Wyniki wyszukiwania

1

Novobiocin sensitivity of Salmonella typhimurium dam and/or seqA mutants

100%

Chatti A., Aloui M., Tagourti J., Mihoub M., Landoulsi A.

Polish Journal of Microbiology

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2014

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tom 63

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nr 1

This study was carried out to determine the effects of novobiocin, a gyrase inhibitor, on the growth, survival, motility and whole cell proteins of S. Typhimurium dam and/or seqA strains. Our results showed that the dam and seqA/dam mutants are the most sensitive to novobiocin, compared to wild type and seqA strains. Surprisingly, the motility of seqA mutants increased after exposure to novobiocin only in stationary phase cells. All the other strains showed a significant decrease in their motility. The analysis of protein profiles of all strains demonstrated several modifications as manifested by the alteration of the expression levels of certain bands. Our work is therefore of great interest in understanding the effects of novobiocin on S. Typhimurium and the involvement of DNA methylation.

2

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Is concentration and motility of male gametes related to DNA damage measured by comet assay?

100%

Dobrzynska M. M., Tyrkiel E. J., Derezinska E., Ludwicki J. K.

Annals of Agricultural and Environmental Medicine

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2010

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tom 17

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nr 1

73-77

3

History traces of gastrointestinal motility in Poland

100%

Thor P. J., Laskiewicz J.

Journal of Physiology and Pharmacology. Supplement

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2003

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tom 54

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nr 3

145-154

The objectives of this chapter was to show how motility studies were developed and performed in Poland at the end of century to better understand pathophysiology and improve the clinicians ability to evaluate and treat patients with motility - related disorders. Some of the important historical points along the path to current understanding of the form and function of gastrointestinal motility are presented. Scarce information exists about other than Cracow and Wroc³aw motility centers in Poland in previous century. Lately sophisticated technology became available in Poland and more centers have begun to yield more effective strategies of treatment and enhanced understanding of the pathophysiologic mechanisms underlying GI motility disorders.

4

Murein-hydrolyzing activity of flagellin FlaA of Listeria monocytogenes

100%

Popowska M., Markiewicz Z.

Polish Journal of Microbiology

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2004

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tom 53

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nr 4

In this preliminary report we show that a 29 kDa surface protein of Listeria monocytogenes EGD removed from cells with 4 M LiCl has peptidoglycan (murein) hydrolyzing activity, as revealed by zymographic analysis using Bacillus subtilis murein and heat-killed Micrococcus luteus cells casted in the gel. Following two-dimensional electrophoresis, the protein was electroblotted to PVDF membrane and its identity (FlaA) was revealed by sequencing. Peptidoglycan hydrolysing activity of FlaA purified by FPLC on Mono-S Sepharose against labelled Escherichia coli murein was demonstrated.

5

Integrity of spermatozoa and their mitochondrial energetic status in relation to motility of spermatozoa

100%

Piasecka M., Kawiak J., Szych Z., Laszczynska M., Kozanecka A., Kolodziej B.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr 3

6

Cofilin-like protein influences the motility of Amoeba proteus

100%

Klopocka W., Wierzbicka K., Pomorski P., Krzeminski P., Wasik A.

Acta Protozoologica

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2006

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tom 45

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nr 4

7

In vitro study on the effect of aflatoxin on human spermatozoa

100%

Mathuria N., Verma R. J.

Acta Toxicologica

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2007

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tom 15

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nr 1

8

cGMP-dependent hydrolysis of cyclic nucleotides in peritoneal macrophages

100%

Wroblewska H., Kobialka M., Gorczyca W. A.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

9

The effect of time and temperature on motility of spermatozoa of common and grass carp

100%

Jezierska B., Witeska M.

Electronic Journal of Polish Agricultural Universities. Series Fisheries

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1999

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tom 02

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nr 2

The sperm collected from common and grass carp males was stored at 5oC, and activated with water of various temperatures (20, 26, and 30oC). Time of spermatozoa motility was measured. Motility decreased with time after milt collection. Common carp spermatozoa were active longer, up to 7080 s. In most series their activity was reduced after 24 hours. Spermatozoa of grass carp were active up to 3055 s, and their motility shortened already in 8 hours post collection. After 24 hours they were motile less than 10 s. The effect of temperature of activation was observed the spermatozoa were active for the longest time at 20oC. Spermatozoa motility time was also affected by temperature of storage. Even shortterm (15 min) keeping spermatozoa at 20oC shortened their motility time in both species, and after 2 hour storage common carp sperm motility was reduced by about 50%.

10

Effect of platelet-activating factor on motility, plasmalemma integrity, the process of capacitation and acrosome reaction of fresh and cryopreserved boar spermatozoa

84%

Kordan W., Lecewicz M., Tobolski J.

Polish Journal of Veterinary Sciences

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2009

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tom 12

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nr 2

175-181

The aim of the present study was to establish the effects of platelet-activating factor (PAF) on selected movement parameters, plasmalemma integrity, capacitation process and acrosome reaction in cryopreserved boar spermatozoa. A positive effect of PAF addition to cryopreserved semen on sperm motility was demonstrated, particularly with the application of phospholipid concentration of 1 x 10-6M-1 x 10-5M. A moderate induction of plasmalemma damage of cryopreserved spermatozoa was observed when PAF was used at a low concentration (1 x 10-8M-1 x 10-7M). The rate at which PAF induced the process of capacitation was inversely proportional to its concentration in the sample (the highest for the concentration of 1 x 10-8M, and the lowest at 1 x 10-5M). In turn, the strongest induction of acrosome reaction of spermatozoa was observed in samples with the addition of PAF at a concentration of 1 x 10-7M. The results obtained suggest that the application of PAF supplement to post-thawed boar semen can be used as a laboratory test of the ability of spermatozoa to induce the acrosome reaction.

11

The stage-specific function of gap junctions during tumourigenesis

84%

Czyz J.

Cellular and Molecular Biology Letters

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2008

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tom 13

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nr 1

92-102

Tumour development is a process resulting from the disturbance of various cellular functions including cell proliferation, adhesion and motility. While the role of these cell parameters in tumour promotion and progression has been widely recognized, the mechanisms that influence gap junctional coupling during tumorigenesis remain elusive. Neoplastic cells usually display decreased levels of connexin expression and/or gap junctional coupling. Thus, impaired intercellular communication via gap junctions may facilitate the release of a potentially neoplastic cell from the controlling regime of the surrounding tissue, leading to tumour promotion. However, recent data indicates that metastatic tumour cell lines are often characterized by relatively high levels of connexin expression and gap junctional coupling. This review outlines current knowledge on the role of connexins in tumorigenesis and the possible mechanisms of the interference of gap junctional coupling with the processes of tumour invasion and metastasis.

12

Effects of physical exercise on the motility of hands in patients operated because of Dupuytren's contracture in own material

84%

Deskur Z., Deskur A., Zawadzki M.

Central European Journal of Sport Sciences and Medicine

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2014

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tom 06

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nr 2

The purpose of this study was to evaluate the effect of physical exercise on limited locomotion in the hands of patients who underwent surgery to correct Dupuytren’s contracture. We studied the hands of 84 patients with Dupuytren’s contracture aged from 30 to 84 years. In all patients, the contracture was removed by performing a partial fasciectomy. Physical exercises were carried out a week prior to surgery and during the postoperative period. Patients were divided into group I, in which the exercise was carried out under the supervision of the authors of this study and group II, in which exercises were performed sporadically and without professional supervision. Measurements were performed on all patients one week before surgery (A), 1 week after surgery (B) and 6 weeks after (C). The range of movement of fingers was measured using a goniometer. The average total loss of finger extension was evaluated, taking due account of the extension loss in the MCP, PIP and DIP joints of all fingers of the treated hand. Rehabilitation treatment included active and passive exercises; in more severe cases the treatment of choice was massage and special equipment to help bear flexion contracture. Test results were statistically analyzed. In all patients, there was an increase in mobility of the fingers. Patients taking part in physical exercise had significantly greater range of finger movement.

13

The ethogram of Blepharisma japonicum (Protozoa, Ciliata)

84%

Ricci N., Tortorella V.

Acta Protozoologica

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1990

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tom 29

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nr 1

14

Exocrine gland morphogenesis: insights into the role of amphiregulin from development to disease

84%

Sisto M., Lorusso L., Ingravallo G., Lisi S.

Archivum Immunologiae et Therapiae Experimentalis

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2017

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tom 65

|

nr 6

15

Effects of solar radiation on motility, photomovements and pigmentation in two strains of the cyanobacterium, Phormidium uncinatum

84%

Hader D.-P., Hader M.

Acta Protozoologica

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1990

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tom 29

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nr 4

16

Effect of electromagnetic field on the sperm characteristics and histopathological status of testis in rats

84%

Aydin M., Turk G., Yuksel M., Cevik A., Apaydin A. M., Yilmaz S.

Medycyna Weterynaryjna

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2007

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tom 63

|

nr 02

178-183

The aim of the study was to investigate the effects of electromagnetic fields ELF-EMFs generated by 170 kV (50 Hz) high power lines on the epididymal sperm characteristics, biochemical parameters testosterone levels and histopathology of testis. The experimental group consisted of 28 adult male rats placed in an cottage 7.5 m far from transfer lines transferring 170 kV (50 Hz) energy. They were randomly divided into four groups of 7 rats’ each. Group 1, 2 and 3 were exposed continuously (24 hr) to ELF-EMF (48.21 ± 1.58 mG) for 1, 2 and 3 months, respectively. The rats of group three served as the control and were placed in laboratory conditions without a magnetic field. Insignificant (p > 0.05) decreases were determined among the groups in terms of reproductive organ weights, testes dimensions and epididymal sperm concentration and sperm motility, and an insignificant increment was observed in abnormal sperm rates in relation to the varying periods of exposure to the ELF-EMF. Although marked reductions (p < 0.001) were observed among the groups in relation to plasma and testis catalase activity, depending on exposure time, no significant differences were found in terms of glutathione and malondialdehyde levels. In the light of Johnsen’s testicular biopsy score the mean scores of groups 1, 2, 3 and control were determined as 9.24 ± 0.08, 8.02 ± 0.12, 6.98 ± 0.11 and 9.88 ± 0.07, respectively. Histopathology examinations of testis revealed a deceleration of spermatogenesis and degeneration of germ cell order in relation to exposure time.

17

Electrical activity of canine gallbladder

84%

Konturek J. W., Scott G. W., Kingma Y. J.

Journal of Physiology and Pharmacology

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1991

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tom 42

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nr 1

Our aims were to describe the myoelectrical activity in the single very thin layer of muscle of the canine gallbladder. The study was performed on 22 freshly removed canine gallbladders. Electrical activity was studied by the single sucrose-gap method and contractility of the tissue was measured simultaneously using a force transducer. The strips (15x1 mm) from different regions of gallbladder (fundus, corpus, neck) were cut in longitudinal, circular and oblique axes. The sucrose-gap apparatus together with connecting tubes, solutions and electrodes were kept at 37°C and the initial tension applied to the tissue was set to 1 g. In 82.7% of recordings, spontaneous myoelectrical activity consisted of regular rhythmic changes in membrane potential similar to slow waves recorded in intestinal tissue. The overall mean frequency was 11.4 ± 5.2 (mean ±SD) cycles per min: 11.1 ± 4.4 cycles per min in fundus, 11.9 ± 6.2 cycles per min in corpus and 10.8 ± 3.8 cycles per min in the neck of the gallbladder. In 84.2% of cases electrical activity correlated with mechanical activity and preceded it. No significant differences were seen between the electrical patterns in strips with different orientations or from the different regions of the gallbladder.

18

Role of cholecystokinin in postprandial and vagally stimulated duodenal and gallbladder motility in dogs

84%

Thor P., Laskiewicz J., Maczka M., Konturek S. J.

Journal of Physiology and Pharmacology

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1991

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tom 42

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nr 4

This study was designed to determine the role of cholecystokinin (CCK) in postprandial motility pattern of the duodenum and gallbladder (GB) in conscious dogs provided with chronic duodenal electrodes for recording of myoelectric activity and GB fistulas for measurement of intraluminal pressure and volume of GB and to calculate the GB motility index (MI) and GB emptying rate. During naturally occuring activity front (phase III MMC) in the duodenum there was significant increase in the MI of GB accompanied by about 20-30% reduction in the GB volume. These changes in duodenal and GB motility pattern could be duplicated by i. v. motilin. Feeding abolished the appearence of spontaneous activity front in the duodenum and greatly increased motility of GB while reducing its volume. Administration of CCK receptor antagonists in fed dogs failed to affect the motility changes induced by meal in the duodenum but abolished these of the GB. Vagal cholinergic stimulation with insulin, 2DG or urecholine caused similar effects to that induced by food i. e. increased duodenal spike activity, abolished phase III of the MMC, decreased GB volume and increased GB motility. Pretreatment with CCK antagonists did not affect significantly duodenal spike activity or GB motility but significantly increased the GB volume. Atropine 125 µg/kg) blocked almost completely spontaneous activity front in the duodenum and accompanying alterations in the motiliti and volume of GB. We conclude that CCK contributes to the MMC related alterations in the GB motor activity and is essential in cholinergic stimulation induced of the GB emtying but not in vagally induced duodenal and GB motility.

19

Helicobacter pylori infection in pathogenesis of gastroesophageal reflux disease

84%

Thor P. J., Blaut U.

Journal of Physiology and Pharmacology. Supplement

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2006

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tom 57

|

nr 3

81-90

Gastroesophageal reflux disease (GERD) refers to the very common and constantly increasing conditions where reflux of gastric contents into the esophagus leads to development of characteristic symptoms. The esophagus, LES and stomach can be envisioned as single functional unit controlled by neuro-hormonal factors. The abnormalities that contribute to GERD can start in any component of this unit, resulting particularly from disturbances in their control system. It is extremely important to identify factors and mechanisms leading to functional failure of this system so that causative therapy can be effectively applied. The key-role has been attributed to parasympathetic dysfunction, which may adversely affect motor activity of this area by increasing transient LES relaxation number and impairing LES pressure, esophageal acid clearance and motility of the proximal stomach. Recently, numerous investigations have been performed to elucidate the role of Helicobacter pylori (Hp) infection in GERD pathogenesis with the most concern given to its potency to increase gastric acid secretion. However, it appeared that this infection leads to much more complex changes in gastric mucosa including modification of afferent neural signals and specific gastric hormones release. Plasma ghrelin level is low in subjects infected and increases significantly after eradication. Since ghrelin, beside potency to increase gastric secretion has strong prokinetic action on LES functional unit, this phenomenon together with impaired vagal control may contribute to the Hp infection or eradication - related GERD development. Thus, ghrelin and vagal activity could be the missing links that partially explains relationship between GERD and Hp infection.

20

Effect of extender composition and storage temperatures on motility of preserved boar semen

84%

Fraser L., Lecewicz M., Krasicki R., Strzezek J.

Journal of Animal and Feed Sciences

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2002

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tom 11

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nr 4

Ograniczanie wyników

Novobiocin sensitivity of Salmonella typhimurium dam and/or seqA mutants

Is concentration and motility of male gametes related to DNA damage measured by comet assay?

History traces of gastrointestinal motility in Poland

Murein-hydrolyzing activity of flagellin FlaA of Listeria monocytogenes

Integrity of spermatozoa and their mitochondrial energetic status in relation to motility of spermatozoa