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Coltsfoot leaves are a traditional raw material, rich in polysaccharides and phenolics. The variability of the contents of these main compounds was determined, using plant material originated from 22 natural populations of Tussilago farfara L. growing in various regions of Poland. In the years 2008–2009, plants from each investigated population were collected in the Garden of Medicinal Plants in Plewiska near Poznań (Poland). Coltsfoot leaves were harvested in the middle of June and July of 2010, and then dried at room temperature. In these raw material we quantified swelling index (describing mucilage content) and spectrophotometrically: the amounts of total polyphenols, polyphenols unadsorbed on hide powder (non-tannin phenolics) and tannins (expressed as pyrogallol equivalent) as well as the sum of hydroxycinnamic acid derivatives (expressed as rosmarinic acid) and flavonoids (expressed as quercetin). The demonstrated results show the relatively high and balanced contents of the basic active compounds, especially flavonoids (0.7–1.3%) and polysaccharides (swelling index: 8.0–14.5). In addition, it was found that flavonoids and mucilage in coltsfoot leaves fluctuate in only a small range (V=11–13%), regardless of overshadow and the harvest time of raw material.
One of the most popular and usable Aromatic plants are kinds of Basilica (Ocimum sp., Lamiaceae). This genius has different characteristics in behavior germination. Biodynamic agriculture is a new science in the research and especially the position of stars and planet relative to each other, position of moon around the earth and seasonal change and Solar and lunar eclipses. We must demonstrate this theory. In this experiment, we want to record the effect of based on two calendars (biodynamic and Astronomic) on three ecotypes of Basilica seed germination. The base of this experiment was a randomized block design with three replications. We used 50 seeds in Falcon tube (value=15ml) and recorded the weight and value of seeds in tube. Then we added 5ml of water in tube and shacked after moisturized and control and recorded the Mucilage value and another behavior of seed germination in lab condition. Results showed that different position of some planets such as moon and Mars and Jupiter had the highest effect, positive and significant effect on mucilage percent (p>95%), germination speed and length of root. Therefore, we could express the lunar position had the highest effect on root length of local type and the lowest effect on purple basil. Mucilage percentage of seed coat in green basil had the highest content relative to another ecotype and this content was significant (p>95%). Thus, the best suggestion for the produce of mucilage from the basil seeds, the best time is the first quadrature of the moon.
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