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Immature zygotic embryo cultures of Arabidopsis. A model system for molecular studies on morphogenic pathways induced in vitro

100%

Kraut M., Wojcikowska B., Ledwon A., Gaj M. D.

Acta Biologica Cracoviensia. Series Botanica

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2011

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tom 53

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nr 2

To understand the molecular mechanism controlling in vitro plant morphogenesis, a culture system enabling induction of alternative morphogenic pathways (somatic embryogenesis, SE; shoot organogenesis, ORG) in a well defined population of somatic cells is needed. Arabidopsis is the most useful model plant for genomic studies, but a system in which SE or ORG can be induced alternatively in the same type of explant has not been proposed. Immature zygotic embryos (IZEs) of Arabidopsis provide the only explants with embryogenic potential, and have been recommended for studying mechanisms of SE induced in vitro. This study was aimed at defining culture conditions promoting induction of alternative morphogenic pathways: shoot ORG in IZE explants. The established protocol involves pretreatment of IZE explants with liquid auxin-rich callus induction (CIM) medium, followed by subculture on solid cytokinin-rich shoot induction medium (SIM). The method enables efficient shoot induction in Columbia (Col-0) and Wassilewskija (Ws), genotypes commonly used in molecular studies. During 3 weeks of culture up to 90% of Col-0 and 70% of Ws explants regenerated shoots via an indirect morphogenic pathway. We analyzed the qRT-PCR expression patterns of the LEC (LEC1, LEC2 and FUS3) genes, the key regulators of Arabidopsis embryogenesis, in the IZE explants induced to promote shoot ORG. The sharp decline of LEC expression on SIM medium confirmed that culture of Arabidopsis IZE explants enables experimental manipulation of the morphogenic response of somatic cells. A scheme illustrating various in vitro morphogenic responses of IZEs in relation to hormonal treatment is presented.

2

PCR-RFLP-based molecular studies on inter-population variability in the crucian carp (Carassius carassius L.)

84%

Kempter J., Panicz R., Makowska M., Metza M., Keszka S., Kielpinski M., Sadowski J.

Zeszyty Naukowe. Acta Biologica. Uniwersytet Szczeciński

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2011

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tom 18

Genetic variability of three sympatric crucian carp ( Carassius carassius ) populations from NW Poland was studied within a research project aimed at assessing the utility of those populations for stocking in inland waters. DNA samples were collected from 65 individuals. Restriction analysis was performed using 4 enzymes (HaeIII, HinfI, FspBI, TasI) of known restriction sites. The restriction pro files obtained were classified as belonging to a single haplotype (H-1). Selected DNA products were sequenced; the subsequent comparison made it possible to detect the presence of substi- tutions in the genome fragment analysed.

3

Molecular studies on plant S-nitrosoglutathione reductase

84%

Kubienova L., Kopecny D., Ticha T., Kopecna M., Briozzo P., Sedlarova M., Luhova L., Petrivalsky M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

|

nr 2

4

Characteristics of the grey seal ( Halichoerus grypus ) diet in the Vistula River mouth (Mewia Łacha Nature Reserve, Southern Baltic Sea), based on the osteological and molecular studies of scat samples

84%

Keszka S., Panicz R., Stepanowska K., Biernaczyk M., Wrzecionowski K., Zybala M.

Oceanologia

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2020

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tom 62

|

nr 3

5

Cultured endosperm versus endosperm in bread wheat caryopsis: biochemical, ultrastructural and molecular studies

84%

Popielarska-Konieczna M., Kleszcz I., Kwolek D., Marcinska I., Juhasz A., Makai S., Filek M., Sieprawska A., Kozieradzka-Kiszkurno M.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2016

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tom 58

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nr 1

6

Neuroprotective effects of onion and garlic root extracts against Alzheimer’s disease in rats: antimicrobial, histopathological, and molecular studies

84%

Hegazy E. M., Sabry A., Khalil W. K. B.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2022

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tom 103

|

nr 2

7

Molecular study of Prussian carp - an invasive species in the lakes of the Leszno Lakeland

84%

Panicz R., Keszka S., Rybczyk A., Zawal A.

Acta Biologica

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2016

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tom 23

8

Molecular studies on the variability of Polish semi-wild pears [Pyrus] using AFLP

84%

Dolatowski J., Nowosielski J., Podyma W., Szymanska M., Zych M.

Journal of Fruit and Ornamental Plant Research

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2004

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tom 12

|

nr Spec.ed.2

9

Molecular approach to the systematics of European Tortricini [Lepidoptera: Tortricidae]

67%

Razowski J., Tarcz S., Greczek-Stachura M.

Folia Biologica

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2010

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tom 58

|

nr 3-4

189-194

10

Loss of heterozygosity for Rb locus and pRb immunostaining in laryngeal cancer: a clinicopathologic, molecular and immunohistochemical study

67%

Pietruszewska W., Klatka J., Borzecki A., Rieske P.

Folia Histochemica et Cytobiologica

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2008

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tom 46

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nr 4

479-485

11

Molecular studies on intraspecific differentiation of Paramecium dodecaurelia, with description of new stands of the species [Protozoa, Ciliophora]

67%

Przybos E., Tarcz S., Greczek-Stachura M., Surmacz M., Potekhin A., Rautian M.

Folia Biologica

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2008

|

tom 56

|

nr 3-4

249-262

12

Length of restriction fragments is a factor determining DNA loss from metaphase chromosomes of Vicia faba

67%

Gernand D., Sakowicz T.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1992

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tom 40

|

nr 2

13

Aktualny stan wiedzy z zakresu genetyki ważniejszych roślin sadowniczych i zastosowanie jej w praktyce

59%

Bokszczanin K., Przybyla A. A.

Acta Scientiarum Polonorum. Biotechnologia

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2008

|

tom 07

|

nr 1

14

Metody analizy ekspresji genów w badaniach nad rzepakiem

59%

Olejnik A.

Rośliny Oleiste - Oilseed Crops

|

2013

|

tom 34

|

nr 1

W pracy przedstawiono przegląd metod analizy ekspresji genów z zastosowaniem technik: mikromacierzy DNA, seryjnej analizy ekspresji genów (SAGE), jak również odwrotnej transkrypcji – RT-PCR oraz qRT-PCR. Zaprezentowano możliwości wykorzystania poszczególnych technik wraz z przykładami ich praktycznego zastosowania w badaniach molekularnych rzepaku.

15

Fitoplazmy - nowe zagrozenie w produkcji roslin ozdobnych

59%

Kaminska M., Korbin M., Rudzinska-Langwald A.

Progress in Plant Protection

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1999

|

tom 39

|

nr 1

132-138

16

Badania cytogenetyczne i molekularne mieszancow miedzygatunkowych heksaploidalnego owsa Avena sativa L. x Avena sterilis L. oraz form wyjsciowych

51%

Paczos-Grzeda E.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

|

2003

|

tom 229

21-32

Ograniczanie wyników

Immature zygotic embryo cultures of Arabidopsis. A model system for molecular studies on morphogenic pathways induced in vitro

PCR-RFLP-based molecular studies on inter-population variability in the crucian carp (Carassius carassius L.)

Molecular studies on plant S-nitrosoglutathione reductase

Characteristics of the grey seal ( Halichoerus grypus ) diet in the Vistula River mouth (Mewia Łacha Nature Reserve, Southern Baltic Sea), based on the osteological and molecular studies of scat samples

Cultured endosperm versus endosperm in bread wheat caryopsis: biochemical, ultrastructural and molecular studies

Neuroprotective effects of onion and garlic root extracts against Alzheimer’s disease in rats: antimicrobial, histopathological, and molecular studies

Molecular study of Prussian carp - an invasive species in the lakes of the Leszno Lakeland

Molecular studies on the variability of Polish semi-wild pears [Pyrus] using AFLP

Molecular approach to the systematics of European Tortricini [Lepidoptera: Tortricidae]

Loss of heterozygosity for Rb locus and pRb immunostaining in laryngeal cancer: a clinicopathologic, molecular and immunohistochemical study

Molecular studies on intraspecific differentiation of Paramecium dodecaurelia, with description of new stands of the species [Protozoa, Ciliophora]

Length of restriction fragments is a factor determining DNA loss from metaphase chromosomes of Vicia faba

Aktualny stan wiedzy z zakresu genetyki ważniejszych roślin sadowniczych i zastosowanie jej w praktyce

Metody analizy ekspresji genów w badaniach nad rzepakiem

Fitoplazmy - nowe zagrozenie w produkcji roslin ozdobnych

Badania cytogenetyczne i molekularne mieszancow miedzygatunkowych heksaploidalnego owsa Avena sativa L. x Avena sterilis L. oraz form wyjsciowych