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The leaf epidermis of 36 taxa (species, subspecies, varieties and cultivars) of the genus Ficus (subgenera Ficus, Synoecia, Sycomorus, Pharmacosycea and Urostigma) was observed under LM and SEM. Characteristics of the epidermis are of particular taxonomic as well as phylogenetic interest because they differ conspicuously between species. The differences are especially prominent in the size and shape of epidermal cells, the shape of cell walls, peristomatal cuticular rim, the distribution and position of stomata, lithocysts, hydathodes, trichomes, and the types of wax, cuticle and trichomes. Results of this study provided data on new qualitative and quantitative traits of the leaf epidermis in the selected taxa, and confirmed the classification suggested by Berg (1989).
This paper presents the results of the study on morphological variability of leaves of a scattered tree species Sorbus torminalis (L.) Crantz) in Poland. The leaves from short- and long shoots were collected from 17 localities widespread within the range of the species in Poland. Leaves were measured according to 15 morphological traits. The biometric data were subjected to multivariate statistical analysis in attempt to define variability between local populations. Most of the leaf traits are significantly correlated and are characterised by moderate level of variation. The average among population component of variation was 32.82% and 27.46% for leaves on short- and long shoots, respectively. The differences between sampled populations are significant, but only a weak geographical pattern of this differentiation was detected. Clinal type of variation was ascertained in two traits. Leaf traits which discriminate best the studied populations are also indicated. It was proved that leaves on short shoots differ markedly in shape and size from those of long shoots. Leaves on long shoots are steadier, but morphological trait values are less correlated. The study also confirmed the occurrence of individuals with leaves characteristic for S. torminalis var. perincisa Borbas et Feck and S. torminalis f. mollis Beck in a few Polish populations.
The genus Plectranthus belongs to the family Lamiaceae is found in wild and cultivated condition. In the present work, the cultivars of Plectranthus forskohlii have been collected from Tamil Nadu, Kerala and Karnataka at different places. The accessions collected from Karnataka showed high tuber size, length and volume when compared to accessions collected from Tamil Nadu and Kerala. The most significant observation was made in Karnataka and Tamil Nadu cultivar accessions which were grown in the same soil in field condition have much branched root tuber with significant fresh weight as compared with accessions from Kerala. The Kerala accessions were observed with less diffused root tuber with low fresh weight. On the basis of the present research, it was concluded that the phenotypic variations present in leaf, stem and root characters might be due to some genetic heritable variation which may exist in the accessions collected from different locality, and this require further study with respect to their genotypic nature of Plectranthus forskohlii.
Morphological variability of parthenogenetic Cephalenchus hexalineatus and amphimictic Filenchus misellus was studied in populations of single female progenies reared on birch seedlings in laboratory for four months. In the females of both species the measurements and the indices related to the anterior body part (stylet length, pharynx length, position of excretory pore and hemizonid, MB, EP%L) and to the genital area (V, V’) were the least variable morphological characteristics. Ovary length, body width, anal body with, tail length, and indices a and c’ showed the greatest variability. The taxonomy of both species is discussed.
The morphological variation of Chara rudis A. Braun oospores was examined in a local population in a deep (43 m), hardwater (34 mg Ca dm–3) and mesotrophic (Ptot 0.095 mg P dm–3, SD 8 m) lake (NW Poland). Two variants of population density and three variants of depth were taken into account. Oospores are 712.8 ± 37.6 (600–817) μm long and 452.6 ± 34.2 (350–516) μm wide. They have 8–13 ridges. The outer membrane is slightly granulated. The isopolarity index (length/width × 100) is 158.4 ± 9.9 (140–190). The variation coefficient varies from 5% for oospore length to around 10% for fossa width. It was shown that the differences between oospores coming from individual depth and density variants are statistically significant. The biggest (733.21 ± 33.3 μm) oospores occurred on individuals growing at a depth of 3 m, while the smallest ones (673.3 ± 43.4 μm) at 5 m. The oospores from highly dense aggregations (> 50 individuals × 0.1 m–2) were much longer and wider than oospores from scattered (<10 individuals × 0.1 m–2) individuals. The observed intrapopulation variation of oospores can be regarded as a response to environmental changes along the depth gradient.
9-months tapeworms obtained from 3- 6-specimen populations of the 42nd generation of Hymenolepis diminuta WMS „strain" were compared with tapeworms obtained from quantitatively similar populations deriving from one-specimen infections of H. diminuta WMS il1. In both groups of tapeworms the majority of strobilas had unilateral position of genital pares (PGP), while the remaining ones were characterized by variable PGP. The latter appeared in 41.4% of H. diminuta WMS „strain" tapeworms and in only 24.6% of tapeworms coming from H. diminuta WMS il1. In tapeworms with unilateral as well as with variable PGP deriving from H. diminuta WMS il1, the average number of type 0p3a proglottids (all three testes on the aporal side) was significantly higher than in H. diminuta WMS „strain". It results most probably from suitable selection of mother specimens for successive generations of H. diminuta WMS il1. In both compared groups the growth of the number of PGP changes was correlated positively with the number of type 2p1a proglottids and negatively with the numer of type 1p2a and 0p3a proglottids.
Twelve morphometric variables of Cardaminopsis halleri individuals from four sites in upper silesia province were analyzed to establish the pattern of interpopulational relations. Two hundred plants were collected from two very polluted areas (near zinc-lead and zinc smelters) as well as from two other sites in unpolluted areas within the same bioclimatic region. stem size, dimensions of leaves and rosettes, as well as flower and seed numbers and in­dividual dry weights were studied using standard biometric methods. cluster analysis indicated overall differences among populations. Those four populations are different with respect to all variables. Discriminant function analysis confirmed seed number as the most important variable in the evaluation of interpopulational variability.
The analysis of size changes (length of placoliths, their width, length of central opening and its width) in elliptical reticulofenestrids from the NP25–NN5 zonal interval of the Central Paratethys allowed to dicriminate two size categories of placoliths: (i) small Reticulofenestra minuta (< 3.5 μm); (ii) Reticulofenestra haqii–pseudoumbilicus group (4–10.0 μm). The latter group appeared for the first time (FO, first occurrence) in the upper Egerian (size 4–7 μm) with the size of placoliths in this plexus increasing gradually. The FO of R. pseudoumbilicus > 8.0 μm has been established in the Zone NN2 while its FCO (first common occurrence) in the Zone NN5. This study shows that the FOs of size−defined morphotypes of the R. haqii–pseudoumbilicus group differ in the Central Paratethys and oceanic realm. Blooms of R. minuta at the Oligocene–Miocene boundary and in the Early Middle Miocene may be correlated with the incoming of warm water into the higher latitude Central Paratethys basins during connection with the Mediterranean Sea. Transgression favored the expansion of near−shore areas associated probably by some short−time oscillations of salinity. The FO of R. haqii–pseudoumbilicus group and the FO of R. pseudoumbilicus > 8.0 μm can be correlated with the opening of new pathways between the Mediterranean and the Central Paratethys. Gradual size changes in the R. haqii–pseudoumbilicus group probably reflect climatic changes: the decrease of coccolith size in the late Egerian may reflect cooling (Mi1 event) while the increase in coccolith size in the interval from the FO of Helicosphaera ampliaperta to the FO of Sphenolithus heteromorphus occurred due to warming. Two size categories of placoliths in the R. haqii–pseudoumbilicus group (3.5–6 μm and 6–8 μm) recorded in the interval from Zone NN1 to the lower part of the Zone NN2 may represent seasonal populations.
The most numerous limestone caves are in the smallest national park in Poland (the Ojców National Park). A total of 50 algal samples were collected from ten caves, each having different environmental factors such as light intensity, temperature and humidity. The morphological and ecological variability of cyanobacteria and algae were studied using fresh samples, cultures grown on agar plates, and documented with TEM, SEM and LM. Light microscopic observations showed that aerophytic cyanobacteria were the most important component of the cave′s photosynthetic microflora. Among cyanobacteria, the following genera were frequently encountered: Aphanocapsa, Chroococcus, Gloeocapsa, Leptolyngbya, and Synechocystis. Whereas the green algal genera, Apatococcus and Klebsormidium, often occurred with Chlorella, Muriella, Neocystis and the diatoms, Orthoseira and Pinnularia. Most of the algal species appeared to be cosmopolitan, ubiquitous, had simple nutrition requirements and wide ecological tolerance (they reproduced rapidly and were easily adaptable to new conditions). The cave′s microhabitats offered relatively stable microclimatic conditions and they seemed to be responsible for the observed distribution of aerophytic algae and cyanobacteria. The Shannon-Wiener index (H′) ranged between 4.9 and 3.9, and the Kruskal-Wallis test showed that these differences were statistically significant.
Fraxinus angustifolia Vahl is a species native in the north-western Balkans, where it has a divided range. Most of its range is located in flood-prone riparian sites in the Pannonian plain but the species is also found on humid sites along some rivers in the Submediterranean region. We analysed morphologic variation within and between five populations (14 trees/population) located in the Subpannonian and Submediterranean region of Slovenia. Thirty two characters were assessed on ca. 80 leaves from each tree which were divided depending on exposure to sunlight (sun and shade leaves), and 8 traits on each fruit (ca. 50 fruits/tree). A hierarchically designed experiment using analysis of variance confirmed the significant contribution of all the analysed hierarchical levels of variation to the total variation. The results show that the differences between the trees in a single population are the greatest factor of variability of leaves, followed by differences between populations and differences which are the result of variation in exposure to sunlight. Leaves in shade positions are typically larger than those in sunny positions, and they have broader and shorter-pointed leaflets; in this feature they are very similar to the leaves of F. excelsior. Stepwise discriminant analysis showed that the traits which refer to the leaflet number and length are the best differentiating traits between individual populations. The Submediterranean population Dragonja stands out with the smallest leaves and the population Lijak from the same region is the most similar to Dragonja for most morphological traits. In general, phenotypic differences between the Subpannonian and the Submediterranean populations of F. angustifolia are minor and indistinct. Based on the differences ascertained by the study, we can only confirm the presence of F. angustifolia subsp. oxycarpa (Bieb. ex Willd.) Franco et Rocha Alfonso in this part of its natural range.
The paper studies the morphological variability of the service tree populations in the continental and Mediterranean regions of Croatia. The inter- and intra-population variability, as well as eco-geographic population differentiation, was established on the basis of eight measured morphological characteristics of two types of leaflets (lateral and terminal) and two derived ratios, using descriptive and multivariate statistical methods. The conducted research established a high variability of morphological characteristics. In comparison with lateral leaflets, terminal leaflets showed a higher degree of variability for the majority of the studied variables. The differences between the trees within populations, as well as the differences between populations, were confirmed for all studied characteristics. The study also confirmed a trend of population variations according to the eco-geographic principle. Populations from climatically different and geographically distant habitats, in other words from the Mediterranean and the continental region, differed in the majority of studied characteristics. In addition to the established pattern of ecotypical variability between the continental and the Mediterranean regions, the clinal variability with regard to altitude and mean annual temperature was also confirmed. Small, although statistically significant clinal variability with regard to the longitude was recorded for two measured characteristics. The conducted study provided insights into the variability of the service tree populations in Croatia, which is a basis for further research that should be implemented in order to produce guidelines for the breeding and conservation of genetic resources of this rare and endangered noble hardwood species.
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