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Early stages of trunk muscles development in a grass snake (Natrix natrix) L. (Reptilia, Colubridae)

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Lewandowski D., Posyniak E., Dubinska-Magiera M., Rupik W., Daczewska M.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2014

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tom 56

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nr 1

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Nano-nutrition of chicken embryos. Effect of gold and taurine nanoparticles on muscle development

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Zielinska M. K., Sawosz E., Chwalibog A., Ostaszewska T., Kamaszewski M., Grodzik M., Skomial J.

Journal of Animal and Feed Sciences

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2010

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tom 19

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nr 2

277-285

The objective of present experiment was to reveal influence of taurine, nanoparticles of gold or nanoparticles of gold conjugated with taurine on molecular and morphological status of muscle’s fibre proliferation activity at the end of embryogenesis. The expression of Proliferating Cell Nuclear Antigen (PCNA) was determined in embryonic chicken pectoral muscle using real time PCR and immunohistochemical method. Furthermore, we calculated the average number of nuclei within the muscle fibre fasciculus in cross sectional image. Taurine, supplemented in ovo to chicken embryo, enhanced organization of breast muscle by activating molecular mechanisms evaluated as an expression of PCNA. Nanoparticles of gold did not influence examined parameters.

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Expression of contractile protein-encoding genes TPM2 and TNNT3 during ontogenesis in pigs raised in Poland

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Rozycki M., Piorkowska K., Oczkowicz M.

Animal Science Papers and Reports

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2011

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tom 29

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nr 1

Troponin is a regulatory proteins complex composed of subunits TnC, TnI, and TnT encoded by separate genes – TNNT1, TNNT2 and TNNT3. It is a component of thin filaments (along with actin and tropomyosin), to which calcium binds to accomplish this regulation. The TPM2 gene encodes beta-tropomyosin. An imprinted QTL for muscle mass deposition has been detected near the TNNT3 gene and several significant linkages between TPM2 and QTLs on chromosome 1 have been identified. However, no significant correlation was found between polymorphism in both genes and economically important pig traits. The study aimed at analysing the level of expression of TNNT3 and TPM2 in the developing muscle (day 60-210 of life) and determining the expression differences among Polish Large White, Polish Landrace, Pulawska, Duroc and Pietrain pigs. Within the mentioned period the expression level of both genes in question did not change significantly. No developmental pattern characteristic for all breeds was revealed. In PL gilts a highly significant correlation was found among the level of expression in both white muscles (longissimus dorsi and semimembranosus) and the animals’ maternal origin. The above results suggest that an unknown polymorphism, probably located in regulatory part of the gene has an effect on the level of TNNT3 expression. Expression of the TPM2 did not change during ontogenesis and was not correlated with maternal origin. However, significant differences among gilts of different breeds were identified.

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Age-dependent changes in bovine skeletal muscle transcriptomic profile

80%

Sadkowski T., Jank M., Oprzadek J., Motyl T.

Journal of Physiology and Pharmacology. Supplement

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2006

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tom 57

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nr 7

95-110

The postnatal growth of muscle tissue occurs by hypertrophy comprising satellite cells proliferation, differentiation and protein turnover. The highest rate of skeletal muscle gains and protein synthesis in bulls occurs in the period between 180 and 360 days of postnatal life. However, genes which are responsible for quantitative and qualitative changes in skeletal muscle during this period are not identified up to date. The aim of our study was to compare the changes in transcriptomic profile of skeletal muscle (m. semitendinosus) in 12 Polish Black and White bulls between 6 and 12 month of life. For experimental purposes we used bovine cDNA microarray (the NBFGC EST collection) which contains 18,263 unique genes, derived from many different tissue types and various physiologically important states within these tissues. Our results revealed 53 genes which expression changed in the same manner depending on age of all examined pairs of animals. Thirty two of these genes showed at least 2-fold difference in expression between two analyzed age points. Age-dependent up-regulation was the most pronounced in the case of following genes: similar to MAD2L1 binding protein, similar to thymocyte potein thy28 isoform 1, similar to type I inositol-1,4,5-triphosphate 5-phosphatase, similar to nucleoside diphosphate kinase 6, proline rich 14, similar to transcription factor E2-alpha and phospholipase C gamma 1. The highest age-dependent decrease of the transcript was observed in the case of: similar to ubiquitin carboxy-terminal hydrolase L1, similar to latent TGF-beta binding protein 3 precursor, phospho-mannomutase 2, CD74 antigen, simlar to BCL6 co-represor-like 1, plateled/endothelial cell adhesion molecule (PECAM1), necdin, zygin, tight junction protein 3, ankyrin and apolipoprotein-L3. Although the role of the most of above genes and interactions between products of their expression is not clear at the moment, the significance of their response between 6 and 12 month of age may indicate their involvement in growth, development and metabolic changes in bovine skeletal muscle during the first year of postnatal life.

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The effect of cystatin B gene (CSTB) on productive traits in pigs

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Urbanski P., Pierzchala M., Terman A., Kamyczek M., Kapelanski W., Bocian M., Kawka M., Parada R., Roszczyk A., Rozycki M., Kuryl J.

Animal Science Papers and Reports

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2013

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tom 31

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nr 4

Cystatin B gene is a candidate gene for carcass and meat quality traits of pigs and belongs to the family 1 of cysteine proteinase inhibitors. The enzyme is a cathepsin inhibitor and the proteolytic cystatin/cathepsin system plays an important role in the growth and development of muscles.Investigations presented here covered 707 pigs from different genetic groups reared in Poland. The aim of this study was to characterise the polymorphism of the CSTB gene identified with restriction endonuclease: TaqI and PvuII, and to analyse the relation between the CSTB genotypes and carcass traits. All tested animals proved to be monomorphic at the CSTB/TaqI locus. All three possible genotypes were observed with regard to the second CSTB/PvuII locus. In Polish Large White and Polish Landrace pigs the highest frequency was reported for BB homozygotes. The association between CSTB and carcass traits was found only in Polish Landrace pigs for the meat content of carcass, meat content of valuable cuts and weight of the loin.

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