The study aimed at evaluating the influence of soil contamination with nickel on a background of varied liming on cadmium, molybdenum, and sodium contents in red clover biomass. The four-year pot experiment was carried out using a completely randomized pattern in which the following factors were examined: I – soil contamination with nickel (0, 50, 100, 150 mg Ni kg⁻¹ soil); II – liming (no liming or liming according to 0.5, 1.0, and 1.5 Hhsoil). Red clover (Trifolium pratense L.) as a test plant of which the seeds were sown every year, with its biomass harvested 4 times per each vegetation season. The content of Na, Cd and Mo was determined by the ICP-EAS technique. Study results were statistically processed by applying variance analysis, while LSD(0.05)values were calculated according to the Tukey test. In order to find dependencies between examined features, linear correlation analysis was performed as well. The soil contamination due to nickel up to 100 mg kg⁻¹ soil caused a decrease of Cd content with an increase at the highest contamination level of 150 mg kg⁻¹ soil, while decrease of Na and Mo concentrations at all applied nickel rates. Liming made average Na and Cd contents lower and mean Mo level higher in red clover biomass.