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Comparison of multilocus variable-number tandem-repeat analysis with multilocus sequence typing and pulsed-field gel electrophoresis for Enterococcus faecalis

100%

Sadowy E., Sienko A., Hryniewicz W.

Polish Journal of Microbiology

2011

tom 60

nr 4

Enterococcus faecalis represents recently an important etiological agent of health care-associated infections (HAIs) and there is a need for evaluation and comparison of typing methods available for this microorganism. We tested multilocus VNTR (variable-number tandem repeats) analysis (MLVA) on a well-characterized collection of 153 clinical isolates of E. faecalis, corresponding to 52 multilocus sequence types and 67 pulsed-field gel electrophoresis (PFGE) profiles. MLVA showed high discriminatory power, discerning 111 different types (diversity index equal 98.9%). The concordance MLVA/MLST and MLVA/PFGE was 0.95 and 0.74, respectively. High discriminatory power of MLVA indicates its utility for local epidemiology such as outbreak investigation, and for differentiation of clones defined by other methods.

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Molecular identification of Trichoderma strains collected to develop plant growth-promoting and biocontrol agents

100%

Oskiera M., Szczech M., Bartoszewski G.

Journal of Horticultural Research

2015

tom 23

nr 1

Genotypes of Listeria monocytogenes strains isolated from 2000 to 2002 in Poland

80%

Paciorek J., Jacquet C., Salcedo C., Doumith M., Vazquez J. A., Martin P.

Polish Journal of Microbiology

2006

tom 55

nr 1

Pulsed field gel electrophoresis (PFGE), multiplex PCR and multilocus sequence typing (MLST) methods were used for genotyping study of seventy-three L. monocytogenes isolates collected in Poland between 2000 and 2002 from human, food, environment and a diseased goat. The multiplex PCR, which is an alternative method to classical serotyping, divided the isolates into four PCR groups, IIa (42.5%), IIb (27.4%), IIc (4.1%) and IVb (26%). The isolates displayed 33 distinct PFGE profiles. Twenty eight strains were further characterised by MLST based on sequence analyses of seven housekeeping genes. The combined sequence analyses revealed a total of 10 different allelic profiles from which 3 were not described earlier. It is intended that results obtained in this study will be the first data of a national database of L. monocytogenes genotypes in Poland.

Ograniczanie wyników

2 Polish Journal of Microbiology

1 Journal of Horticultural Research

1 Bartoszewski G.

1 Doumith M.

1 Hryniewicz W.

1 Jacquet C.

1 Martin P.

1 Oskiera M.

1 Paciorek J.

1 Sadowy E.

1 Salcedo C.

1 Sienko A.

1 Szczech M.

1 Vazquez J. A.

1 2015

1 2011

1 2006

Wyniki wyszukiwania

Comparison of multilocus variable-number tandem-repeat analysis with multilocus sequence typing and pulsed-field gel electrophoresis for Enterococcus faecalis

Molecular identification of Trichoderma strains collected to develop plant growth-promoting and biocontrol agents

Genotypes of Listeria monocytogenes strains isolated from 2000 to 2002 in Poland