Effect of Trichoderma isolates on mycelium development of some strains of Agaricus bisporus was investigated. The following strains of A. bisporus were examined: Amycel 2000, Polmycel 22, Irlandzka 501, Sylvan 130, Le Lion X13 and Somycel 516. The Trichoderma isolates belonged to several species, i.e. T. aggressivum f. europaeum, T. atroviride, T. harzianum, T. hamatum, T. inhamatum, T. koningii and T. viride. The experiment was conducted on wheat agar medium. Based on individual biotic effect (IBE) varied interactions between Trichoderma isolates and A. bisporus strains were found. The obtained IBE index ranged from +2 to +8. The highest IBE index (+8) was recorded for T. aggressivum f. europaeum T. agg. 2/37 isolate in relation to all examined A. bisporus strains. The IBE index for T. harzianum T. har. 3/48 and T. viride. T. vir. 4/29 isolates reached the value from +5 to +6. The IBE index for T. hamatum T. ham. 1/22 isolate ranged from +3 to +4, while for T. inhamatum T. inh. 5/32 isolate fluctuated from +2 to +4.