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Evaluation of the prevalence and molecular characterization of Pneumocystis jirovecii in patients with a variety of respiratory diseases

100%

Sokulska M., Kicia M., Piesiak P., Matos O., Lobo M. L., Kopacz Z., Wesolowska M., Hendrich A. B.

Annals of Parasitology

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2016

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tom 62

|

nr Suppl.

2

Genetic and molecular characterization of pR351 plasmid from enteropathogenic Escherichia coli strain

100%

Czarny A., Lagowska-Zlotorzycka M., Mulczyk M.

Archivum Immunologiae et Therapiae Experimentalis

|

1989

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tom 37

|

nr 1-2

3

Molecular characterization of a distinct ginger chemotype from Thua Thien Hue, Vietnam, and the application of PCR-based markers for identifying unknown ginger populations in the region using machine learning

100%

Phan N. T. T., Nguyen D. T., Tong T. V. B., Dang H. H., Nguyen A. H., Tran B. D. D., Phan T. V., Tran L. T., Hoang Q. T., Nguyen T. Q. D. N., Truong P. T. B.

Acta Agrobotanica

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2024

|

tom 77

4

First molecular characterization of Trichostrongylus colubriformis infection in rural patients from Chile

86%

Hidalgo A., Gacitua P., Melo A., Oberg C., Herrera C., Fonseca-Salamanca F.

Acta Parasitologica

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2020

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tom 65

|

nr 3

5

Molecular characterisation of the Mycobacterium bovis causing bovine tuberculosis outbreaks in Poland

86%

Krajewska-Wedzina M., Kozinska M., Radulski L., Lipiec M., Augustynowicz-Kopec E., Weiner M., Szulowski K.

Journal of Veterinary Research

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2020

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tom 64

|

nr 1

6

Genetic diversity in Vernonia amygdalina Delile accessions revealed by random amplified polymorphic DNAs (RAPDs)

86%

Aikpokpodion P., Abebe J., Igwe D.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2018

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tom 99

|

nr 2

7

Molecular characterization of the niaD and pyrG genes from Penicillium camemberti, and their use as transformation markers

86%

Navarrete K., Roa A., Vaca I., Espinosa Y., Navarro C., Chavez R.

Cellular and Molecular Biology Letters

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2009

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tom 14

|

nr 4

692-702

Genetic manipulation of the filamentous fungus Penicillium camemberti has been limited by a lack of suitable genetics tools for this fungus. In particular, there is no available homologous transformation system. In this study, the nitrate reductase (niaD) and orotidine-5′-monophosphate decarboxylase (pyrG) genes from Penicillium camemberti were characterized, and their suitability as metabolic molecular markers for transformation was evaluated. The genes were amplified using PCR-related techniques, and sequenced. The niaD gene is flanked by the nitrite reductase (niiA) gene in a divergent arrangement, being part of the putative nitrate assimilation cluster in P. camemberti. pyrG presents several polymorphisms compared with a previously sequenced pyrG gene from another P. camemberti strain, but almost all are silent mutations. Southern blot assays indicate that one copy of each gene is present in P. camemberti. Northern blot assays showed that the pyrG gene is expressed in minimal and rich media, and the niaD gene is expressed in nitrate, but not in reduced nitrogen sources. The functionality of the two genes as transformation markers was established by transforming A. nidulans pyrG- and niaD-deficient strains. Higher transformation efficiencies were obtained with a pyrG-containing plasmid. This is the first study yielding a molecular and functional characterization of P. camemberti genes that would be useful as molecular markers for transformation, opening the way for the future development of a non-antibiotic genetic transformation system for this fungus.

8

Morphological and molecular characterisation of Myxidium kudoi Meglitsch, 1937 from the blue catfish Ictalurus furcatus, Valenciennes in Oklahoma, USA

86%

Woodyard E. T., Rosser T. G., McAllister C. T.

Acta Parasitologica

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2020

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tom 65

|

nr 2

9

Molecular characterization of atrazine resistance in common ragweed [Ambrosia artemisiifolia L.]

86%

Cseh A., Cernak I., Taller J.

Journal of Applied Genetics

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2009

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tom 50

|

nr 4

321-327

Common ragweed (Ambrosia artemisiifolia L.) is the most frequent weed in the Carpathian Basin and is spreading fast in other parts of Europe. In recent years, besides the wild type, a mutant genotype resistant to atrazine herbicides has evolved and is now widespread in many areas. The present study demonstrates that the atrazine resistance of ragweed is maternally inherited, and is caused by a point mutation in the psbA chloroplast gene. The promoter 5'-untranslated region and the open reading frame regions of the gene were analysed, and a homology search was performed. Both the atrazine-resistant and susceptible types of cpDNA were present in atrazine-resistant plants, while the mixed presence of both genotypes in the same plant, known as heteroplasmy, was not unequivocally detectable in susceptible plants.

10

Molecular characterization and seroprevalence of Echinococcus granulosus in wild boars (Sus scrofa) in south-western Iran

86%

Sarkari B., Mansouri M., Khabisi S. A., Mowlavi G.

Annals of Parasitology

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2015

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tom 61

|

nr 4

This study presents the first molecular and serological evaluation of Echinococcus granulosus infections in wild boars in Iran. Twenty five wild boars were collected in south-western Iran, during authorized hunting program, from March to October 2013, necropsied and examined for E. granulosus infection. Furthermore, seroprevalence of cystic echinococcosis in hunted boars was evaluated by an ELISA system. A fertile hydatid cyst due to E. granulosus was detected in the lung of one of the animals. Genotype analysis of the isolate was determined by analyzing a mitochondrial gene, cytochrome C oxidase subunit 1 (co1). DNA was extracted from the cyst sample and polymerase chain reaction amplification and DNA sequencing of the specific region of the co1 gene was performed. Molecular evaluation confirmed the presence of a sheep strain, the G1 genotype, in the wild boar in south-western Iran. This is the first report of the presence of G1 genotype of E. granulosus in wild boar in Iran. Serological evaluation of hydatid cyst by antigen-B ELISA revealed E. granulosus antibodies in 5 (20%) of 25 wild boars. A statistically significant difference was observed between the prevalence of E. granulosus antibodies and gender while the difference between the seroprevalence of E. granulosus and age was insignificant. Findings of this study might have important implications for the prevention and control of cystic echinococcosis.

11

Molecular characterization and phylogenetic analysis of Taenia multiceps from China

86%

Tan L., Wang A. B., Zheng S. Q., Zhang X. L., Huang C. J., Liu W.

Acta Parasitologica

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2018

|

tom 63

|

nr 4

12

Molecular and morphological characterization of Cysticercus tenuicollis in red deer (Cervus elaphus) from Turkey

86%

Cengiz G., Tenekeci G. Y., Bilgen N.

Acta Parasitologica

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2019

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tom 64

|

nr 3

13

Prevalence and molecular characterization of Theileria equi and Babesia caballi in jereed horses in Erzurum, Turkey

86%

Guven E., Avcioglu H., Deniz A., Balkaya I., Abay U., Yavuz S., Akyuz M.

Acta Parasitologica

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2017

|

tom 62

|

nr 1

14

Molecular characterization, tissue distribution, and expression profiling of the CTSD gene during goose ovarian follicle development

86%

Zhu J., Hu S., Lu Y., Rong Y., Qing E., Li L., Wang J.

Folia Biologica

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2021

|

tom 69

|

nr 1

15

Morphological and molecular characterization of Paramphistomum epiclitum of small ruminants

86%

Jadav M. M., Kumar N., Das B., Solanki J. B.

Acta Parasitologica

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2018

|

tom 63

|

nr 3

16

First report of molecular characterization and sequence phylogenetic analysis of Linguatula serrata from India

86%

Sudan V., Shanker D., Jaiswal A. K.

Acta Parasitologica

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2018

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tom 63

|

nr 4

17

Molecular characterization of Blastocrithidia culicis L17 ribosomal protein

86%

Manzine L. R., Da Silva T. A., Thiemann O. H., Cicarelli R. M. B.

Acta Protozoologica

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2006

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tom 45

|

nr 4

18

First report on the molecular characterization of Diaphorocleidus armillatus Jogunoori et al. 2004 (Monogenea: Dactylogyridae) infecting the gills of introduced fish, Gymnocorymbus ternetzi in India

72%

Chaudhary A., Verma C., Singh H. S.

Acta Parasitologica

|

2016

|

tom 61

|

nr 3

19

Observations of apparent lorica variability in Salpingacantha (Ciliophora: Tintinnida) in the Northern Pacific and Arctic Oceans

72%

Dolan J. R., Yang E. J.

Acta Protozoologica

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2017

|

tom 56

|

nr 3

20

Molecular characterization of the first internal transcribed spacer of rDNA of Parabronema skrjabini for the first time in sheep

72%

Hasheminasab S. S.

Annals of Parasitology

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2015

|

tom 61

|

nr 4

Parabronema skrjabini is a spirurid nematode of the family Habronematidae that lives in the abomasum of ruminants such as sheep and goats. The purpose of this study was to investigate the molecular aspects of Parabronema skrjabini in sheep. The worms were collected from sheep in Sanandaj (west of Iran). The first internal transcribed spacer (ITS) of ribosomal DNA (rDNA) nucleotide fragments of Parabronema skrjabini were amplified by polymerase chain reaction (PCR) using two pairs of specific primers (Para-Ir-R and Para-Ir-F). ITS1 homology in the sequence of this study was 69% compared with the sequence data in GenBank. To our knowledge, this is the first study in the world exploring the genetic diversity of P. skrjabini in sheep based on ITS1.

Ograniczanie wyników

Evaluation of the prevalence and molecular characterization of Pneumocystis jirovecii in patients with a variety of respiratory diseases

Genetic and molecular characterization of pR351 plasmid from enteropathogenic Escherichia coli strain

Molecular characterization of a distinct ginger chemotype from Thua Thien Hue, Vietnam, and the application of PCR-based markers for identifying unknown ginger populations in the region using machine learning

First molecular characterization of Trichostrongylus colubriformis infection in rural patients from Chile