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The economical efficiency of the application of an increasing hydrogel dose in cultivar mushrooms (Agaricus bisporus (Lange) Sing. Imbach) has been experimented in controlled weather conditions. It was found that the application of a 50g·m-2 hydrogel dose is not economically proved due to the lack of yield significant increase. In the cultivation of this particular variety of mushrooms, the optimal hydrogel dose shouldn’t be low than 100 g·m2.
Substrate yield-forming properties and their impact on common mushroom yields were evaluated in the course of the performed experiments. All substrates for mushroom cultivation were characterised by considerable variability of yield-forming characteristics. The following yield-forming factors in mushroom cultivation were analysed: weight of substrate in kg·m-2 of cultivation area, the method of substrate preparation, substrate moisture content at filling of the cultivation chamber, amount of substrate dry matter in kg·m-2 of cultivation area, ammonia concentration in phase II substrate after pasteurisation. The highest mushroom yields were obtained from phase III substrate. An increase of the substrate dry matter per square metre of cultivation area by 1% led to a significant increase in the yield of mushrooms.
Edible mushrooms (Agaricus bisporus) were enriched with an irrigation solution containing or not fortified with selenium in form of sodium selenite. Se in control mushrooms was 1.04 mcg/g D.M. and in mushrooms subjected irrigations with water solution of Se this increased to 15 mg of Se/dm) after the first flush and rose to with subsequent flushes to 30.14 mcg/g D.M. Peroxidase activity offresh mushrooms was reduced in Se-enriched Agaricus bisporus. Postharvest storage Peroxidase activity increase for 6 days and then a depression in activity .occutred. A positive correlation between peroxidase activity and fruit body mass of mushrooms was observed. Following fractionation on Sephadex G-100 column 4 peaks of peroxidases activity was observed in water extracts of mushrooms regardless of Se treatment. Molecular mass of these protein fractions were estimated by molecular mass markers as 82, 57, 45 and 22 kDa. Substantial part of peroxidase activity appears to originated from Se-dependent glutathione peroxidase.
Sporophores from five species of Lactarius mushrooms had a liquid rubber content of 0.1 % to 7% based on the dry weight. Rubber from L. voleinus, L. chrysorrheus and L. hygrophoroides was found to be a homologue of polyprenol being composed of dimethylallyl group, two trans isoprene units, 160-300cis isoprene units, and terminal hydroxy! or ester group aligned in that order by 13C-NMR analysis. The ratio of fatty acid ester group to hydroxyl group was about 9/1 to 5/5. The number of both terminal groups and trans units decreased during aging of sporophores. Rubber from L. piperatus, L. vellereus and L. subpiperatus was found to be cis polyisoprene having very small quantities of both terminal groups and trans units. The biosynthesis of cis polyisoprene in Lactarius mushrooms was found to start from trans, trans-famesyl pyrophosphate. The termination was assumed to occur by esterification of polyisoprenyl pyrophosphate. Occurrence of some chemical modifications on both terminal groups was presumed during aging of sporophores.
Some mechanical properties of fresh and freeze-dried mushrooms have been described. Modulus of elasticity was determined from the squeezing tests. The set quantities have been related to the heating plate temperature and density of samples. The obtained relationships have been approximated with exponential functions.
Hericium erinaceum (Bull.: Fr.) Pers. is an edible fungus of great significance in medicine. It is rarely found in Europe, in contrast, it is common in Japan and North America. Its fruitbodies have been well-known for hundreds of years in traditional Chinese medicine and cuisine. A cradle of H. erinaceum cultivation is Asia. In Eastern Europe is rare in natural habitats, but can be successfully cultivated. Both fruitbodies and mycelia are rich in active, health promoting substances. Tests of substances extracted from this mushroom carried out on animals and in vitro have given good results. They can be used in the treatment of cancer, hepatic disorders, Alzheimer’s and Parkinson’s diseases, wound healing. They improve cognitive abilities, support the nervous and immune systems. Promising results have been reported in clinical trials and case reports about the human treatment (e.g., recovery from schizophrenia, an improvement of the quality of sleep, alleviation of the menopause symptoms). The subject of this paper is to summarize information about the development of mycelium, the best conditions for cultivation of fruitbodies, bioactive substances and their use in medicine.
The aim of the research was to determine the effect of medium on the mycelium growth of L. sulphureus. The subject of the studies were six L. sulphureus strains: LS02, LS206, LS286, LS302, LSCNT1 and LSCBS 388.61. Eight different agar media and six solid media were used in the experiment. Some morphological characteristics of mycelium on agar media were also evaluated. It was found that PDA was the best agar medium for mycelium growth of all tested strains. The strain LS302 was characterized by very high growth rate regardless of the examined agar medium. The tested strains presented changes in mycelium morphology on different agar media. The best mycelium growth was obtained on alder, larch and oak sawdust media, mainly in LSCBS 388.61, LS02 and LS286 strains.
The objective of the performed investigations was to compare mycelium growth of eighteen strains and two crossbred cultures of Lentinula edodes cultivated on agar media and on sawdust substrates from deciduous trees. On the basis of the performed experiments it is possible to conclude that the mycelium growth rate was a characteristic feature of the examined strains and crossbred cultures of L. edodes, although it depended on the type of the substrate on which it was growing.
The aim of the studies was to determine the effect of sawdust substrates and their enrichment with glucose on the mycelium growth and yield of three Hericium erinaceus (Bull. Fr.) Pers. strains. The subject of the studies was strains of H. erinaceus designated as ‘H1’, ‘D5’ and ‘D9’. Pine and beech sawdust supplemented with glucose in the amount of 1%, 2% and 3% were used as cultivated substrates. It was found that the tested strains differed in mycelium growth rate. The ‘D9’ strain was characterized by the fastest mycelium growth. The mycelium grew more rapidly on substrate with glucose addition, regardless from its amount, in comparison with substrate without glucose addition. The ‘H1’ and ‘D5’ strains gave the bigger yield than ‘D9’ one. The biggest yield was recorded on beech sawdust substrate with 3% addition of glucose.
Aroma in samples of two varieties of Agaricus bisporus, i.e. KORONA 7 and EUROMYCEL 12, was analysed in terms of contents of eight carbon atom compounds (1-octen-3-ol, 3-octanone, 3-octanol, 2-octen-1-ol). Carpophores were harvested in 4 flushes according to their cap sizes. Quantitatively the dominant compound in all samples was 1-octen-3-ol, found in the highest concentrations in the 1st flush of yielding. The analysis of variance for factorial experiments, performed for both cultivars in one cultivation cycle – 2001/2002 and for one variety – KORONA 7 in two cycles, showed the effect of mushroom variety, flush of yielding and carpophores size on contents of dry matter and aromatic volatiles. Carpophores of a smaller cap diameter were characterised by a higher dry matter content and usually also higher contents of aromatic volatiles. In contrast, the effect of flush of yielding was different: the highest content of dry matter was recorded in the 4th flush, while that of aromatic compounds in the 1st flush.
 A novel antibacterial protein with a molecular mass of 44 kDa has been isolated from dried fruiting bodies of the wild mushroom Clitocybe sinopica. Sodium dodecyl sulfate/polyacrylamide gel electrophoresis showed that the protein was composed of two subunits each with a molecular mass of 22 kDa. Its N-terminal amino-acid sequence, SVQATVNGDKML, has not been reported for other antimicrobial proteins. The purification protocol included ion exchange chromatography on DEAE-cellulose, CM-cellulose and Q-Sepharose, and gel filtration by fast protein liquid chromatography on Superdex 75. The antibacterial protein was adsorbed on all three ion exchangers. The antimicrobial activity profile of the protein against tested bacterial and fungal strains disclosed that it possessed potent antibacterial activity against Agrobacterium rhizogenes, A. tumefaciens, A. vitis, Xanthomonas oryzae and X. malvacearum with a minimum inhibitory concentration mostly below 0.6 μM. However, it had no antibacterial activity against Pseudomonas batatae, Erwinia herbicola, Escherichia coli, and Staphylococcus aureus, and no antifungal activity against Setosphaeria turcica, Fusarium oxysporum, Verticillium dahliae, Bipolaris maydis, and B. sativum. The antibacterial antivity against A. tumefaciens was stable after exposure to 20-60°C for 30 min and to pH 4-9 for 1 h.
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