Wyniki wyszukiwania

1

Inhibition of hypoxia-induced tolerance to subsequent hyperoxic injury in lung cells by phosphatidyl inositol 3-kinase inhibitors: LY294002 and wortmannin

100%

Ahmad S., Ahmad A., Gerasmivoskaya E., Stenmark K. R., White C. W.

Cellular and Molecular Biology Letters

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2003

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tom 08

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nr 2A

2

Protein C activation peptide inhibits the expression of ICAM-1, VCAM-1, and interleukin-8 induced by TNF-alpha in human dermal microvascular endothelial cells

84%

Del Socorro Pina-Canseco M., Paez-Arenas A., Masso F., Perez-Campos E., Martinez-Cruz R., Hernandez-Cruz P., Majluf-Cruz A., Martinez-Cruz M., Perez-Campos Mayoral L., Perez-Santiago A. D., Zenteno E.

Folia Histochemica et Cytobiologica

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2012

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tom 50

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nr 3

3

Reduced ghrelin in endothelial cells plays important mechanistic role in aging-related impairment of angiogenesis

84%

Ahluwalia A., li A., Cheng G., Deng X., Tarnawski A. S.

Journal of Physiology and Pharmacology

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2009

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tom 60

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nr 2

29-34

Ghrelin, a hormone produced mainly by gastric mucosal cells stimulates growth hormone (GH) release. Ghrelin is also expressed in the endothelial cells of blood vessels suggesting its physiological role and a function in these cells. We recently demonstrated that ghrelin induces angiogenesis - new capillary blood vessel formation- in neonatal human microvascular endothelial cells (HMVECs). Angiogenesis is impaired in aging individuals both in vitro and in vivo, but the precise mechanism(s) of this phenomenon is unknown. We examined whether HMVECs derived from aging individuals (66 years and 90 years old), 66-HMVECs and 90-HMVECs have reduced ghrelin levels vs. neonatal (Neo) HMVECs and whether treatment with exogenous ghrelin can restore impaired in vitro angiogenesis on matrigel in aged HMVECs. Ghrelin levels were reduced in the aged HMVECs by 3.2-fold (p<0.05) compared to Neo-HMVECs. Angiogenesis was significantly decreased in the aged 66- and 90-HMVECs by 39.7% (p = 0.003) and 62.4% (p = 0.003), respectively compared to Neo-HMVECs. Treatment with exogenous ghrelin significantly reversed impaired angiogenesis in aged HMVECs with the EC50 0.05 nM. Ghrelin induced angiogenesis in Neo-HMVECs mainly through ERK2 activation. This study is the first demonstration that reduced ghrelin is one of the factors responsible for aging-related impairment of angiogenesis.

4

Activation of the metabolic sensor - AMP activated protein kinase reverses impairment of angiogenesis in aging myocardial microvascular endothelial cells. Implications for the aging heart

67%

Ahluwalia A., Tarnawski A. S.

Journal of Physiology and Pharmacology

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2011

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tom 62

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nr 5

5

Double-edged sword behaviour of gallic acid and its interaction with peroxidases in human microvascular endothelial cell culture (HMEC-1). Antioxidant and pro-oxidant effects

67%

Serrano J., Cipak A., Boada J., Gonzalo H., Cacabelos D., Cassanye A., Pamplona R., Zarkovic N., Portero-Otin M.

Acta Biochimica Polonica

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2010

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tom 57

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nr 2

A previous report from our group had shown in vitro a direct interaction between peroxidases and dietary antioxidants at physiological concentrations, where in the absence of H2O2, the antioxidants could serve as oxidizing substrates for the peroxidases. However, the physiological relevance of those findings had not been evaluated. The main objective of this study was to determine whether the oxidizing products produced in the interaction between peroxidase and gallic acid at a physiological concentration of 1 μM may promote cell death or survival in a human microvascular endothelial cell line (HMEC-1). Our findings suggested that gallic acid may show a double-edged sword behaviour, since in the absence of H2O2 it may have a pro-oxidant effect which may promote cell injury (evidenced by LDH, Crystal Violet and calcein AM viability/citotoxicity assays), while in the presence of H2O2, gallic acid may act as an antioxidant inhibiting oxidative species produced in the peroxidase cycle of peroxidases. These observations were confirmed with several oxidative stress biomarkers and the evaluation of the activation of cell survival pathways like AKT and MAPK/ERK.

Ograniczanie wyników

Inhibition of hypoxia-induced tolerance to subsequent hyperoxic injury in lung cells by phosphatidyl inositol 3-kinase inhibitors: LY294002 and wortmannin

Protein C activation peptide inhibits the expression of ICAM-1, VCAM-1, and interleukin-8 induced by TNF-alpha in human dermal microvascular endothelial cells

Reduced ghrelin in endothelial cells plays important mechanistic role in aging-related impairment of angiogenesis

Activation of the metabolic sensor - AMP activated protein kinase reverses impairment of angiogenesis in aging myocardial microvascular endothelial cells. Implications for the aging heart

Double-edged sword behaviour of gallic acid and its interaction with peroxidases in human microvascular endothelial cell culture (HMEC-1). Antioxidant and pro-oxidant effects