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Effect of non-enzymatic glycation on biological properties of morphiceptin

100%

Krawczuk S., Kostyra E., Kostyra H., Fraczek J.

Polish Journal of Food and Nutrition Sciences

2000

tom 09/50

nr 1

In the course of food proteins digestion, mid-molecular weight opioid peptides are generated. Their amine groups might undergo non-enzymatic glycation with glucose which is promoted by pH and temperature. The glycation conditions of morphiceptin (pH 7.8 and temp. 37°C) used in this experiment proved that non-enzymatic glycation of the peptides could take place in the digestive tract. The results obtained herein suggest that glycation decreases the contraction amplitude of isolated rabbit duodenum by 14.1% and changes the immunogenicity of morphiceptin (Tyr-Pro- Phe-Pro-NH2), probably due to a new epitope produced.

Effect of cerebral ventricles perfusion with morphiceptin and met-enkephalin on trigemino-hypoglossal reflex in rats

84%

Zubrzycka M., Fichna J., Janecka A.

Journal of Physiology and Pharmacology

2002

tom 53

nr 4,2

Opioids administered by intracerebroventricular injections produce analgesic responses in rats. The present study was undertaken to investigate the effects of a highly selective m-opioid receptor ligand morphiceptin on trigemino-hypoglossal reflex in rats. The analgesic effect of morphiceptin was compared with another opioid peptide, Met-enkephalin. With the experimental settings used in this study, we have demonstrated that both morphiceptin and Met-enkephalin show significant dose-dependent analgesic effects after i.c.v. administration in rats as assayed by trigemino-hypoglossal reflex test. The antinociceptive response to Met-enkephalin was short lasting and was observed 10 to 15 min after i.c.v. perfusion. Morphiceptin had a relatively longer duration of antinociceptive action, the effect was observed 20- 50 min after i.c.v. perfusion. Neither morphiceptin nor Met-enkephalin produced antinociception after peripheral injections. The results of the present study indicate that both tested peptides act at µ-opioid receptors situated in the central nervous system. They also suggest that µ-opioid receptors present in the central nervous system are an important element of the trigemino-hypoglossal reflex arc. For that reason selective m-opioid receptor ligands, like morphiceptin, inhibit the reflex more significantly.

Ograniczanie wyników

1 Journal of Physiology and Pharmacology

1 Polish Journal of Food and Nutrition Sciences

1 Fichna J.

1 Fraczek J.

1 Janecka A.

1 Kostyra E.

1 Kostyra H.

1 Krawczuk S.

1 Zubrzycka M.

1 2002

1 2000

Wyniki wyszukiwania

Effect of non-enzymatic glycation on biological properties of morphiceptin

Effect of cerebral ventricles perfusion with morphiceptin and met-enkephalin on trigemino-hypoglossal reflex in rats