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1

Optimisation of the time of milk sample decontamination with 0.75 percentage cethylpirydinium chloride upon identification of Mycobacterium avium subsp. paratuberculosis

100%
Wiszniewska A., Szteyn J., Fus M. M., Gomolka-Pawlicka M.
Polish Journal of Veterinary Sciences
|
2004
|
tom 07
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nr 2
2
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Investigation of the presence of Aspergillus flavus in mastitis milk with PCR

86%
Sukru K., Ugur P., Ugur A., Tugba Y. H.
Environment, Earth and Ecology
|
2019
|
tom 3
|
nr 1
3

Influence of region and lactation period on DDT and lindan content in mares' milk from Poland

72%
Pietrzak-Fiecko R., Borejszo Z., Zaluska O., Smoczynska K., Tomczynski R., Smoczynski S. S.
Bulletin of the Veterinary Institute in Puławy
|
2009
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tom 53
|
nr 1
83-87
Milk samples were collected from 2000 to 2001, between 7th to 180th d of lactation from mares differing in age and the number of lactations. The content of DDT and HCH was determined by gas chromatography with electron capture detection. It was confirmed that mare's milk was contaminated with various amounts of hazardous residues of DDT and its metabolites, probably depending on stud localisation.
4
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Molecular typing of Staphylococcus aureus based on PCR-RFLP of coa gene and RAPD analysis

72%
Karakulska J., Pobucewicz A., Nawrotek P., Muszynska M., Furowicz A. J., Czernomysy-Furowicz D.
Polish Journal of Veterinary Sciences
|
2011
|
tom 14
|
nr 2
The aim of this study was molecular identification of S. aureus strains isolated from mastitic milk samples and establishing the genetic relationship between strains isolated from cows belonging to the same herd. In all 43 isolated strains the gap gene (930 bp) was amplified, which enabled their affiliation to the Staphylococcus genus to be established. PCR-RFLP with AluI endonuclease of the gap gene as well as nuc (450 bp) and coa (1130 bp) gene amplification allowed precise S. aureus species identification. One hundred percent of the genetic relationship between strains was established via RAPD-PCR and coa-typing.
5

Biodiversity of Lactococcus lactis bacteriophages in Polish dairy environment

72%
Szczepanska A. K., Hejnowicz M. S., Kolakowski P., Bardowski J.
Acta Biochimica Polonica
|
2007
|
tom 54
|
nr 1
We present here the results of an exploration of the bacteriophage content of dairy wheys collected from milk plants localized in various regions of Poland. Thirty-three whey samples from 17 regions were analyzed and found to contain phages active against L. lactis strains. High phage titer in all whey samples suggested phage-induced lysis to be the main cause of fermentation failures. In total, over 220 isolated phages were examined for their restriction patterns, genome sizes, genetic groups of DNA homology, and host ranges. Based on DNA digestions the identified phages were classified into 34 distinct DNA restriction groups. Phage genome sizes were estimated at 14-35 kb. Multiplex PCR analysis established that the studied phages belong to two out of the three main lactococcal phage types - c2 and 936, while P335-type phages were not detected. Yet, analyses of bacterial starter strains revealed that the majority of them are lysogenic and carry prophages of P335-type in their chromosome. Phage geographical distribution and host range are additionally discussed.
6

Immunobiology of bovine mammary gland: apoptosis of somatic cells in milk during naturally occurring mastitis

72%
Cywinska A., Bas M., Karpiuk O., Krzyzowska M., Rzewuska M., Schollenberger A., Niemialtowski M.
Polish Journal of Veterinary Sciences
|
2006
|
tom 09
|
nr 1
Mastitis remains a major cause of economic losses in dairy herds. It is believed, that the enhancement of natural defense mechanisms in mammary gland may be helpful in the treatment of bovine mastitis. Our study was designed to assess the apoptosis of leukocytes isolated from bovine milk during subclinical staphylococcal mastitis. Milk samples were collected from cows naturally infected with one pathogen - Staphylococcus aureus and from animals with mastitis caused by several pathogens, including S. aureus. It has been determined that the rate of apoptosis was lower in mastitic milk, as compared with control samples, although varied significantly between groups. High percentage of apoptotic cells was detected in milk with high counts of pathogenic bacteria. In all groups the rate of apoptosis was dependent on the bacterial load, although various correlations were identified. Thus, it is postulated, that the rate of apoptosis of somatic cells in mastitic milk is related to the etiology of infection and is determined by the bacterial load.
7
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The presence of anti-Hypoderma antibodies in udder milk samples, and correlation with selected parameters of dairy performance

58%
Bednarko-Mlynarczyk E., Szteyn J., Bialobrzewski I., Wiszniewska-Laszczych A., Liedtke K.
Polish Journal of Veterinary Sciences
|
2012
|
tom 15
|
nr 3
Hypodermosis is responsible for significant economic losses and remains a current problem in animal husbandry. This study determined the extent of Hypoderma spp. invasion in north-eastern Poland in dairy herds. The extent of the invasion, determined on the basis of positive results of an ELISA assay (Bovine Hypodermosis Milk Screening of Institut Pourquier), varied in herds. Statistical analysis performed on the results of milk productivity and content of basic components revealed a decrease in the milk yield and changes in milk protein in animals revealing positive, doubtful and negative results. Statistically significant differences were absent as regards the content of milk fat or milk total solids. The milk yield and the content of particular milk components were determined using the AT4 method, as part of a control program carried out by the Polish Federation of Cattle Breeders and Dairy Farmers (PFHBiPM).
8
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Prevalence of tick-borne encephalitis virus [TBEV] in samples of raw milk taken randomly from cows, goats and sheep in Eastern Poland

58%
Cisak E., Wojcik-Fatla A., Zajac V., Sroka J., Buczek A., Dutkiewicz J.
Annals of Agricultural and Environmental Medicine
|
2010
|
tom 17
|
nr 2
283-286
9

The concentrations of beta-carotene, vitamin A and vitamin E in bovine milk in regard to the feeding season and the share of concentrate in the feed ration

58%
Strusinska D., Antoszkiewicz Z., Kaliniewicz J.
Roczniki Naukowe Polskiego Towarzystwa Zootechnicznego
|
2010
|
tom 06
|
nr 4
10

Comparison of dairy performance of Montbeliarde and Black-and-White cows housed in the same environmental conditions

58%
Golebiewski M., Brzozowski P.
Annals of Animal Science
|
2008
|
tom 08
|
nr 1
3-11
11

Antioxidative-related activities of lactoferrin and lactoperoxidase in milk from cows with different forms of mastitis

58%
Szczubial M., Kankofer M., Dabrowski R.
Bulletin of the Veterinary Institute in Puławy
|
2011
|
tom 55
|
nr 1
Milk samples were taken from cows with acute, subacute, chronic, and subclinical mastitis and from healthy cows. The mean activity of lactoferrin (LF) in milk from mastitic cows ranged from 8.9 ±3.0 to 12.1 ±6.9 mU/g protein and was significantly lower than that in milk from healthy cows (29.5 ±15.0 mU/g protein). In group of mastitic cows the highest LF activity was found in cows with chronic mastitis, and the lowest in those with subclinical mastitis. The lactoperoxidase activity in cows with clinical and subclinical mastitis was significantly higher in comparison with healthy cows (1.3 ±1.1 mU/g protein) ranging from 5.5 ±2.6 mU/g protein in subclinical mastitic cows to 8.4 ±5.0 mU/g protein in chronic mastitic cows. Lower LF activities in cows with mastitis than in healthy animals may lead to a decreased antioxidant defence system in mastitic cows.
12
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Levels of ochratoxin A in IgG against conidia of Penicillium verrucosum in blood samples from healthy farm workers

58%
Skaug M. A.
Annals of Agricultural and Environmental Medicine
|
2003
|
tom 10
|
nr 1
Ochratoxin A (OTA) is a mycotoxin frequently found in human blood and milk samples in the colder climatic zones. In addition to dietary intake, exposure may occur by inhalation of toxin containing fungal conidia. The purpose of this work was to investigate the level of OTA in blood samples from farm workers and non-farm working controls, and to examine if serum levels of OTA were related to inhalatory exposure to conidia of Penicillium verrucosum, the main OTA producer in temperate climates. Blood samples from 210 participants were analysed for the presence of OTA and IgG antibodies against P. verrucosum conidia. The concentration of OTA was determined by HPLC (DL 10 ng/l), and the IgG level was determined by ELISA. All serum samples contained OTA (mean 397 ng/l, range 21-5534 ng/l). The OTA level in serum was unrelated to farm working, gender, age, and IgG level. The mean IgG level was significantly higher among farm workers than controls. Farm working, or increased inhalatory exposure to P. verrucosum, was not related to higher OTA serum levels. Inhalatory exposure to OTA from farm working seems to be of minor importance compared to dietary intake.
13

Porównanie codziennego oznaczania zawartości tłuszczu z okresowym badaniem próbek konserwowanych w mleku surowym do skupu

51%
Bazydlo I., Pachucki T., Sokolowski T., Kirklewska A.
Acta Academiae Agriculturae ac Technicae Olstenensis. Oeconomica
|
1990
|
nr 25
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