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Modified substrates of DNA polymerases and design of antivirals

100%

Krayevsky A. A., Alexandrova L., Dyatkina N. B., Kukhanova M. K., Shirokova E. A.

Acta Biochimica Polonica

1996

tom 43

nr 1

The results obtained in our laboratory on investigating of substrate properties of a large number of compounds towards different DNA polymerases have been summarized. On the basis of systematic analysis a directed synthesis of nucleotides with antiviral properties was performed.

Thermodynamic contribution of nucleoside modifications to yeast tRNAPhe anticodon stem loop analogs

100%

Agris P. F., Guenther R., Sochacka E., Newmann W., Czerwinska G., Liu G., Ye W., Malkiewicz A.

Acta Biochimica Polonica

1999

tom 46

nr 1

The determination of the structural and functional contributions of natural modified nucleosides to tRNA has been limited by lack of an approach that can systematically incorporate the modified units. We have produced a number of oligonucleotide analogs, of the anticodon of yeast tRNAPhe by, combining standard automated synthesis for the major nucleosides with specialty chemistries for the modified nucleosides. In this study, both naturally occurring and unnatural modified nucleotides were placed in native contexts. Each oligonucleotide was purified and the nucleoside composition determined to validate the chemistry. The RNAs were denatured and analyzed to determine the van't Hoff thermodynamic parameters. Here, we report the individual thermodynamic contributions for Cm, Gm, m1G, m5C, Ψ. In addition m5m6U, m1Ψ, and m3Ψ, were introduced to gain additional understanding of the physicochemical contribution of Ψ and m5C at an atomic level. These oligonucleotides demonstrate that modifications have measurable thermodynamic contributions and that loop modifications have global contributions.

Ograniczanie wyników

2 Acta Biochimica Polonica

1 Agris P. F.

1 Alexandrova L.

1 Czerwinska G.

1 Dyatkina N. B.

1 Guenther R.

1 Krayevsky A. A.

1 Kukhanova M. K.

1 Liu G.

1 Malkiewicz A.

1 Newmann W.

1 Shirokova E. A.

1 Sochacka E.

1 Ye W.

1 1999

1 1996

Wyniki wyszukiwania

Modified substrates of DNA polymerases and design of antivirals

Thermodynamic contribution of nucleoside modifications to yeast tRNAPhe anticodon stem loop analogs