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Advanced methods of bacteriological identification in a clinical microbiology laboratory

100%

Zukowska M. E.

Journal of Pre-Clinical and Clinical Research

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2021

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tom 15

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nr 2

2

Rapid detection of Brachyspira hyodysenteriae and Lawsonia intracellularis in swine faecal and mucosal specimens by multiplex PCR

100%

Zmudzki J., Osek J., Stankevicius A., Pejsak Z.

Bulletin of the Veterinary Institute in Puławy

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2004

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tom 48

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nr 3

3

The prevalence of Ehrlichia canis, Anaplasma platys and Babesia spp. in dogs in Nueva Ecija, Philippines based on multiplex polymerase chain reaction (mPCR) assay

84%

Corales J. M. I., Viloria V. V., Venturina V. M., Mingala C. N.

Annals of Parasitology

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2014

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tom 60

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nr 4

The aim of the study was to determine the prevalence of Ehrlichia canis, Anaplasma platys and Babesia spp. in dogs. It describes the practice of veterinarians in detecting tick-borne diseases in Nueva Ecija, Philippines. Seventy blood samples were collected and were subjected to multiplex PCR for the detection of E. canis, Babesia spp. and A. platys. The prevalence of babesiosis is the highest in Cabanatuan City (2/10), while a 10% prevalence (1/10) was observed in Science City of Muñoz, Talavera and Sta. Rosa. E. canis were only detected in Cabanatuan City. However, no anaplasmosis was detected in any area. The prevalence of babesiosis and ehrlichiosis in Nueva Ecija is 7.14% (5/70) and 2.85% (2/70) respectively. In addition, 70% (7/10) of the Nueva Ecija veterinary practitioners encountered cases of suspected ehrlichiosis in their practice. The diagnosis of ehrlichiosis is based primarily on presented clinical signs and complete blood counts, which include a platelet count. Of the 10 respondents, half utilized test kits while 90% interpreted blood samples. Meanwhile, only 60% of the respondents used an ELISA test kit for ehrlichiosis. For some practitioners, the main reason for not utilizing a kit is the high cost. None of the respondents had previously attended cases of suspected anaplasmosis. Only one respondent diagnosed a case of babesiosis by blood smear microscopy.

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End point multiplex PCR for diagnosis of haemoprotozoan diseases in cattle

84%

Charaya G., Rakha N. K., Kumar A., Maan S., Goel P.

Acta Parasitologica

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2021

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tom 66

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nr 1

5

A PCR method targeting internal transcribed spacers: the simultaneous detection of Babesia bigemina and Babesia bovis in cattle

84%

Liu J., Guan G., Liu A., Li Y., Yin H., Luo J.

Acta Parasitologica

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2014

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tom 59

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nr 1

In this study, two pairs of oligonucleotide primers were designed according to the nucleotide sequence of the internal transcribed spacers (ITSs) of Babesia bigemina and B. bovis isolates from China. The primers were used in a multiplex PCR to detect parasite DNA in blood samples from cattle. There was no cross reactions with B. ovata, B. major, B. sp. Kashi, Theileria annulata, T. sergenti, T. sinensis or normal bovine DNA. The sensitivity of multiplex PCR assay was 1 pg and 10 pg DNA for B. bigemina and B. bovis, respectively. A total of 260 field blood samples collected from cattle in five provinces of China were analyzed by multiplex PCR and light microscopy. PCR testing revealed that 7.3% (19/260) and 5.8% (15/260) of cattle were positive for B. bigemina and B. bovis and 1.2% (3/260) of cattle were co-infected with B. bigemina and B. bovis. Using light microscopy, 2.3% (6/260) and 1.5% (4/260) of cattle were infected by B. bigemina and B. bovis, respectively, and no co-infection was found. The results showed that the multiplex PCR developed in the present study could be an alternative diagnostic tool for the detection of B. bovis and B. bigemina infection in cattle.

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Application of a multiplex PCR with specific PCR primers for the detection of the genus Paramecium and the Paramecium aurelia complex

84%

Haentzsch M., Bernhard D., Berendonk T. U., Przybos E., Schlegel M.

Acta Protozoologica

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2011

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tom 50

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nr 3

7

Some evidence for skewed mating type distribution in Iranian populations of Rhynchosporium commune, the cause of barley scald disease

84%

Arzanlou M., Karimi K., Mirabi F.

Journal of Plant Protection Research

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2016

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tom 56

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nr 3

Rhynchosporium commune (formerly known as Rhynchosporium secalis), the causal agent of scald disease on barley, is known to spread asexually by splash dispersed conidia. However, there are multiple lines of evidence for the possibility of a clandestine sexual cycle occurrence in this species including extensive genotypic diversity, equal distribution of mating type alleles across the world and expression of mating type genes. In the current study, the potential for the occurrence of a sexual cycle amongst the Iranian population of R. commune was assessed by analyzing distribution and frequency of the mating type alleles at both micro and macrospatial scales. A total of 95 single-conidial R. commune isolates were obtained from different barley fields in Kurdistan province. Previously designed primers were applied in a multiplex PCR assay to study distribution and frequency of the mating type alleles within and between populations. Totally, 67 isolates were determined as MAT1-1 and the remaining 28 isolates as MAT1-2 throughout the sampling counties. The results obtained at a macro-spatial scale revealed that unlike Kamyaran county (both MAT1-1 and MAT1-2 at an equal ratio), an unequal distribution of mating type genes was dominant among R. commune isolates in both Mariwan and Dehgolan counties. Our findings support a predominantly asexual reproduction for Mariwan and Dehgolan counties and the possibility of sexual stage occurrence in Kamyarna county.

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Application of multiplex PCR for the evaluation of the occurrence of ystA, ystB, ystC, and ymoA genes in Yersinia enterocolitica strains isolated from fattening pigs

67%

Bancerz-Kisiel A., Szczerba-Turek A., Platt-samoraj A., Szweda W.

Bulletin of the Veterinary Institute in Puławy

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2011

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tom 55

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nr 1

The purpose of the study was to evaluate the occurrence of genes directly connected with pathogenicity in Yersinia enterocolitica strains isolated from fattening pigs. Multiplex PCR, used for ystA, ystB, ystC, and ymoA gene detection, was optimised in order to determine the existence of the genes in one reaction. Material for the study consisted of 138 strains of Y. enterocolitica, which were preliminary examined by the bacteriological, sero- and biotyping methods, then bacterial DNA was isolated and multiplex PCR was performed. The presence of the products of length corresponding to the ystA and ymoA gene fragments was found in each of the examined strains. The ystB and ystC genes were not detected in any of the tested samples. The molecularly confirmed existence of Y. enterocolitica in fattening pigs indicates the carrier state and a possibility of shedding the microorganism into the environment, and shows that pigs are an important reservoir of the bacteria and a potential source of infection for humans.

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Identification of genes encoding classical staphylococcal enterotoxins in Staphylococcus aureus isolated from raw milk

67%

Korpysa-Dzirba W., Osek J.

Bulletin of the Veterinary Institute in Puławy

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2011

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tom 55

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nr 1

This study describes a multiplex PCR assay developed for the detection of Staphylococcus aureus enterotoxin types: SEA, SEB, SEC, SED, and SEE presented in the literature as classical. The method was then used to analyse the presence of genes encoding these enterotoxins in .S aureus strains isolated from raw milk. A total of 237 raw milk samples were used in the study and 77 (32.5%) of them were found to be contaminated with S. aureus. Among them, five isolates were harbouring the genes encoding staphylococcal enterotoxins - type C (three strains) and type A (two strains). These results show that raw milk can potentially be a source of staphylococcal food poisoning.

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First report of Trichinella pseudospiralis in Poland, in red foxes (Vulpes vulpes)

67%

Moskwa B., Gozdzik K., Bien J., Borecka A., Gawor J., Cabaj W.

Acta Parasitologica

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2013

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tom 58

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nr 2

Nematode worms of the genus Trichinella are one of the most widespread zoonotic pathogens. Natural transmission between hosts can only occur through the ingestion of infected meat. To date, two Trichinella species are known to be etiological agents of disease among domestic animals and wildlife in Poland: T. spiralis and T. britovi. In the last decades, since the administration of an oral vaccination against rabies, the red fox population in Poland has increased exponentially. The study area covers the Nowy Targ region: a mountainous area (585–1138 m above the sea) in southern Poland. Of 24 red foxes examined in the study, four were infected with Trichinella isolates: three were identified as T. britovi and one as T. pseudospiralis. The muscle of red foxes infected with T. britovi harboured 2.75, 3.11, 4.4 LPG and with T. pseudospiralis 0.36 LPG. Trichinella larvae were identified at species level by genomic and mitochondrial multiplex PCR, the products of which were sequenced for comparison with other sequences available in GenBank. The sequences obtained from the Polish T. pseudospiralis isolate, deposited in GenBank under the accession numbers JQ809660.1 and JQ809661.1, matched sequences already published in GenBank. Sequence comparison showed a 100% match with the large subunit ribosomal RNA gene of T. pseudospiralis isolate ISS 013, and a 96–95% match with those of T. pseudospiralis isolates ISS 141 and ISS 470. This is the first report of the identification of T. pseudospiralis larvae from red fox in Poland.

Ograniczanie wyników

Advanced methods of bacteriological identification in a clinical microbiology laboratory

Rapid detection of Brachyspira hyodysenteriae and Lawsonia intracellularis in swine faecal and mucosal specimens by multiplex PCR

The prevalence of Ehrlichia canis, Anaplasma platys and Babesia spp. in dogs in Nueva Ecija, Philippines based on multiplex polymerase chain reaction (mPCR) assay

End point multiplex PCR for diagnosis of haemoprotozoan diseases in cattle

A PCR method targeting internal transcribed spacers: the simultaneous detection of Babesia bigemina and Babesia bovis in cattle

Application of a multiplex PCR with specific PCR primers for the detection of the genus Paramecium and the Paramecium aurelia complex

Some evidence for skewed mating type distribution in Iranian populations of Rhynchosporium commune, the cause of barley scald disease

Application of multiplex PCR for the evaluation of the occurrence of ystA, ystB, ystC, and ymoA genes in Yersinia enterocolitica strains isolated from fattening pigs

Identification of genes encoding classical staphylococcal enterotoxins in Staphylococcus aureus isolated from raw milk

First report of Trichinella pseudospiralis in Poland, in red foxes (Vulpes vulpes)