Wyniki wyszukiwania

1

Zastosowanie odczynu ELISA do diagnostyki zakazen mykoplazmami u drobiu

100%

Tomczyk G.

Medycyna Weterynaryjna

|

1996

|

tom 52

|

nr 07

448-452

The objective of the studies was development and use of a customized ELISA kit to diagnose mycoplasmal infections in poultry. The frozen strain of S-6 M. gallisepticum, concentrated and ultra-sounds processed, served as the antigen. The best extinction (OD) was noted with the antigen concentration of 100 µg/ml, sera diluted at 1:100 and conjugate diluted at 1:250. It was best to preserve antigen coated plates overnight at 4°C. The ELISA was used to detect specific anti-M. gallisepticum antibodies both in chickens experimentally infected and in hens and turkeys naturally infected with this microorganism. Basing on the calculated value S/P it was possible to compare the sensitivity of the ELISA with an agglutination test (SPA) and a hemagglutination inhibition test (HI). The value of S/P for positive sera was more than 0.5, while for negative sera, below 0.5. The value of S/P in the ELISA for sera of chickens experimentally infected with high positive titres in HI (320 and 640) was more than 2.5, and for sera of HI titre 80 these values of S/P ranged from 1.0 to 1.5. The investigation of naturally infected birds showed that S/P in the ELISA test for sera of full agglutinating properties (3+) was more than 2.0, and for individual sera it was up to 3.5. However, weakly positive sera in the SPAS have a negative or slightly positive S/P value (from 0.5 to 1.0). The ELISA test used to detect antibodies specific to M. gallisepticum proved to be more sensitive than the SPA and HI test.

2

Antybiotykooporność i mechanizmy jej powstawania w przypadku zakażeń Mycoplasma bovis u bydła

86%

Szacawa E., Horecka A., Bednarek D., Niemczuk K.

Medycyna Weterynaryjna

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2014

|

tom 70

|

nr 07

Mycoplasma bovis is the smallest known bacterium that does not have a cell wall. It is therefore resistant to some antibiotics that inhibit the synthesis of the cell structure. Little is known about the mechanisms of antibiotic resistance in M. bovis, since it has no plasmids and there are insufficient data about the role of the biofilm formation by these bacteria. Previous studies have shown that the development of antibiotic resistance is due to gene mutations. Antibiotics generally considered as effective against M. bovis infection are macrolides, fluoroquinolones, tetracyclines, lincosamides, aminoglycosides, and chloramphenicol. Several recent studies, however, indicate that the efficacy of tetracyclines, macrolides, and lincosamides has diminished. Increased resistance to erythromycin, spectinomycin, and tilmicosin, antibiotics commonly used in the treatment of M. bovis infections, has also been reported. Among field strains of M. bovis no resistance or rare resistance has only been observed for enrofloxacin, florfenicol, tylosin, and tulathromycin. Considering the rapidly growing antibiotic resistance of the isolated strains of M. bovis, it is necessary to search for alternative compounds that could effectively inhibit these bacteria.

3

Czy organizmy prokariotyczne maja cytoszkielet?

86%

Skowronek K.

Kosmos

|

2001

|

tom 50

|

nr 3

201-211

4

Występowanie zakażeń mikoplazmami u gęsiorów i kaczorów piżmowych używanych jako dawcy nasienia w inseminacji

72%

Tomczyk G., Minta Z., Lukaszewicz E.

Zeszyty Naukowe. Przegląd Hodowlany

|

1997

|

tom 31

5

Zakazenia kur Mycoplasma gallisepticum/synoviae w swietle badan serologicznych

72%

Wieliczko A., Mazurkiewicz M., Wisniewska J.

Medycyna Weterynaryjna

|

2000

|

tom 56

|

nr 04

240-244

Mycoplasmosis avium is a highly infectious disease, which has been diagnosed mainly on commercial poultry farms. It usually occurs in meat-type flocks of hens, turkeys, and also in ducks and geese. The purpose of the study was to define the level of dissemination of infections caused by mycoplasmas in flocks of reproduction meat-type hens and broilers. The experiment covered 142 reproduction meat-type flocks (1 day - 65 week-old birds) and 136 broiler flocks (1-7 weeks old birds). The materials used in the studies were blood samples collected from the farm birds in 1998-1999 (until August). Blood samples were collected once in layers and in 109 broiler flocks, and twice in the other 27 broiler flocks, i.e. in one-day chicks and in the 6,h week of rearing. Moreover, additional 15 broiler farms were included in the serological monitoring (tests performed on 1st day, 2nd - 3rd and 6,h - 7th week of rearing). The level of M. gallisepticum and M. synoviae antibodies in serum was determined by ELISA (Idexx kit with MG/MS antigen) and additionally by RPA test (MG and MS antigen by Intervet) on 15 farms. The presence of specific Mycoplasma antibodies was found in 54.9% of the layer flocks monitored. However, the highest percentage of serologically positive flocks was recorded in the laying period (65.2%) and the lowest in one-day old chickens (30.8%). Infection with Mycoplasmosis in broilers reached 44.1%, and in these cases the occurrence of MG/MS antibodies was observed more often in one-day old chicks (55.1%) than in 6- -7 week old chickens (32.1%). The flocks of broilers demonstrated more infections with M. synoviae than with M. gallisepticum (25.9% and 7.4%, respectively in the 6th week).

6

Szczepionka przeciwko bronchopneumonii cielat wywolanej przez Mycoplasma bovis

72%

Pilaszek J., Kondracki M.

Życie Weterynaryjne

|

1994

|

tom 69

|

nr 07

275-278

7

Profilaktyka zakazen Mycoplasma gallisepticum

72%

Olczyk B.

Hodowca Drobiu

|

2000

|

nr 08

25-27

8

Zwalczanie zespolu oddechowego swin

72%

Pejsak Z.

Nowoczesne Żywienie Zwierząt

|

2002

|

nr 5

15-17

9

Spostrzezenia nad mozliwosciami klinicznego zastosowania nowej kombinacji antybiotykow - kolistyny ze spiramycyna

72%

Rolinski Z., Duda M., Furmaga R., Kowalski C.

Medycyna Weterynaryjna

|

1992

|

tom 48

|

nr 03

104-107

There have been done a wide review on the physico-chemical properties of colistin and its antibacterial activity. The antibiotic of peptide nature is very effective against the majority of gram-negative bacterial cells. Colistin is not absorbed from the alimentary tract and therefore it should be given in case of intestine infections caused by E. coli or Salmonella spp instead of sulfamides or tetracyclines (usually not effective). On the other hand chloramphenicol is not allowed to use because of its side effects. Since spiramycin is effective against gram-positive bacteriac and mycoplasms therefore given together with colistin has a wide spectrum of activity. Besides, spiramycin inhibits the process of intestine colonisation by E. coli and colistin kills these bacteriae. According to the data of literature the effectiveness of the two antibiotics administered together has been confirmed in mycoplasmatic infections of the respiratory tract of chickens complicated by pathogenic strains of E. coli. In the studies performed by the authors there was found a high effectiveness of these antibiotics combination in a perihatching period infections (Belcospira orale — Rhone Merieux), in colibacteriosis of piglets and in secondary infections of influenza in horses (Belcospira injectable — Rhone Merieux).

10

Wlasciwosci biologiczne Mycoplasma hyopneumoniae, czynnika etiologicznego mykoplazmowego zapalenia pluc

72%

Kolodziejczyk P., Pejsak Z.

Medycyna Weterynaryjna

|

2004

|

tom 60

|

nr 04

341-344

11

Szczepionka przeciwko mykoplazmozie kur

72%

Pilaszek J., Truszczynski M., Wijaszka T., Musialik M.

Medycyna Weterynaryjna

|

1994

|

tom 50

|

nr 12

600-602

Attempts have been made to prepare a vaccine against poultry toxoplasmosis. Mycoplasma gallisepticum antigen mixed with an oil adiuvant (Emulsigen) was used. The vaccine passed innocuity and potency tests carried out under laboratory conditions positively. It has been found that intramuscular administration of 0.3 ml of the vaccine to 3-5 week-old chickens produced the best immunological response. Totally, over 12 000 chickens have been vaccinated in field conditions with good results determined on the basis of epidemiological observations.

12

Sprawcy mastitis

72%

Ruegg P. L.

Hoduj z Głową - Bydło

|

2014

|

nr 2

13

Immunoprofilaktyka zakażeń mykoplazmowych u bydła

72%

Nicholas R. A. J., Ayling R. D.

Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii

|

2009

|

tom 04

|

nr 2 Monografia

14

"Koci katar". Diagnostyka patogenów metodą PCR

58%

Heubner J.

Weterynaria w Praktyce

|

2009

|

tom 06

|

nr 04

15

Badania noworodkow lisow hodowlanych w kierunku zakazen wirusem CHV, Mycoplasma sp. oraz zarazenia Toxocara canis

58%

Smielewska-Los E., Klimentowski S., Kaszubkiewicz C., Pacon J.

Medycyna Weterynaryjna

|

1998

|

tom 54

|

nr 04

253-257

The purpose of the study was to establish the influence of canine herpesvirus (CHV), Mycoplasma sp. and Toxocara canis on new-born fox mortality. 25 new-born foxes died at the age of up to 5 days. They were derived from 5 blue and silver fox farms. Virologic investigations were done by means of direct IF method and virus isolation attemps. Hepatic, splenic and renal tissue suspensions were inoculated into monolayers of cell lines: A-72, MDCK, Vero, RK-13. PPLO (Difco) medium was used to isolate mycoplasmas. Parasitological examinations were performed by means of the larvoscopy technique. At autopsy lung congestion was observed in the all the examined pups. Renal haemorrhages in subcabsular region of the kidney were found in some pups. No microorganisms were isolated from basic bacteriological examinations of the pups. Females were free from brucellosis, leptospirosis and listeriosis. Low levels af antibodies against CHV were determined in some vixens. Histopathologically interstitial pneumonia was found in the all examined foxes. Canine herpesvirus was not isolated from any of the cases, as well as no Toxocara canis larvae were observed in lungs and livers. Mycoplasmas were isolated from the lungs of 5 pups derived from 2 farms. This suggests that mycoplasmas may cause interstitial pneumonia in new-born foxes.

16

Zaraza płucna bydła

58%

Glinski Z., Kostro K.

Hodowca Bydła

|

2007

|

nr 09

17

Mykoplazmy. Czynnik etiologiczny chorob ukladu oddechowego i krwi kotow

58%

Rzezutka A., Kozyra I., Mizak B.

Weterynaria w Praktyce

|

2007

|

tom 04

|

nr 4

42

18

Niewirusowe choroby ukladu oddechowego drobiu

58%

Emele J., Lohr J.

Magazyn Weterynaryjny

|

2002

|

tom 11

|

nr 06

60-63

19

Status immunologiczny kur reprodukcyjnych w fermie drobiu

58%

Trawinska B., Tymczyna L., Saba L.

Medycyna Weterynaryjna

|

2003

|

tom 59

|

nr 03

243-246

20

Mykoplazmy jako czynnik wywołujący zapalenie wymienia u krów mlecznych

58%

Bednarko-Mlynarczyk E., Szteyn J., Wiszniewska-Laszczych A., Liedtke K.

Medycyna Weterynaryjna

|

2013

|

tom 69

|

nr 07

Mycoplasma mastitis is one of the most pressing problems in herds of cows, especially in regions of increased milk production. Clinical signs in infected animals are not specific and the diagnosis of its causes should be based on laboratory testing. The classical scheme of investigation used in laboratories does not allow for their detection and should be extended to one of their methods of detection. Increased opportunities to detect mycoplasma are attained by proper storage of the milk samples.

Ograniczanie wyników

Zastosowanie odczynu ELISA do diagnostyki zakazen mykoplazmami u drobiu

Antybiotykooporność i mechanizmy jej powstawania w przypadku zakażeń Mycoplasma bovis u bydła

Czy organizmy prokariotyczne maja cytoszkielet?

Występowanie zakażeń mikoplazmami u gęsiorów i kaczorów piżmowych używanych jako dawcy nasienia w inseminacji

Zakazenia kur Mycoplasma gallisepticum/synoviae w swietle badan serologicznych

Szczepionka przeciwko bronchopneumonii cielat wywolanej przez Mycoplasma bovis

Profilaktyka zakazen Mycoplasma gallisepticum

Zwalczanie zespolu oddechowego swin

Spostrzezenia nad mozliwosciami klinicznego zastosowania nowej kombinacji antybiotykow - kolistyny ze spiramycyna

Wlasciwosci biologiczne Mycoplasma hyopneumoniae, czynnika etiologicznego mykoplazmowego zapalenia pluc

Szczepionka przeciwko mykoplazmozie kur

Sprawcy mastitis

Immunoprofilaktyka zakażeń mykoplazmowych u bydła

"Koci katar". Diagnostyka patogenów metodą PCR

Badania noworodkow lisow hodowlanych w kierunku zakazen wirusem CHV, Mycoplasma sp. oraz zarazenia Toxocara canis

Zaraza płucna bydła

Mykoplazmy. Czynnik etiologiczny chorob ukladu oddechowego i krwi kotow

Niewirusowe choroby ukladu oddechowego drobiu

Status immunologiczny kur reprodukcyjnych w fermie drobiu

Mykoplazmy jako czynnik wywołujący zapalenie wymienia u krów mlecznych