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The genus Scopulariopsis contains over 30 species of mitosporic moulds, which although usually saprophytic may also act as opportunistic pathogens in humans. They have mainly been associated with onychomycosis, and only sporadically reported as a cause of deep tissue infections or systemic disease. Identification of Scopulariopsis species still largely relies on phenotype-based methods. There is a need for a molecular diagnostic approach, that would allow to reliably discriminate between different Scopulariopsis species. The aim of this study was to apply sequence analysis of partial 28S rRNA gene for species identification of Scopulariopsis clinical isolates. Although the method employed did reveal some genetic polymorphism among Scopulariopsis isolates tested, it was not enough for species delineation. For this to be achieved, other genetic loci, within and beyond the rDNA operon, need to be investigated.
Current issues concerning Parasitology and Mycology with regard to diseases of the skin and its appendages are presented. Aspects of diagnostics, clinical picture and therapy of skin and nail mycoses, as well as difficulties in the diagnosis and treatment of both native parasitoses (toxoplasmosis) and imported human tropical parasitoses (malaria, filariosis) have been emphasised. The clinical importance of environmental mould fungi in nosocomial infections and fungal meningitis, as well as selected properties of fungi isolated from patients with head and neck neoplasms treated by radiotherapy are discussed. Other mycological topics include the characteristics of newlysynthesized thiosemicarbazides and thiadiazoles as potential drugs against toxoplasmosis and their biological activity against Toxoplasma gondii tachyzoites, selected molecular mechanisms of resistance to azoles, Candida albicans strains and a new tool (barcoding DNA) for describing the biodiversity of potential allergenic molds. The importance of environmental factors in pathogenesis of mycoses and parasitoses is noted. The characteristics of pathogenic fungi isolated from natural ponds in Bialystok and potentially pathogenic yeast-like fungi isolated from children’s recreation areas in Lodz are presented. The ongoing problem of anthropozoonoses is considered, as are the roles of stray cats and dogs in contaminating soil with the developing forms of intestinal parasites. The characteristics of the human microbiome, including population composition, activity and their importance in normal human physiology, are presented, as are the major goals of the Human Microbiome Project initiated by National Institutes of Health (NIH).
The aim of the presented mini-review is to review the literature data referring to opportunistic mycoses in pet dogs and cats suffering from other concurrent diseases, comparable to human medical disorders with high risk of secondary mycoses. This review also presents the preliminary results of a project aimed at understanding the fungal colonization and occurrence of secondary mycoses in pets suffering from metabolic disorders, neoplasms and viral infections. The incidence of opportunistic mycoses is higher in such individuals, mostly because of their impaired immunity. The main risk factors are primary and secondary types of immunodeficiency connected with anti-cancer treatment or neoplastic disease itself. Moreover, literature data and the results of our investigations show that Candida yeasts are prevalent among diabetic animals and indicate that these fungi are the main etiological agents of secondary infections of the oral cavity, GI and urogenital tracts. Other important conditions possibly favoring the development of mycoses are concurrent infections of cats with FeLV and FIV viruses. Thus, in all cases of the mentioned underlying diseases, animals should be carefully monitored by repeated mycological examination, together with inspection of other parameters. Also, the prophylaxis of opportunistic mycoses should be carefully considered alike other factors influencing the prognosis and the outcome of primary diseases.
In the years 1997-1999 the occurrence of rye diseases in the region of south-western Poland was investigated on rye varieties Warko, Dańkowskie Złote and hybrid rye Marder. Eyespot (Pseudocercosporella herpotrichoides var. acuformis) and brown rust (Puccinia recondita) occurcd most frequently in the experimental period. Fusarium stem base rot and Ieaf blight (Fusarium spp.) and leaf scald (Rhynchosporium secalis) were occasionally of considerable importance, but their occurrence was usually less frequent. Variety Marder showed high susceptibility to stem base and leaf diseases, especially to brown rust. When one treatment with fungicides was applied, grain yield increase of cv Marder reached the value of 20,03% in conditions of high infection by pathogens. lt was concluded that in the years favourable for the development of diseases on rye, chemical control with broad-spectrum fungicides might be advisable, especially for protecting susceptible varieties.
In 2006, an emaciated female peregrine falcon (Falco peregrinus) was found in Szczecin (North-West Poland). Parasitological, microbiological, and ecotoxicological (including determination of concentrations Mn, Fe, Cu, Zn, Ni, V, Pb, and Cd) examinations involved the digestive tract, kidneys, liver, brain, breast muscle, and bone. The presence of nematode Serratospiculum tendo (in air sacs), trematodes Strigea falconi and Conodiplostomum spathula, and cestode Cladotaenia cylindracea (in the digestive tract) were demonstrated. Two species of fungi (Aspergillus nidulans and Cladophialophora boppi), responsible for mycosis, were isolated from the skin of breast region. Escherichia coli (rough type) was isolated from the liver, kidneys, lung, small intestine, and heart. Moreover, Micrococcus luteus and Proteus mirabilis were found in the lungs. Worrisome high concentrations of Fe and Mn were denoted, especially in the liver (over 6 000 and 22 mg/kg d.w., respectively).
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