillus pumilus, titanium dioxide (TiO2) nanoparticles, black cumin and wheat germ oils as well as the recommended fungicide (flutolanil) against root rot of tomato. Moreover, gas chromatography-mass spectrometry (GC-MS) examination was completed to identify the bioactive compounds in plant oils (dark cumin and wheat germ). Also the impact of these medicines on some biochemical and growth parameters of tomato was examined. Flutolanil was the best treatment followed by dark cumin, TiO2, EMs1, Pseudomonas fluorescens, Bacillus pumilus and wheat germ oil, individually in both test seasons. The outcomes demonstrated a marked increase in each biochemical character (chlorophyll substance, peroxidase and polyphenoloxidase) and plant development (height and fresh and dry weight) under all the tried treatments in comparison to the controls.
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