Wyniki wyszukiwania

1

Application of flow cytometer for molecular identification of Bacillus anthracis

100%

Michalski A. J., Bartoszcze M. A., Zabrocka L. B., Szymajda U.

Żywienie Człowieka i Metabolizm

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2007

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tom 34

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nr 3-4

Flow cytometry is a method of identification biological agents that has various applications. It has been applied for identifying many types of antigens in various materials, including environmental samples. Recently it has been noticed that this method could be used for molecular detection of biological agents. The purpose of this work was to apply flow cytometry with nested-PCR for the molecular identification of B. anthracis. Paramagnetic streptavidin-coated beads were used to capture the resulting fluorophore-labeled sequences. The results show that flow cytometry can be successfully used to detect specific fluorescein- dUTP and a biotin marked sequences.

2

Fungal molecular diagnostics: a mini review

100%

Atkins S. D., Clark I. M.

Journal of Applied Genetics

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2004

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tom 45

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nr 1

3

Detection and molecular identification of Cryptosporidium species among children with malignancies

86%

Ibrahim H. S., Shehab A. Y., Allam A. F., Mohamed M. A., Farag H. F., Tolba M. M.

Acta Parasitologica

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2021

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tom 66

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nr 2

4

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The importance of molecular identification methods of mosquitoes in epidemiology of vector-borne diseases

86%

Rydzanicz K., Lonc E., Becker N.

Annals of Parasitology

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2016

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tom 62

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nr Suppl.

5

Molecular identification, transcriptome sequencing and functional annotation of Pulex irritans

86%

Hu L., Zhao Y., Yang Y., Zhang W., Guo H., Niu D.

Acta Parasitologica

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2021

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tom 66

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nr 2

6

Molecular identification of polyhydroxyalkanoates-producing bacteria isolated from enriched microbial community

86%

Ciesielski S., Pokoj T., Mozejko J., Klimiuk E.

Polish Journal of Microbiology

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2013

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tom 62

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nr 1

Polyhydroxyalkanoates (PHAs) are especially interesting because of their similar properties to synthetic plastics and their potential use as biodegradable polymers. Many strategies have been employed to effectively and economically produce PHAs, among them a production process based on mixed microbial populations, enriched from activated sludge could be one of the alternative technologies. Defining the bacterial species creating these anonymous populations is crucial for the improvement of cultivation strategy. Moreover, enriched bacterial populations could be a promising source for microbes, useful in many biotechnological projects. The main object of this study was to characterize the microorganisms creating the microbial consortium cultured towards PHAs production. After cultivation, bacteria were identified using the 16S rRNA gene sequencing approach. The presence of genes engaged in PHAs synthesis was detected using PCR. The performed analysis revealed that among eleven isolated bacterial strains, four possessed the ability of polyhydroxybutyrate synthesis.

7

Isolation of thermotolerant pathogenic Naegleria australiensis from diverse water resources including household drinking water in Pakistan

86%

Tanveer T., Hameed A., Bin-Dukhyil A.-A. A., Alaidarous M., Matin A.

Acta Protozoologica

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2017

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tom 56

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nr 4

8

Neospora caninum natural infection in Tunisian rams: serological study and molecular identification of infection in semen

86%

Amdouni Y., Rouatbi M., Lassoued N., Rekik M., Gharbi M.

Acta Parasitologica

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2019

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tom 64

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nr 4

9

Morphological and molecular identification of a novel species, Ceratomyxa siganicola n. sp. (Myxozoa: Ceratomyxidae) from Siganus fuscescens, in East China Sea

86%

Zhang D., Zhao Y., Yang S., Yang C.

Acta Parasitologica

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2019

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tom 64

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nr 3

10

Molecular identification of Paramecium bursaria syngens and studies on geographic distribution using mitochondrial cytochrome C oxidase subunit I (COI)

86%

Zagata P., Greczek-Stachura M., Tarcz S., Rautian M.

Folia Biologica

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2015

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tom 63

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nr 1

11

Alveolar echinococcosis in a dog; analysis of clinical and histological findings and molecular identification of Echinococcus multilocularis

86%

Antolova D., Vichova B., Jarosova J., Gal V., Bajuzik B.

Acta Parasitologica

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2018

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tom 63

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nr 3

12

Molecular identification of blast resistance genes in rice genotypes using gene-specific markers

86%

Al-Daej M. I., Ismail M., Rezk A. A., El-Malky M. M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2019

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tom 100

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nr 3

13

Molecular identification and phylogenetic analysis of Heterakis dispar isolated from geese

86%

Bobrek K., Hildebrand J., Urbanowicz J., Gawel A.

Acta Parasitologica

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2019

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tom 64

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nr 4

14

Molecular identification of Echinococcus granulosus from wild European beaver, Castor fiber [L.] from North-Eastern Poland

86%

Tkach V. V., Swiderski Z., Drozdz J., Demiaszkiewicz A. W.

Acta Parasitologica

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2002

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tom 47

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nr 2

Partial sequence of mitochondrial ND1 gene was obtained from a hepatic cyst found in European beaver in the North-Eastern Poland. The sequence was identical to the previously published sequences of the G7 (pig) strain of Echinococcus granulosus. This is the first finding of the E. granulosus from European beaver.

15

Molecular evidence indicating that Przhevalskiana silenus, P.aegagri and P.crossii [Diptera, Oestridae] are one species

86%

Otranto D., Traversa D.

Acta Parasitologica

|

2004

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tom 49

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nr 2

Goat warble fly infestation is a subcutaneous myiasis present in many European and Asian countries. The causative agents of this myiasis have been assumed to be Przhevalskiana silenus (Brauer, 1858), P. aegagri (Brauer, 1863) and P. crossii (Patton, 1922) (Diptera, Oestridae). On the basis of morphological and biochemical data, some authors have considered P. silenus, P. aegagri and P. crossii as a single species. To clarify this taxonomie issue, the most variable regions of the mitochondrial gene encoding for the subunit I of cytochrome oxidase (COI) and of the 28S rRNA gene, were characterized molecularly for larvae morphologically identified as P. silenus, P. aegagri and P. crossii. The high genetic homology among the sequences of these putative species (≥ 99.71% for the COI gene and 100% for the 28S gene), indicates that P. aegagri and P. crossii are morphotypes of the single species P. silenus.

16

Molecular identification of Thelandros scleratus and Thelastoma icemi (Nematoda: Oxyruida) using mitochondrial cox 1 sequences

72%

Chaudhary A., Kansal G., Singh N., Shobhna K., Verma M., Singh H. S.

Acta Parasitologica

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2017

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tom 62

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nr 2

17

Application of DNA markers against illegal logging as a new tool for the Forest Guard Service

72%

Nowakowska J. A.

Folia Forestalia Polonica. Series A . Forestry

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2011

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tom 53

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nr 2

DNA markers are currently the most precise tool for forest tree species identification and can be used for comparative analyses of plant material. Molecular diagnosis of evidence and reference material is based on comparing the structure of DNA markers duplicated in the PCR reaction and estimation of the DNA profiles obtained in studied wood samples. For this purpose, the microsatellite DNA markers are the most suitable tool because of their high polymorphism and accurate detection of structural changes in the genome. The analysis of tree stump DNA profiles let avoid timely collection of data such as tree age, diameter, height and thickness, although such a piece of information may advantageous in wood identification process. For each examined tree species, i.e. Pinus sylvestris L., Picea abies (L.) Karst., Quercus robur L. and Q. petraea (Matt.) Liebl., Fagus sylvatica L., Betula pendula L., and Alnus glutinosa L., wood identification was possible via the DNA profiles established on a basis of minimum 4 microsatellite nuclear DNA loci, and at least one cytoplasmatic (mitochondrial or chloroplast) DNA marker. Determination of the DNA profiles provided fast and reliable comparison of genetic similarity between material of evidence (wood, needles, leaves, seeds) and material of reference (tree stumps) in the forest. This was done with high probability (approximately 98– 99%).

18

Simple verification of in vitro - grown clones of the genus Drosera L. using its molecular markers

72%

Fratrikova M., Bauer M., Jopcik M., Libantova J.

Acta Scientiarum Polonorum. Hortorum Cultus

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2018

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tom 17

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nr 1

Drosera L. is a genus of carnivorous plants that comprises approximately 250 species, although this number is probably not complete. Some of these taxa exhibit only small differences in morphological traits that can be partly influenced if the taxa are propagated in vitro. Here, we focus on the verification of putative clones of Drosera spathulata Labill., Drosera rotundifolia L. and Drosera binata var. Dichotoma species cultivated in vitro using molecular markers covering the internal transcribed spacer (ITS) region of 45S ribosomal DNA (rDNA). Following the polymerase chain reaction (PCR) amplification of ~360-bp DNA fragments and sequencing, the sequences were aligned with corresponding sequences in the National Center for Biotechnology Information (NCBI) database. In addition, each of tested PCR amplicons had a specific restriction profile that predominantly enables the differentiation of D. rotundifolia and D. spathulata; the shape of the leaves does not have to be a clear morphologically distinguishable trait.

19

Molecular identification of Diphyllobothrium latum and a brief review of diphyllobothriosis in China

72%

Guo A.-J., Liu K., Gong W., Luo X.-N., Yan H.-B., Zhao S.-B., Hu S.-N., Jia W.-Z.

Acta Parasitologica

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2012

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tom 57

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nr 3

Two tapeworm specimens collected in northeast China in 2009 and 2011 were identified as Diphyllobothrium latum based on morphological criteria. Molecular methods were used to confirm their identity and analyze genetic variations compared with published data for this species. Species identity was confirmed by molecular characterization of the 18S rDNA partial sequence, complete sequences of internal transcribed spacers (ITSs) and 5.8S rDNA, and partial sequences of mitochondrial cytochrome c oxidase subunit 1 (cox1) and mitochondrial NADH dehydrogenase subunit 5 (nad5). PCR amplification and sequence analysis of 18S rDNA (1472 bp), ITS regions (1218 bp), cox1 (885 bp), and nad5 (1028 bp) revealed that these four sequences showed more than 99% identity to reference sequences for D. latum, confirming that this species is D. latum. To date, a total of 12 diphyllobothriosis cases have been documented in China. This study represents the first molecular characterization of D. latum in China, providing molecular evidence of human diphyllobothriosis in China.

20

Molecular identification larvae of Onchobothrium antarcticum (Cestoda: Tetraphyllidea) from marbled rockcod, Notothenia rossii, in Admiralty Bay (King George Island, Antarctica)

72%

Laskowski Z., Rocka A.

Acta Parasitologica

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2014

|

tom 59

|

nr 4

Antarctic bony fishes are infected with cestode larvae belonging to the order Tetraphyllidea (parasites as adults in chondrichthyans). Larvae of the Tetraphyllidea differ from each other in the morphology of their scoleces and represent five forms. There are larvae with bothridia subdivided into one, two and three loculi, bothridia sac-like in shape and bothridia undivided with hook-like projections. Only one species of the family Onchobothriidae, Onchobothrium antarcticum, has been described from Antarctica and larvae with trilocular bothridia were assigned to this cestode species. In this study, ten larvae obtained from Notothenia rossii and three adult specimens of Onchobothrium antarcticum isolated from Bathyraja eatonii were examined. A partial sequence of cytochrome c oxidase subunit 1 of three adult specimens and four larvae was identical. The remaining six larval sequences differed from the sequences obtained from adult cestodes. Partial sequences of lsrDNA of all analyzed larvae were identical. These results confirm the taxonomic affiliation of the larvae with trilocular bothridia parasitizing marbled rockcod in Antarctica as Onchobothrium antarcticum.

Ograniczanie wyników

Application of flow cytometer for molecular identification of Bacillus anthracis

Fungal molecular diagnostics: a mini review

Detection and molecular identification of Cryptosporidium species among children with malignancies

The importance of molecular identification methods of mosquitoes in epidemiology of vector-borne diseases

Molecular identification, transcriptome sequencing and functional annotation of Pulex irritans

Molecular identification of polyhydroxyalkanoates-producing bacteria isolated from enriched microbial community

Isolation of thermotolerant pathogenic Naegleria australiensis from diverse water resources including household drinking water in Pakistan